Gene description for PRPS1
Gene name phosphoribosyl pyrophosphate synthetase 1
Gene symbol PRPS1
Other names/aliases ARTS
CMTX5
DFN2
DFNX1
PPRibP
PRS-I
PRSI
Species Homo sapiens
 Database cross references - PRPS1
ExoCarta ExoCarta_5631
Entrez Gene 5631
HGNC 9462
MIM 311850
UniProt P60891  
 PRPS1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PRPS1
Molecular Function
    ATP binding GO:0005524 IDA
    GDP binding GO:0019003 IEA
    ribose phosphate diphosphokinase activity GO:0004749 IMP
    magnesium ion binding GO:0000287 IEA
    protein homodimerization activity GO:0042803 IPI
    AMP binding GO:0016208 IEA
    ADP binding GO:0043531 IEA
    kinase activity GO:0016301 IEA
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IEA
Biological Process
    organ regeneration GO:0031100 IEA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 TAS
    pyrimidine nucleotide biosynthetic process GO:0006221 NAS
    hypoxanthine biosynthetic process GO:0046101 IMP
    nervous system development GO:0007399 IMP
    carbohydrate metabolic process GO:0005975 TAS
    phosphorylation GO:0016310 IEA
    AMP biosynthetic process GO:0006167 IEA
    small molecule metabolic process GO:0044281 TAS
    purine nucleotide biosynthetic process GO:0006164 IMP
    purine nucleobase metabolic process GO:0006144 IMP
    urate biosynthetic process GO:0034418 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    ribose phosphate diphosphokinase complex GO:0002189 IEA
 Experiment description of studies that identified PRPS1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PRPS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRPS1 5631
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 SPG21 51324
Two-hybrid Homo sapiens
3 PRPSAP1 5635
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 GORASP2 26003
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PRPS1 is involved
PathwayEvidenceSource
5-Phosphoribose 1-diphosphate biosynthesis TAS Reactome





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