Gene description for RHOT2
Gene name ras homolog family member T2
Gene symbol RHOT2
Other names/aliases ARHT2
C16orf39
MIRO-2
MIRO2
RASL
Species Homo sapiens
 Database cross references - RHOT2
ExoCarta ExoCarta_89941
Entrez Gene 89941
HGNC 21169
MIM 613889
UniProt Q8IXI1  
 RHOT2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RHOT2
Molecular Function
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IEA
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IEA
Biological Process
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    cellular homeostasis GO:0019725 IMP
    metabolic process GO:0008152 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    mitochondrion transport along microtubule GO:0047497 IMP
    mitochondrial outer membrane permeabilization GO:0097345 IMP
Subcellular Localization
    integral component of mitochondrial outer membrane GO:0031307 IDA
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified RHOT2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RHOT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RHOT2 is involved
PathwayEvidenceSource
Rho GTPase cycle TAS Reactome





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