Gene description for TTK
Gene name TTK protein kinase
Gene symbol TTK
Other names/aliases CT96
ESK
MPH1
MPS1
MPS1L1
PYT
Species Homo sapiens
 Database cross references - TTK
ExoCarta ExoCarta_7272
Vesiclepedia VP_7272
Entrez Gene 7272
HGNC 12401
MIM 604092
UniProt P33981  
 TTK identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for TTK
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine/tyrosine kinase activity GO:0004712 IBA
    protein tyrosine kinase activity GO:0004713 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3Y41 kinase activity GO:0035401 IEA
    identical protein binding GO:0042802 IEA
    kinetochore binding GO:0043515 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    spindle organization GO:0007051 TAS
    mitotic spindle organization GO:0007052 TAS
    chromosome segregation GO:0007059 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IDA
    positive regulation of cell population proliferation GO:0008284 TAS
    female meiosis chromosome segregation GO:0016321 IEA
    meiotic spindle assembly checkpoint signaling GO:0033316 IBA
    protein localization to kinetochore GO:0034501 IBA
    positive regulation of SMAD protein signal transduction GO:0060391 TAS
    repair of mitotic kinetochore microtubule attachment defect GO:0140273 IDA
    protein localization to meiotic spindle midzone GO:1903096 IEA
Subcellular Localization
    kinetochore GO:0000776 IBA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    spindle GO:0005819 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified TTK in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TTK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UTRN 7402
Affinity Capture-MS Homo sapiens
3 P2RY10  
Affinity Capture-MS Homo sapiens
4 CMTM8  
Affinity Capture-MS Homo sapiens
5 ACTC1 70
Proximity Label-MS Homo sapiens
6 FPR1  
Affinity Capture-MS Homo sapiens
7 LAMP2 3920
Affinity Capture-MS Homo sapiens
8 STX4 6810
Proximity Label-MS Homo sapiens
9 XIRP2  
Affinity Capture-MS Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 APEX1 328
Affinity Capture-RNA Homo sapiens
12 SLC18A2  
Affinity Capture-MS Homo sapiens
13 LAMTOR1 55004
Proximity Label-MS Homo sapiens
14 CDC16 8881
Affinity Capture-Western Homo sapiens
15 DYRK1A 1859
Affinity Capture-MS Homo sapiens
16 CHODL 140578
Affinity Capture-MS Homo sapiens
17 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CASC5  
Biochemical Activity Homo sapiens
19 FZR1  
Affinity Capture-Western Homo sapiens
20 IMPDH2 3615
Proximity Label-MS Homo sapiens
21 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 RCBTB2  
Affinity Capture-MS Homo sapiens
23 KRAS 3845
Proximity Label-MS Homo sapiens
24 CDC27 996
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 ESPL1  
Affinity Capture-MS Homo sapiens
26 GRPR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 WDR5 11091
Co-fractionation Homo sapiens
29 GPR17 2840
Affinity Capture-MS Homo sapiens
30 KIF14 9928
Affinity Capture-MS Homo sapiens
31 NINL  
Proximity Label-MS Homo sapiens
32 GEMIN5 25929
Affinity Capture-MS Homo sapiens
33 S100A2 6273
Affinity Capture-MS Homo sapiens
34 MIB1 57534
Proximity Label-MS Homo sapiens
35 UBE4B 10277
Affinity Capture-Western Homo sapiens
36 NAA50 80218
Affinity Capture-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 SLC39A9 55334
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DNAJC5 80331
Proximity Label-MS Homo sapiens
40 CDH1 999
Proximity Label-MS Homo sapiens
41 VCP 7415
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 RHOT2 89941
Proximity Label-MS Homo sapiens
45 ERI3 79033
Co-fractionation Homo sapiens
46 RMDN3 55177
Proximity Label-MS Homo sapiens
47 RPS6KA1 6195
Two-hybrid Homo sapiens
48 MTSS1L 92154
Affinity Capture-MS Homo sapiens
49 LPPR1  
Affinity Capture-MS Homo sapiens
50 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CXCR4 7852
Affinity Capture-MS Homo sapiens
52 GPR12  
Affinity Capture-MS Homo sapiens
53 CANX 821
Affinity Capture-MS Homo sapiens
54 RPL7A 6130
Affinity Capture-MS Homo sapiens
55 LRRC25  
Affinity Capture-MS Homo sapiens
56 NPTN 27020
Affinity Capture-MS Homo sapiens
57 PTH2R  
Affinity Capture-MS Homo sapiens
58 RAD52  
Biochemical Activity Homo sapiens
59 CUL3 8452
Affinity Capture-MS Homo sapiens
60 NPY2R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 EGFR 1956
Negative Genetic Homo sapiens
62 ANKRD36B  
Affinity Capture-MS Homo sapiens
63 RIPK4  
Affinity Capture-MS Homo sapiens
64 OCIAD1 54940
Proximity Label-MS Homo sapiens
65 NXF1 10482
Affinity Capture-RNA Homo sapiens
66 AURKB 9212
Affinity Capture-MS Homo sapiens
67 CDK2 1017
Biochemical Activity Homo sapiens
68 MAPK6  
Two-hybrid Homo sapiens
69 TREM1 54210
Affinity Capture-MS Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 NIN 51199
Proximity Label-MS Homo sapiens
72 NDC80 10403
Proximity Label-MS Homo sapiens
73 OPRL1 4987
Affinity Capture-MS Homo sapiens
74 HDLBP 3069
Co-fractionation Homo sapiens
75 RHOB 388
Proximity Label-MS Homo sapiens
76 PCM1 5108
Proximity Label-MS Homo sapiens
77 SLC22A4 6583
Affinity Capture-MS Homo sapiens
78 POLR2A 5430
Biochemical Activity Homo sapiens
79 AVPR2  
Affinity Capture-MS Homo sapiens
80 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 MAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 RNF43  
Proximity Label-MS Homo sapiens
83 KIF2A 3796
Affinity Capture-MS Homo sapiens
84 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
85 FASN 2194
Negative Genetic Homo sapiens
86 CDC20 991
Affinity Capture-Western Homo sapiens
87 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
89 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 SQSTM1 8878
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
91 TTK 7272
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
92 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
93 SHMT2 6472
Affinity Capture-RNA Homo sapiens
94 ESR1  
Affinity Capture-MS Homo sapiens
95 ANAPC2 29882
Proximity Label-MS Homo sapiens
96 IBTK 25998
Affinity Capture-MS Homo sapiens
97 CEP135  
Proximity Label-MS Homo sapiens
98 CLTA 1211
Proximity Label-MS Homo sapiens
99 Ttk  
Affinity Capture-MS Mus musculus
100 SNW1 22938
Affinity Capture-MS Homo sapiens
101 LPAR4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 VPS8 23355
Affinity Capture-MS Homo sapiens
103 HERC5 51191
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TTK is involved
No pathways found





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