Gene description for WASF3
Gene name WAS protein family, member 3
Gene symbol WASF3
Other names/aliases Brush-1
SCAR3
WAVE3
Species Homo sapiens
 Database cross references - WASF3
ExoCarta ExoCarta_10810
Vesiclepedia VP_10810
Entrez Gene 10810
HGNC 12734
MIM 605068
UniProt Q9UPY6  
 WASF3 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for WASF3
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    protein kinase A regulatory subunit binding GO:0034237 IBA
    Arp2/3 complex binding GO:0071933 IBA
Biological Process
    cytoskeleton organization GO:0007010 IMP
    regulation of cell shape GO:0008360 IMP
    oligodendrocyte development GO:0014003 IEA
    lamellipodium assembly GO:0030032 IEA
    actin cytoskeleton organization GO:0030036 IBA
    actin filament polymerization GO:0030041 TAS
    positive regulation of myelination GO:0031643 IEA
    protein-containing complex assembly GO:0065003 TAS
    modification of postsynaptic actin cytoskeleton GO:0098885 IEA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IBA
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    lamellipodium GO:0030027 IBA
    SCAR complex GO:0031209 IBA
    extracellular exosome GO:0070062 HDA
    glial cell projection GO:0097386 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified WASF3 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WASF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
2 CD47 961
Affinity Capture-MS Homo sapiens
3 TIMM50 92609
Affinity Capture-MS Homo sapiens
4 BRK1 55845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 KANK2 25959
Two-hybrid Homo sapiens
6 YWHAZ 7534
Affinity Capture-MS Homo sapiens
7 CPNE7 27132
Affinity Capture-MS Homo sapiens
8 YWHAB 7529
Affinity Capture-MS Homo sapiens
9 WASF2 10163
Affinity Capture-MS Homo sapiens
10 GTF2IRD1  
Affinity Capture-MS Homo sapiens
11 NCKAP1 10787
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Affinity Capture-MS Homo sapiens
13 KIAA1217 56243
Affinity Capture-MS Homo sapiens
14 ARHGAP12  
Affinity Capture-MS Homo sapiens
15 WDR33 55339
Affinity Capture-MS Homo sapiens
16 RYK 6259
Affinity Capture-MS Homo sapiens
17 BYSL 705
Two-hybrid Homo sapiens
18 FRA10AC1 118924
Affinity Capture-MS Homo sapiens
19 WASF1 8936
Affinity Capture-MS Homo sapiens
20 HOXB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CYFIP2 26999
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CAPZB 832
Affinity Capture-MS Homo sapiens
24 ABI2 10152
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 OGFR 11054
Affinity Capture-MS Homo sapiens
26 KIFC3 3801
Two-hybrid Homo sapiens
27 AMZ1  
Affinity Capture-MS Homo sapiens
28 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
29 ACTR3 10096
Reconstituted Complex Homo sapiens
30 RASAL3 64926
Two-hybrid Homo sapiens
31 CMTR1 23070
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CENPF 1063
Affinity Capture-MS Homo sapiens
33 SUPV3L1 6832
Affinity Capture-MS Homo sapiens
34 CAMK2D 817
Two-hybrid Homo sapiens
35 CYFIP1 23191
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CCDC53 51019
Affinity Capture-MS Homo sapiens
37 ABI1 10006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CKS1B  
Two-hybrid Homo sapiens
39 LPP 4026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ACTG1 71
Affinity Capture-MS Homo sapiens
41 EPS8L2 64787
Affinity Capture-MS Homo sapiens
42 KIAA1522 57648
Affinity Capture-MS Homo sapiens
43 WASL 8976
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which WASF3 is involved
PathwayEvidenceSource
Disease TAS Reactome
Disease IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
RAC1 GTPase cycle TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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