Gene description for PLS1
Gene name plastin 1
Gene symbol PLS1
Other names/aliases -
Species Homo sapiens
 Database cross references - PLS1
ExoCarta ExoCarta_5357
Vesiclepedia VP_5357
Entrez Gene 5357
HGNC 9090
MIM 602734
UniProt Q14651  
 PLS1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
Urine 19056867    
 Gene ontology annotations for PLS1
Molecular Function
    structural constituent of cytoskeleton GO:0005200 TAS
    calcium ion binding GO:0005509 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    intestinal D-glucose absorption GO:0001951 IEA
    regulation of microvillus length GO:0032532 IEA
    positive regulation of multicellular organism growth GO:0040018 IEA
    actin filament bundle assembly GO:0051017 IBA
    actin filament network formation GO:0051639 IBA
    auditory receptor cell stereocilium organization GO:0060088 ISS
    vestibular receptor cell stereocilium organization GO:0060121 ISS
    terminal web assembly GO:1902896 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    actin filament GO:0005884 IBA
    plasma membrane GO:0005886 IDA
    brush border GO:0005903 ISS
    stereocilium GO:0032420 ISS
    actin filament bundle GO:0032432 IBA
    extracellular exosome GO:0070062 HDA
    terminal web GO:1990357 IEA
 Experiment description of studies that identified PLS1 in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 285
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PLS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
3 LMOD3  
Affinity Capture-MS Homo sapiens
4 MARCKS 4082
Proximity Label-MS Homo sapiens
5 NKD2 85409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ACTC1 70
Proximity Label-MS Homo sapiens
7 CRHBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MTMR14 64419
Affinity Capture-MS Homo sapiens
9 ANKRD39  
Affinity Capture-MS Homo sapiens
10 SOX2  
Affinity Capture-MS Homo sapiens
11 CLIC5 53405
Affinity Capture-MS Homo sapiens
12 DNAJB6 10049
Affinity Capture-MS Homo sapiens
13 JAZF1  
Affinity Capture-MS Homo sapiens
14 APEX1 328
Affinity Capture-MS Homo sapiens
15 RDX 5962
Co-fractionation Homo sapiens
16 RWDD2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GP1BB 2812
Affinity Capture-MS Homo sapiens
18 PRDX3 10935
Affinity Capture-MS Homo sapiens
19 ZNF321P  
Affinity Capture-MS Homo sapiens
20 ISYNA1 51477
Co-fractionation Homo sapiens
21 PRPS1 5631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 KRT31 3881
Affinity Capture-MS Homo sapiens
23 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PDIA6 10130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 LIPG 9388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 C9orf139  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SOCS3 9021
Affinity Capture-MS Homo sapiens
28 TIMM50 92609
Affinity Capture-MS Homo sapiens
29 FKBP14  
Co-fractionation Homo sapiens
30 PLS3 5358
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
31 FKBPL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 DVL2 1856
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 CHMP4C 92421
Affinity Capture-MS Homo sapiens
35 SPG21 51324
Affinity Capture-MS Homo sapiens
36 LCP1 3936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MT2A 4502
Affinity Capture-MS Homo sapiens
38 CAPZA1 829
Affinity Capture-MS Homo sapiens
39 GUCY2F  
Affinity Capture-MS Homo sapiens
40 OR10H3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SLAIN1 122060
Affinity Capture-MS Homo sapiens
42 MYH9 4627
Affinity Capture-MS Homo sapiens
43 HMGN1  
Co-fractionation Homo sapiens
44 FAM24B  
Affinity Capture-MS Homo sapiens
45 SLC25A32 81034
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ACTB 60
Proximity Label-MS Homo sapiens
47 AKAP7 9465
Affinity Capture-MS Homo sapiens
48 SNRNP27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 LYST 1130
Cross-Linking-MS (XL-MS) Homo sapiens
50 RCAN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 GSX1  
Affinity Capture-MS Homo sapiens
52 ANLN 54443
Affinity Capture-MS Homo sapiens
53 Myh9 17886
Affinity Capture-MS Mus musculus
54 TPK1  
Affinity Capture-MS Homo sapiens
55 ECT2 1894
Affinity Capture-MS Homo sapiens
56 NFKBIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CARTPT  
Affinity Capture-MS Homo sapiens
58 ACAD10  
Co-fractionation Homo sapiens
59 GALK2 2585
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SERPINB5 5268
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 KRT81  
Affinity Capture-MS Homo sapiens
62 ZMYM6  
Affinity Capture-MS Homo sapiens
63 USP47 55031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RPL12 6136
Cross-Linking-MS (XL-MS) Homo sapiens
65 NDC80 10403
Co-fractionation Homo sapiens
66 DDRGK1 65992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 QTRT1 81890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 FYTTD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 TBCA 6902
Affinity Capture-MS Homo sapiens
70 DHRS3 9249
Affinity Capture-MS Homo sapiens
71 MSN 4478
Co-fractionation Homo sapiens
72 CDK10  
Affinity Capture-MS Homo sapiens
73 C10orf32  
Affinity Capture-MS Homo sapiens
74 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
75 SPTAN1 6709
Co-fractionation Homo sapiens
76 DTD1 92675
Affinity Capture-MS Homo sapiens
77 PARP1 142
Co-fractionation Homo sapiens
78 SPRY2 10253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 MTPN 136319
Affinity Capture-MS Homo sapiens
80 EZR 7430
Co-fractionation Homo sapiens
81 CXCL14  
Affinity Capture-MS Homo sapiens
82 FSD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 FKBP4 2288
Co-fractionation Homo sapiens
84 GSKIP  
Affinity Capture-MS Homo sapiens
85 FCGR2A 2212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 PDCD2 5134
Affinity Capture-MS Homo sapiens
87 METTL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 INO80C  
Affinity Capture-MS Homo sapiens
89 GSTM3 2947
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 CDK13 8621
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 MRPL46  
Affinity Capture-MS Homo sapiens
92 CIT 11113
Affinity Capture-MS Homo sapiens
93 OTUD3 23252
Affinity Capture-MS Homo sapiens
94 SERBP1 26135
Affinity Capture-MS Homo sapiens
95 STC2 8614
Affinity Capture-MS Homo sapiens
96 UBE2Q2 92912
Affinity Capture-MS Homo sapiens
97 LMO1  
Affinity Capture-MS Homo sapiens
98 CRACR2A  
Affinity Capture-MS Homo sapiens
99 CCR1  
Affinity Capture-MS Homo sapiens
100 CUL4A 8451
Affinity Capture-MS Homo sapiens
101 CNPPD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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