Gene description for EHBP1
Gene name EH domain binding protein 1
Gene symbol EHBP1
Other names/aliases HPC12
NACSIN
Species Homo sapiens
 Database cross references - EHBP1
ExoCarta ExoCarta_23301
Vesiclepedia VP_23301
Entrez Gene 23301
HGNC 29144
MIM 609922
UniProt Q8NDI1  
 EHBP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for EHBP1
Biological Process
    endocytosis GO:0006897 IEA
    protein transport GO:0015031 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    endosome GO:0005768 IEA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified EHBP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for EHBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CALML3 810
Affinity Capture-MS Homo sapiens
2 RNF43  
Proximity Label-MS Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 RAB35 11021
Proximity Label-MS Homo sapiens
5 RNASE7 84659
Affinity Capture-MS Homo sapiens
6 MTCH1 23787
Proximity Label-MS Homo sapiens
7 EBAG9 9166
Proximity Label-MS Homo sapiens
8 SERPINA12  
Affinity Capture-MS Homo sapiens
9 PACSIN1  
Two-hybrid Homo sapiens
10 METTL7A 25840
Proximity Label-MS Homo sapiens
11 NDUFS1 4719
Affinity Capture-MS Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 RAB9A 9367
Proximity Label-MS Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 GJA1 2697
Proximity Label-MS Homo sapiens
16 NUP155 9631
Proximity Label-MS Homo sapiens
17 LRRC59 55379
Proximity Label-MS Homo sapiens
18 POF1B 79983
Affinity Capture-MS Homo sapiens
19 S100A2 6273
Affinity Capture-MS Homo sapiens
20 MTCH2 23788
Proximity Label-MS Homo sapiens
21 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 RPAP2  
Affinity Capture-MS Homo sapiens
23 EHD2 30846
Reconstituted Complex Homo sapiens
24 EMD 2010
Proximity Label-MS Homo sapiens
25 DCTN2 10540
Proximity Label-MS Homo sapiens
26 FKBP8 23770
Proximity Label-MS Homo sapiens
27 RHOT2 89941
Proximity Label-MS Homo sapiens
28 CXADR 1525
Proximity Label-MS Homo sapiens
29 STX4 6810
Proximity Label-MS Homo sapiens
30 C11orf52 91894
Proximity Label-MS Homo sapiens
31 OCLN 100506658
Proximity Label-MS Homo sapiens
32 LYN 4067
Proximity Label-MS Homo sapiens
33 OCIAD1 54940
Proximity Label-MS Homo sapiens
34 MCAM 4162
Proximity Label-MS Homo sapiens
35 RAB4A 5867
Proximity Label-MS Homo sapiens
36 RAB3B 5865
Proximity Label-MS Homo sapiens
37 RAB5A 5868
Proximity Label-MS Homo sapiens
38 KHDRBS2  
Affinity Capture-MS Homo sapiens
39 LAMTOR1 55004
Proximity Label-MS Homo sapiens
40 RAB11A 8766
Proximity Label-MS Homo sapiens
41 SOAT1 6646
Affinity Capture-MS Homo sapiens
42 FIS1 51024
Proximity Label-MS Homo sapiens
43 CDH1 999
Proximity Label-MS Homo sapiens
44 MDM2  
Affinity Capture-MS Homo sapiens
45 ARID3A  
Affinity Capture-MS Homo sapiens
46 SEC62 7095
Proximity Label-MS Homo sapiens
47 B3GAT1  
Proximity Label-MS Homo sapiens
48 PTPN1 5770
Proximity Label-MS Homo sapiens
49 ACAP2 23527
Affinity Capture-MS Homo sapiens
50 COPS6 10980
Affinity Capture-MS Homo sapiens
51 METTL21B  
Affinity Capture-MS Homo sapiens
52 COPS5 10987
Affinity Capture-MS Homo sapiens
53 EPHA2 1969
Proximity Label-MS Homo sapiens
54 KRAS 3845
Proximity Label-MS Homo sapiens
55 RHOB 388
Proximity Label-MS Homo sapiens
56 KLK7 5650
Affinity Capture-MS Homo sapiens
57 DERL1 79139
Proximity Label-MS Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 CAV1 857
Proximity Label-MS Homo sapiens
60 CACYBP 27101
Cross-Linking-MS (XL-MS) Homo sapiens
61 SEC61B 10952
Proximity Label-MS Homo sapiens
62 FLOT1 10211
Proximity Label-MS Homo sapiens
63 STX6 10228
Proximity Label-MS Homo sapiens
64 KPRP 448834
Affinity Capture-MS Homo sapiens
65 RMDN3 55177
Proximity Label-MS Homo sapiens
66 SLC25A46 91137
Proximity Label-MS Homo sapiens
67 CELSR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EHBP1 is involved
No pathways found





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