Gene description for TIMM50
Gene name translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
Gene symbol TIMM50
Other names/aliases TIM50
TIM50L
Species Homo sapiens
 Database cross references - TIMM50
ExoCarta ExoCarta_92609
Entrez Gene 92609
HGNC 23656
MIM 607381
UniProt Q3ZCQ8  
 TIMM50 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TIMM50
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    interleukin-2 receptor binding GO:0005134 IDA
    phosphoprotein phosphatase activity GO:0004721 IDA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    ribonucleoprotein complex binding GO:0043021 IDA
    protein tyrosine phosphatase activity GO:0004725 IDA
Biological Process
    protein targeting to mitochondrion GO:0006626 TAS
    release of cytochrome c from mitochondria GO:0001836 IDA
    cellular protein metabolic process GO:0044267 TAS
    peptidyl-tyrosine dephosphorylation GO:0035335 IDA
    protein dephosphorylation GO:0006470 IDA
    mitochondrial membrane organization GO:0007006 IMP
Subcellular Localization
    nuclear speck GO:0016607 IDA
    mitochondrial inner membrane presequence translocase complex GO:0005744 IPI
    mitochondrion GO:0005739 IDA
    nucleoplasm GO:0005654 IDA
    mitochondrial inner membrane GO:0005743 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified TIMM50 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TIMM50
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PAFAH1B2 5049
Two-hybrid Homo sapiens
2 MAGED1  
Affinity Capture-MS Homo sapiens
3 TIMM23  
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
4 TNFRSF10B 8795
Two-hybrid Homo sapiens
5 PHB2 11331
Affinity Capture-MS Homo sapiens
6 PELO 53918
Affinity Capture-MS Homo sapiens
7 FTS  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TIMM50 is involved
PathwayEvidenceSource
Mitochondrial protein import TAS Reactome





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