Gene description for HBB
Gene name hemoglobin, beta
Gene symbol HBB
Other names/aliases CD113t-C
beta-globin
Species Homo sapiens
 Database cross references - HBB
ExoCarta ExoCarta_3043
Vesiclepedia VP_3043
Entrez Gene 3043
HGNC 4827
MIM 141900
UniProt P68871  
 HBB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for HBB
Molecular Function
    peroxidase activity GO:0004601 IBA
    peroxidase activity GO:0004601 IDA
    oxygen carrier activity GO:0005344 IBA
    oxygen carrier activity GO:0005344 NAS
    protein binding GO:0005515 IPI
    oxygen binding GO:0019825 IBA
    oxygen binding GO:0019825 IDA
    heme binding GO:0020037 IBA
    hemoglobin binding GO:0030492 IDA
    haptoglobin binding GO:0031720 IBA
    haptoglobin binding GO:0031720 IDA
    hemoglobin alpha binding GO:0031721 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of blood pressure GO:0008217 IEA
    carbon dioxide transport GO:0015670 NAS
    oxygen transport GO:0015671 IDA
    oxygen transport GO:0015671 IMP
    oxygen transport GO:0015671 NAS
    oxygen transport GO:0015671 TAS
    nitric oxide transport GO:0030185 IDA
    nitric oxide transport GO:0030185 NAS
    response to hydrogen peroxide GO:0042542 IDA
    hydrogen peroxide catabolic process GO:0042744 IBA
    hydrogen peroxide catabolic process GO:0042744 IDA
    positive regulation of nitric oxide biosynthetic process GO:0045429 NAS
    renal absorption GO:0070293 IMP
    platelet aggregation GO:0070527 HMP
    blood vessel diameter maintenance GO:0097746 IEA
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 TAS
    hemoglobin complex GO:0005833 IBA
    hemoglobin complex GO:0005833 IDA
    hemoglobin complex GO:0005833 IPI
    hemoglobin complex GO:0005833 NAS
    hemoglobin complex GO:0005833 TAS
    haptoglobin-hemoglobin complex GO:0031838 IBA
    haptoglobin-hemoglobin complex GO:0031838 IDA
    extracellular exosome GO:0070062 HDA
    endocytic vesicle lumen GO:0071682 TAS
    blood microparticle GO:0072562 HDA
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified HBB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
10
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
11
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
12
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
13
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 267
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
17
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
19
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
23
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
24
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
27
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
28
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HBB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
3 TRAF3IP3  
Affinity Capture-MS Homo sapiens
4 ARHGEF25 115557
Affinity Capture-MS Homo sapiens
5 GK3P  
Affinity Capture-MS Homo sapiens
6 C2CD4A  
Affinity Capture-MS Homo sapiens
7 SPRTN  
Affinity Capture-MS Homo sapiens
8 STATH  
Affinity Capture-MS Homo sapiens
9 SEP15 9403
Affinity Capture-MS Homo sapiens
10 SMG6  
Affinity Capture-RNA Homo sapiens
11 HBE1 3046
Affinity Capture-MS Homo sapiens
12 C1orf228  
Affinity Capture-MS Homo sapiens
13 ACAA1 30
Affinity Capture-MS Homo sapiens
14 LPAR6  
Affinity Capture-MS Homo sapiens
15 GC 2638
Affinity Capture-MS Homo sapiens
16 HIST1H2BJ 8970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SMG7  
Affinity Capture-RNA Homo sapiens
18 AGA 175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 BRD2  
Affinity Capture-MS Homo sapiens
20 SEMG2 6407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RIC8A 60626
Affinity Capture-MS Homo sapiens
22 HPX 3263
Affinity Capture-MS Homo sapiens
23 HP 3240
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
24 B3GNT2 10678
Affinity Capture-MS Homo sapiens
25 C9orf85  
Affinity Capture-MS Homo sapiens
26 MCM2 4171
Affinity Capture-MS Homo sapiens
27 ORM1 5004
Affinity Capture-MS Homo sapiens
28 PNKP 11284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RER1 11079
Affinity Capture-MS Homo sapiens
30 DAO  
Affinity Capture-MS Homo sapiens
31 RHOA 387
Affinity Capture-MS Homo sapiens
32 MED17  
Affinity Capture-MS Homo sapiens
33 UCHL5 51377
Reconstituted Complex Homo sapiens
34 MSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FNDC1 84624
Affinity Capture-MS Homo sapiens
36 SERPINA1 5265
Affinity Capture-MS Homo sapiens
37 SDCBP 6386
Affinity Capture-MS Homo sapiens
38 NHLH2  
Affinity Capture-MS Homo sapiens
39 RBM8A 9939
Affinity Capture-RNA Homo sapiens
40 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 UBE2V1 7335
Affinity Capture-MS Homo sapiens
42 AURKA 6790
Affinity Capture-MS Homo sapiens
43 GNG8  
Affinity Capture-MS Homo sapiens
44 POLD1 5424
Affinity Capture-MS Homo sapiens
45 CDYL 9425
Affinity Capture-MS Homo sapiens
46 PLXNB1 5364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 WHSC1 7468
Affinity Capture-MS Homo sapiens
48 ZDHHC19  
Affinity Capture-MS Homo sapiens
49 ERBB3 2065
Affinity Capture-MS Homo sapiens
50 PXK 54899
Affinity Capture-MS Homo sapiens
