Gene description for CENPF
Gene name centromere protein F, 350/400kDa
Gene symbol CENPF
Other names/aliases CENF
PRO1779
hcp-1
Species Homo sapiens
 Database cross references - CENPF
ExoCarta ExoCarta_1063
Vesiclepedia VP_1063
Entrez Gene 1063
HGNC 1857
MIM 600236
UniProt P49454  
 CENPF identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CENPF
Molecular Function
    chromatin binding GO:0003682 NAS
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IEA
    protein homodimerization activity GO:0042803 IPI
    dynein complex binding GO:0070840 IBA
    dynein complex binding GO:0070840 IDA
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    mitotic cell cycle GO:0000278 IBA
    mitotic cell cycle GO:0000278 IMP
    kidney development GO:0001822 IMP
    chromosome segregation GO:0007059 IMP
    chromosome segregation GO:0007059 NAS
    mitotic spindle assembly checkpoint signaling GO:0007094 NAS
    muscle organ development GO:0007517 IEA
    response to xenobiotic stimulus GO:0009410 NAS
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IBA
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IMP
    protein transport GO:0015031 IDA
    protein transport GO:0015031 IMP
    regulation of striated muscle tissue development GO:0016202 ISS
    ventricular system development GO:0021591 IMP
    cell differentiation GO:0030154 IEA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    cell division GO:0051301 IEA
    metaphase chromosome alignment GO:0051310 IBA
    metaphase chromosome alignment GO:0051310 IDA
    kinetochore assembly GO:0051382 NAS
    DNA biosynthetic process GO:0071897 IEA
Subcellular Localization
    chromosome, centromeric region GO:0000775 IBA
    chromosome, centromeric region GO:0000775 IDA
    kinetochore GO:0000776 IDA
    chromatin GO:0000785 NAS
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 IDA
    outer kinetochore GO:0000940 IDA
    outer kinetochore GO:0000940 TAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nuclear envelope GO:0005635 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IEA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    axoneme GO:0005930 IEA
    nuclear matrix GO:0016363 IDA
    midbody GO:0030496 IDA
    nuclear membrane GO:0031965 IDA
    ciliary basal body GO:0036064 IEA
    pronucleus GO:0045120 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    ciliary transition fiber GO:0097539 IMP
 Experiment description of studies that identified CENPF in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CENPF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 Kif19a  
Affinity Capture-MS Mus musculus
5 Sgol2  
Affinity Capture-MS Mus musculus
6 MAGEA3  
Affinity Capture-MS Homo sapiens
7 FAM167A  
Affinity Capture-MS Homo sapiens
8 SYCE1  
Affinity Capture-MS Homo sapiens
9 ACTC1 70
Proximity Label-MS Homo sapiens
10 BUB1B  
Co-fractionation Homo sapiens
11 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
12 MTMR9 66036
Affinity Capture-MS Homo sapiens
13 APEX1 328
Proximity Label-MS Homo sapiens
14 TMA16  
Affinity Capture-MS Homo sapiens
15 POLH  
Affinity Capture-MS Homo sapiens
16 CENPU  
Affinity Capture-MS Homo sapiens
17 MRFAP1L1  
Affinity Capture-MS Homo sapiens
18 RDX 5962
Proximity Label-MS Homo sapiens
19 DYRK1A 1859
Affinity Capture-MS Homo sapiens
20 COPS6 10980
Affinity Capture-MS Homo sapiens
21 Trp53bp1  
Affinity Capture-MS Mus musculus
22 PRC1 9055
