Gene description for DCAF16
Gene name DDB1 and CUL4 associated factor 16
Gene symbol DCAF16
Other names/aliases C4orf30
Species Homo sapiens
 Database cross references - DCAF16
ExoCarta ExoCarta_54876
Vesiclepedia VP_54876
Entrez Gene 54876
HGNC 25987
UniProt Q9NXF7  
 DCAF16 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DCAF16
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IBA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
 Experiment description of studies that identified DCAF16 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DCAF16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C6orf222  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SFRP4 6424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CDC14B 8555
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 FGF11  
Affinity Capture-MS Homo sapiens
8 GPS1 2873
Affinity Capture-MS Homo sapiens
9 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RBPJ 3516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SMAD7  
Affinity Capture-MS Homo sapiens
12 PRPS1 5631
Affinity Capture-MS Homo sapiens
13 C5orf24 134553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GLMN 11146
Affinity Capture-MS Homo sapiens
15 GREM2 64388
Affinity Capture-MS Homo sapiens
16 ECT2 1894
Affinity Capture-MS Homo sapiens
17 PRMT1 3276
Two-hybrid Homo sapiens
18 CCDC140  
Affinity Capture-MS Homo sapiens
19 CSNK1G2 1455
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TRIM41 90933
Affinity Capture-MS Homo sapiens
21 PSD2  
Affinity Capture-MS Homo sapiens
22 EN1  
Affinity Capture-MS Homo sapiens
23 UBA52 7311
Affinity Capture-MS Homo sapiens
24 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
25 PRMT6  
Two-hybrid Homo sapiens
26 PLEKHJ1  
Affinity Capture-MS Homo sapiens
27 CUL4B 8450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
28 NARFL 64428
Affinity Capture-MS Homo sapiens
29 Ddb1 13194
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
30 ZNF414  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SPANXN4  
Affinity Capture-MS Homo sapiens
32 DDA1  
Affinity Capture-MS Homo sapiens
33 SP110  
Affinity Capture-MS Homo sapiens
34 BARD1 580
Affinity Capture-MS Homo sapiens
35 FGF12  
Affinity Capture-MS Homo sapiens
36 TXNIP 10628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 COPS6 10980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SPIN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 FBXL6  
Affinity Capture-MS Homo sapiens
40 LDLRAP1 26119
Affinity Capture-MS Homo sapiens
41 COPS5 10987
Affinity Capture-MS Homo sapiens
42 DDB1 1642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
43 ZNF784  
Affinity Capture-MS Homo sapiens
44 TGIF2LY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 DGKG  
Affinity Capture-MS Homo sapiens
46 SYT2  
Affinity Capture-MS Homo sapiens
47 BMP4 652
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SMOC1 64093
Affinity Capture-MS Homo sapiens
49 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CUL4A 8451
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 ARHGAP19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ZBTB44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DCAF16 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Neddylation TAS Reactome
Post-translational protein modification TAS Reactome





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