51 SSX2  
Affinity Capture-MS Homo sapiens
52 DHRS2 10202
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NDUFAF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 GLIPR1  
Affinity Capture-MS Homo sapiens
55 PABPC1 26986
Affinity Capture-RNA Homo sapiens
56 MATN2 4147
Affinity Capture-MS Homo sapiens
57 TTC19  
Affinity Capture-MS Homo sapiens
58 TRIM21 6737
Affinity Capture-MS Homo sapiens
59 TAF12  
Affinity Capture-MS Homo sapiens
60 JOSD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 GK  
Affinity Capture-MS Homo sapiens
62 ANXA9  
Affinity Capture-MS Homo sapiens
63 YWHAQ 10971
Affinity Capture-MS Homo sapiens
64 LCN1 3933
Affinity Capture-MS Homo sapiens
65 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
66 COPS3 8533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PDE7B  
Affinity Capture-MS Homo sapiens
68 HCN1  
Affinity Capture-MS Homo sapiens
69 SERPINA3 12
Affinity Capture-MS Homo sapiens
70 CYLD  
Affinity Capture-MS Homo sapiens
71 DKK2  
Affinity Capture-MS Homo sapiens
72 SDHD  
Affinity Capture-MS Homo sapiens
73 FAM189B  
Affinity Capture-MS Homo sapiens
74 SLX4  
Affinity Capture-MS Homo sapiens
75 RPL32 6161
Affinity Capture-MS Homo sapiens
76 FANCD2  
Affinity Capture-MS Homo sapiens
77 HMGCLL1  
Affinity Capture-MS Homo sapiens
78 SELT 51714
Two-hybrid Homo sapiens
79 ITLN2  
Affinity Capture-MS Homo sapiens
80 FOXO1  
Affinity Capture-MS Homo sapiens
81 HDAC9  
Affinity Capture-MS Homo sapiens
82 NR3C1 2908
Affinity Capture-MS Homo sapiens
83 DACH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 CP 1356
Affinity Capture-MS Homo sapiens
85 HSD17B10 3028
Affinity Capture-MS Homo sapiens
86 UPF3A  
Protein-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 MDM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PLLP  
Affinity Capture-MS Homo sapiens
89 GDAP1 54332
Co-fractionation Homo sapiens
90 KIAA1467 57613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CTSG 1511
Affinity Capture-MS Homo sapiens
92 CDK2 1017
Affinity Capture-MS Homo sapiens
93 CEP250 11190
Affinity Capture-MS Homo sapiens
94 MAPK6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 FRMD1  
Affinity Capture-MS Homo sapiens
96 BMP5 653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 NEFM 4741
Affinity Capture-MS Homo sapiens
98 MSL1 339287
Affinity Capture-MS Homo sapiens
99 SLC2A14 144195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 HBM  
Two-hybrid Homo sapiens
101 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 UPF3B 65109
Protein-RNA Homo sapiens
103 PAX3  
Affinity Capture-MS Homo sapiens
104 AKT1 207
Affinity Capture-MS Homo sapiens
105 IGLC1 3537
Affinity Capture-MS Homo sapiens
106 UPF2 26019
Affinity Capture-RNA Homo sapiens
107 STX17 55014
Affinity Capture-MS Homo sapiens
108 TMEM92  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 UBE2U  
Affinity Capture-MS Homo sapiens
110 GSTT1 2952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 PDLIM7 9260
Affinity Capture-MS Homo sapiens
112 PRR4 11272
Affinity Capture-MS Homo sapiens
113 PDE4B  
Protein-RNA Homo sapiens
114 TP53 7157
Affinity Capture-MS Homo sapiens
115 SCGB1D1  
Affinity Capture-MS Homo sapiens
116 SLC9A5  
Affinity Capture-MS Homo sapiens
117 IGLL5 100423062
Affinity Capture-MS Homo sapiens
118 PGPEP1 54858
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 LGALS3 3958
Affinity Capture-MS Homo sapiens
120 PNPLA1 285848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 HBA2 3040
Two-hybrid Homo sapiens
122 GRID1 2894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 ZNRF1  
Affinity Capture-MS Homo sapiens
124 DMWD  
Affinity Capture-MS Homo sapiens
125 EIF4A3 9775
Affinity Capture-RNA Homo sapiens
126 KIFAP3 22920
Affinity Capture-MS Homo sapiens
127 DDX58 23586
Affinity Capture-RNA Homo sapiens
128 MKI67  
Affinity Capture-MS Homo sapiens
129 TF 7018
Affinity Capture-MS Homo sapiens
130 ESR1  
Affinity Capture-MS Homo sapiens
131 CACUL1  
Affinity Capture-MS Homo sapiens
132 MGAT4B 11282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 ATP6V0C 527
Affinity Capture-MS Homo sapiens
134 FBLN7  
Affinity Capture-MS Homo sapiens
135 KLK10 5655
Affinity Capture-MS Homo sapiens
136 HBA1 3039
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
137 UPF1 5976
Affinity Capture-RNA Homo sapiens
138 LPAR2 9170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 HSDL1 83693
Affinity Capture-MS Homo sapiens
140 ETFDH 2110
Affinity Capture-MS Homo sapiens
141 DTWD1  
Affinity Capture-MS Homo sapiens
142 NUP210P1  
Affinity Capture-MS Homo sapiens
143 BNC1  
Affinity Capture-MS Homo sapiens
144 APCDD1  
Affinity Capture-MS Homo sapiens
145 DNAJA1 3301
Affinity Capture-MS Homo sapiens
146 GDPD1 284161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 CYTIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 GUCY1A3  
Affinity Capture-MS Homo sapiens
149 P2RX6  
Affinity Capture-MS Homo sapiens
150 HBZ  
Two-hybrid Homo sapiens
151 APOA1 335
Affinity Capture-MS Homo sapiens
152 C1QL3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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