Affinity Capture-MS Homo sapiens
23 NPM1 4869
Affinity Capture-MS Homo sapiens
24 ACER3  
Affinity Capture-MS Homo sapiens
25 OBSL1 23363
Affinity Capture-MS Homo sapiens
26 Pdlim5 56376
Affinity Capture-MS Mus musculus
27 Spast  
Affinity Capture-MS Mus musculus
28 GOPC 57120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ASF1A 25842
Proximity Label-MS Homo sapiens
30 WASF3 10810
Affinity Capture-MS Homo sapiens
31 KIF14 9928
Affinity Capture-MS Homo sapiens
32 MZT1  
Affinity Capture-MS Homo sapiens
33 FBXL5  
Affinity Capture-MS Homo sapiens
34 CCT2 10576
Cross-Linking-MS (XL-MS) Homo sapiens
35 ALK 238
Affinity Capture-MS Homo sapiens
36 MTCH2 23788
Proximity Label-MS Homo sapiens
37 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
39 TOP3B 8940
Two-hybrid Homo sapiens
40 CCDC33 80125
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 Pafah1b1 18472
Affinity Capture-MS Mus musculus
42 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
44 HECTD1 25831
Affinity Capture-MS Homo sapiens
45 RHOT2 89941
Proximity Label-MS Homo sapiens
46 RMDN3 55177
Proximity Label-MS Homo sapiens
47 ANLN 54443
Affinity Capture-MS Homo sapiens
48 PHLPP1  
Proximity Label-MS Homo sapiens
49 EGLN3  
Affinity Capture-MS Homo sapiens
50 SIRT7  
Affinity Capture-MS Homo sapiens
51 ESAM 90952
Affinity Capture-MS Homo sapiens
52 NFKBIA  
Affinity Capture-MS Homo sapiens
53 MEX3A  
Affinity Capture-RNA Homo sapiens
54 CUL3 8452
Affinity Capture-MS Homo sapiens
55 H1F0 3005
Affinity Capture-MS Homo sapiens
56 FNTA 2339
Affinity Capture-MS Homo sapiens
57 Cep152  
Affinity Capture-MS Mus musculus
58 Paxip1  
Affinity Capture-MS Mus musculus
59 MAPRE3  
Proximity Label-MS Homo sapiens
60 COG6 57511
Affinity Capture-MS Homo sapiens
61 MYC  
Affinity Capture-MS Homo sapiens
62 HECW2  
Affinity Capture-MS Homo sapiens
63 TERF2  
Affinity Capture-MS Homo sapiens
64 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
65 FNTB 2342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CTNNA1 1495
Proximity Label-MS Homo sapiens
67 NANOG  
Affinity Capture-MS Homo sapiens
68 MAP7D2 256714
Affinity Capture-MS Homo sapiens
69 TXLNA 200081
Affinity Capture-MS Homo sapiens
70 Anapc2  
Affinity Capture-MS Mus musculus
71 ARRB2 409
Affinity Capture-MS Homo sapiens
72 CCDC68  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 POFUT2 23275
Cross-Linking-MS (XL-MS) Homo sapiens
74 NUP133 55746
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
75 SVIL 6840
Two-hybrid Homo sapiens
76 IKBKG 8517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SEPT10 151011
Proximity Label-MS Homo sapiens
78 G3BP1 10146
Affinity Capture-MS Homo sapiens
79 CENPF 1063
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
80 NQO2 4835
Co-fractionation Homo sapiens
81 MKI67  
Affinity Capture-MS Homo sapiens
82 BRK1 55845
Affinity Capture-MS Homo sapiens
83 NUP107 57122
Affinity Capture-Western Homo sapiens
84 TERF1 7013
Affinity Capture-MS Homo sapiens
85 PPP1R13B  
Affinity Capture-MS Homo sapiens
86 RPL7A 6130
Cross-Linking-MS (XL-MS) Homo sapiens
87 KXD1 79036
Affinity Capture-MS Homo sapiens
88 NDEL1 81565
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
89 CUL7 9820
Affinity Capture-MS Homo sapiens
90 FBXO38 81545
Proximity Label-MS Homo sapiens
91 RPA3 6119
Proximity Label-MS Homo sapiens
92 CUL4A 8451
Affinity Capture-MS Homo sapiens
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