Gene description for CENPF
Gene name centromere protein F, 350/400kDa
Gene symbol CENPF
Other names/aliases CENF
PRO1779
hcp-1
Species Homo sapiens
 Database cross references - CENPF
ExoCarta ExoCarta_1063
Vesiclepedia VP_1063
Entrez Gene 1063
HGNC 1857
MIM 600236
UniProt P49454  
 CENPF identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CENPF
Molecular Function
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 NAS
    transcription factor binding GO:0008134 IPI
    protein homodimerization activity GO:0042803 IPI
    dynein binding GO:0045502 IDA
    protein C-terminus binding GO:0008022 IPI
Biological Process
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IMP
    cell proliferation GO:0008283 NAS
    small GTPase mediated signal transduction GO:0007264 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    response to drug GO:0042493 NAS
    regulation of cell cycle GO:0051726 TAS
    metaphase plate congression GO:0051310 IDA
    mitotic spindle assembly checkpoint GO:0007094 NAS
    regulation of striated muscle tissue development GO:0016202 ISS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    cell division GO:0051301 IEA
    chromosome segregation GO:0007059 NAS
    muscle organ development GO:0007517 IEA
    cell differentiation GO:0030154 IEA
    kinetochore assembly GO:0051382 NAS
    mitotic nuclear division GO:0007067 IEA
    DNA biosynthetic process GO:0071897 IEA
    mitotic cell cycle GO:0000278 TAS
    protein transport GO:0015031 IMP
Subcellular Localization
    midbody GO:0030496 IDA
    pronucleus GO:0045120 IEA
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    nuclear matrix GO:0016363 IDA
    kinetochore GO:0000776 IDA
    condensed chromosome outer kinetochore GO:0000940 TAS
    spindle pole GO:0000922 IDA
    spindle GO:0005819 IDA
    chromatin GO:0000785 NAS
    chromosome, centromeric region GO:0000775 IDA
    nuclear envelope GO:0005635 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CENPF in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD9|FLOT1
EV Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CENPF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TOP3B 8940
Two-hybrid Homo sapiens
2 BRAP  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
3 CENPF 1063
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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