Gene description for KIFAP3
Gene name kinesin-associated protein 3
Gene symbol KIFAP3
Other names/aliases FLA3
KAP-1
KAP-3
KAP3
SMAP
Smg-GDS
dJ190I16.1
Species Homo sapiens
 Database cross references - KIFAP3
ExoCarta ExoCarta_22920
Vesiclepedia VP_22920
Entrez Gene 22920
HGNC 17060
MIM 601836
UniProt Q92845  
 KIFAP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for KIFAP3
Molecular Function
    kinesin binding GO:0019894 IPI
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of cell proliferation GO:0008285 IEA
    protein localization GO:0008104 IEA
    blood coagulation GO:0007596 TAS
    microtubule-based process GO:0007017 ISS
    membrane organization GO:0061024 TAS
    signal transduction GO:0007165 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    organelle organization GO:0006996 TAS
    microtubule-based movement GO:0007018 TAS
    positive regulation of calcium-dependent cell-cell adhesion GO:0046587 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    plus-end-directed vesicle transport along microtubule GO:0072383 TAS
    protein complex assembly GO:0006461 TAS
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    endoplasmic reticulum GO:0005783 IEA
    extracellular exosome GO:0070062 IDA
    ciliary basal body GO:0036064 IEA
    condensed nuclear chromosome GO:0000794 IDA
    centrosome GO:0005813 IDA
    kinesin II complex GO:0016939 ISS
    microtubule cytoskeleton GO:0015630 IDA
    primary cilium GO:0072372 TAS
    axoneme GO:0005930 IEA
    spindle microtubule GO:0005876 IDA
    photoreceptor connecting cilium GO:0032391 IEA
    periciliary membrane compartment GO:1990075 IEA
    cytosol GO:0005829 TAS
    intraciliary transport particle GO:0030990 IEA
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified KIFAP3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KIFAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF3B 9371
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 SRC 6714
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
3 SMC3 9126
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
5 APC  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 MAP3K10  
Two-hybrid Homo sapiens
7 MAP3K11 4296
Two-hybrid Homo sapiens
View the network image/svg+xml



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