Gene description for NAA50
Gene name N(alpha)-acetyltransferase 50, NatE catalytic subunit
Gene symbol NAA50
Other names/aliases MAK3
NAT13
NAT13P
NAT5
NAT5P
SAN
Species Homo sapiens
 Database cross references - NAA50
ExoCarta ExoCarta_80218
Entrez Gene 80218
HGNC 29533
MIM 610834
UniProt Q9GZZ1  
 NAA50 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for NAA50
Molecular Function
    peptidyl-lysine N-acetyltransferase activity, acting on acetyl phosphate as donor GO:0052858 IDA
    protein binding GO:0005515 IPI
    H4 histone acetyltransferase activity GO:0010485 IDA
    peptide alpha-N-acetyltransferase activity GO:0004596 IDA
Biological Process
    N-terminal protein amino acid acetylation GO:0006474 TAS
    mitotic sister chromatid cohesion, centromeric GO:0071962 IDA
    histone H4 acetylation GO:0043967 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    NatA complex GO:0031415 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified NAA50 in exosomes
1
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NAA50
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which NAA50 is involved
No pathways found





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