Gene description for KIFAP3
Gene name kinesin-associated protein 3
Gene symbol KIFAP3
Other names/aliases FLA3
KAP-1
KAP-3
KAP3
SMAP
Smg-GDS
dJ190I16.1
Species Homo sapiens
 Database cross references - KIFAP3
ExoCarta ExoCarta_22920
Vesiclepedia VP_22920
Entrez Gene 22920
HGNC 17060
MIM 601836
UniProt Q92845  
 KIFAP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for KIFAP3
Molecular Function
    protein binding GO:0005515 IPI
    kinesin binding GO:0019894 IPI
    protein phosphatase binding GO:0019903 IPI
    intraciliary transport particle B binding GO:0120170 IEA
Biological Process
    microtubule-based process GO:0007017 ISS
    microtubule-based movement GO:0007018 IBA
    signal transduction GO:0007165 TAS
    neuroblast proliferation GO:0007405 IEA
    negative regulation of neuroblast proliferation GO:0007406 IEA
    protein localization GO:0008104 IEA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    cilium organization GO:0044782 IBA
    positive regulation of calcium-dependent cell-cell adhesion GO:0046587 IEA
    protein-containing complex assembly GO:0065003 TAS
    thymocyte apoptotic process GO:0070242 IEA
    negative regulation of thymocyte apoptotic process GO:0070244 IEA
    plus-end-directed vesicle transport along microtubule GO:0072383 TAS
    anterograde dendritic transport of neurotransmitter receptor complex GO:0098971 IEA
Subcellular Localization
    condensed nuclear chromosome GO:0000794 IDA
    photoreceptor outer segment GO:0001750 IEA
    photoreceptor inner segment GO:0001917 IEA
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IEA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    spindle microtubule GO:0005876 IDA
    cilium GO:0005929 TAS
    axoneme GO:0005930 IBA
    microtubule cytoskeleton GO:0015630 IDA
    kinesin II complex GO:0016939 IBA
    kinesin II complex GO:0016939 IDA
    kinesin II complex GO:0016939 ISS
    photoreceptor connecting cilium GO:0032391 IEA
    dendrite cytoplasm GO:0032839 IEA
    ciliary transition zone GO:0035869 IBA
    ciliary basal body GO:0036064 IEA
    ciliary tip GO:0097542 TAS
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
    periciliary membrane compartment GO:1990075 IEA
 Experiment description of studies that identified KIFAP3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KIFAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 MUC13 56667
Affinity Capture-MS Homo sapiens
3 SMC3 9126
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 HBB 3043
Affinity Capture-MS Homo sapiens
5 CCSER2 54462
Two-hybrid Homo sapiens
6 RBM22 55696
Affinity Capture-MS Homo sapiens
7 GTF2IRD1  
Affinity Capture-MS Homo sapiens
8 FAM192A  
Affinity Capture-MS Homo sapiens
9 EFR3A 23167
Affinity Capture-MS Homo sapiens
10 CEP170 9859
Affinity Capture-MS Homo sapiens
11 MID1IP1  
Affinity Capture-MS Homo sapiens
12 APC  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 PAK2 5062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GNAQ 2776
Affinity Capture-MS Homo sapiens
15 PRPF3  
Affinity Capture-MS Homo sapiens
16 VHL  
Affinity Capture-Western Homo sapiens
17 BUD13  
Affinity Capture-MS Homo sapiens
18 MED24 9862
Affinity Capture-MS Homo sapiens
19 TAF1  
Affinity Capture-MS Homo sapiens
20 S100A13 6284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TAF5  
Affinity Capture-MS Homo sapiens
22 DDX23 9416
Affinity Capture-MS Homo sapiens
23 MARK2 2011
Two-hybrid Homo sapiens
24 ERICH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 GNAO1 2775
Affinity Capture-MS Homo sapiens
26 NAV1 89796
Two-hybrid Homo sapiens
27 YES1 7525
Affinity Capture-MS Homo sapiens
28 SRC 6714
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
30 Kif3c  
Affinity Capture-MS Mus musculus
31 PSMD5 5711
Affinity Capture-MS Homo sapiens
32 KIF3A 11127
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 RBM14 10432
Two-hybrid Homo sapiens
34 MEST 4232
Two-hybrid Homo sapiens
35 MRPS25 64432
Affinity Capture-MS Homo sapiens
36 BCAR1 9564
Affinity Capture-MS Homo sapiens
37 RUSC2 9853
Affinity Capture-MS Homo sapiens
38 NAA50 80218
Affinity Capture-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 GCC2 9648
Affinity Capture-MS Homo sapiens
41 TAF4 6874
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 RAP1A 5906
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SON 6651
Affinity Capture-MS Homo sapiens
45 CCNY 219771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RAP2A 5911
Affinity Capture-MS Homo sapiens
47 KIF3B 9371
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 DISC1 27185
Two-hybrid Homo sapiens
49 TAF7  
Affinity Capture-MS Homo sapiens
50 RAP1GDS1 5910
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
51 HIST1H3A 8350
Affinity Capture-Western Homo sapiens
52 SPTBN2 6712
Affinity Capture-MS Homo sapiens
53 TP53BP1 7158
Co-localization Homo sapiens
54 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
55 GTSE1 51512
Two-hybrid Homo sapiens
56 IFI30  
Affinity Capture-MS Homo sapiens
57 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 XAB2 56949
Affinity Capture-MS Homo sapiens
59 RBFOX1 54715
Two-hybrid Homo sapiens
60 DHX37  
Two-hybrid Homo sapiens
61 KPNA2 3838
Affinity Capture-MS Homo sapiens
62 SART1 9092
Affinity Capture-MS Homo sapiens
63 RBM26 64062
Affinity Capture-MS Homo sapiens
64 CCNL2 81669
Affinity Capture-MS Homo sapiens
65 MRPS17 51373
Affinity Capture-MS Homo sapiens
66 KIF3C 3797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 CEP350 9857
Affinity Capture-MS Homo sapiens
68 MAP3K10  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
69 OSBPL1A 114876
Affinity Capture-MS Homo sapiens
70 NAA10 8260
Two-hybrid Homo sapiens
71 NCKAP5  
Affinity Capture-MS Homo sapiens
72 VPS25 84313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 H2AFX 3014
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
74 NIPSNAP3A  
Two-hybrid Homo sapiens
75 DHX15 1665
Affinity Capture-MS Homo sapiens
76 NUDT21 11051
Two-hybrid Homo sapiens
77 MFHAS1 9258
Affinity Capture-MS Homo sapiens
78 COL4A1 1282
Affinity Capture-MS Homo sapiens
79 PRNP 5621
Two-hybrid Homo sapiens
80 PEX19 5824
Two-hybrid Homo sapiens
81 IQGAP3 128239
Affinity Capture-MS Homo sapiens
82 ORC3  
Affinity Capture-MS Homo sapiens
83 PARD6G 84552
Two-hybrid Homo sapiens
84 GAP43  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 NCF2 4688
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
86 HMGXB4 10042
Affinity Capture-MS Homo sapiens
87 MAP3K11 4296
Two-hybrid Homo sapiens
88 GBE1 2632
Affinity Capture-MS Homo sapiens
89 PRPF4 9128
Affinity Capture-MS Homo sapiens
90 AR 367
Two-hybrid Homo sapiens
91 RANBP2 5903
Affinity Capture-Western Homo sapiens
92 AIM1 202
Affinity Capture-MS Homo sapiens
93 INTU  
Affinity Capture-MS Homo sapiens
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