Experiment description of studies that identified IGLL5 in exosomes

1 Experiment ID 494 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Osteoarthritic cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 496 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name Healthy cartilage Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

6 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for IGLL5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 IGLC2 3538 Affinity Capture-MS Homo sapiens 3 CNTNAP3   Affinity Capture-MS Homo sapiens 4 TK1 7083 Affinity Capture-MS Homo sapiens 5 IDUA 3425 Affinity Capture-MS Homo sapiens 6 NRSN1   Affinity Capture-MS Homo sapiens 7 DHRS12   Affinity Capture-MS Homo sapiens 8 ZNHIT2 741 Affinity Capture-MS Homo sapiens 9 MGAT5B   Two-hybrid Homo sapiens 10 PISD   Affinity Capture-MS Homo sapiens 11 EBF2   Affinity Capture-MS Homo sapiens 12 HBB 3043 Affinity Capture-MS Homo sapiens 13 FNDC5   Affinity Capture-MS Homo sapiens 14 HSPA5 3309 Affinity Capture-MS Homo sapiens 15 POTEF 728378 Affinity Capture-MS Homo sapiens 16 USB1   Affinity Capture-MS Homo sapiens 17 ITGA7 3679 Affinity Capture-MS Homo sapiens 18 ADGRG1 9289 Affinity Capture-MS Homo sapiens 19 CA8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 EGFL8   Affinity Capture-MS Homo sapiens 21 FGFR4 2264 Affinity Capture-MS Homo sapiens 22 SFXN4   Affinity Capture-MS Homo sapiens 23 SUN2 25777 Affinity Capture-MS Homo sapiens 24 PHF11 51131 Affinity Capture-MS Homo sapiens 25 FGFR3 2261 Affinity Capture-MS Homo sapiens 26 FNDC1 84624 Affinity Capture-MS Homo sapiens 27 SNX27 81609 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 IGLC3 3539 Affinity Capture-MS Homo sapiens 29 MYCN   Affinity Capture-MS Homo sapiens 30 FKBP10 60681 Affinity Capture-MS Homo sapiens 31 C18orf21   Affinity Capture-MS Homo sapiens 32 IGLC7 28834 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 OTUD1 220213 Affinity Capture-MS Homo sapiens 34 DDX19B 11269 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 DDX31   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 GNG8   Affinity Capture-MS Homo sapiens 37 NOMO1 23420 Affinity Capture-MS Homo sapiens 38 ST6GALNAC6 30815 Affinity Capture-MS Homo sapiens 39 HLA-F 3134 Affinity Capture-MS Homo sapiens 40 CSPG4 1464 Affinity Capture-MS Homo sapiens 41 IGLL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 ITLN2   Affinity Capture-MS Homo sapiens 43 NTRK1 4914 Affinity Capture-MS Homo sapiens 44 SSUH2   Affinity Capture-MS Homo sapiens 45 ALDH3A1 218 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 PCSK9 255738 Affinity Capture-MS Homo sapiens 47 ART5   Affinity Capture-MS Homo sapiens 48 PLXNA3 55558 Affinity Capture-MS Homo sapiens 49 GJB5   Affinity Capture-MS Homo sapiens 50 GUSB 2990 Affinity Capture-MS Homo sapiens 51 RHBDD1 84236 Affinity Capture-MS Homo sapiens 52 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 53 FANCD2   Affinity Capture-MS Homo sapiens 54 NEO1 4756 Affinity Capture-MS Homo sapiens 55 CD151 977 Affinity Capture-MS Homo sapiens 56 AGPAT1 10554 Affinity Capture-MS Homo sapiens 57 TMEM39B   Affinity Capture-MS Homo sapiens 58 PINK1   Affinity Capture-MS Homo sapiens 59 ASCC1 51008 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 TMEM176B 28959 Affinity Capture-MS Homo sapiens 61 RLIM 51132 Affinity Capture-MS Homo sapiens 62 HDAC9   Affinity Capture-MS Homo sapiens 63 LOXL2 4017 Affinity Capture-MS Homo sapiens 64 EGFR 1956 Affinity Capture-MS Homo sapiens 65 TRIM68 55128 Affinity Capture-MS Homo sapiens 66 SUZ12   Affinity Capture-MS Homo sapiens 67 SUSD3 203328 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 FRMD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 69 CST4 1472 Affinity Capture-MS Homo sapiens 70 TADA2A   Affinity Capture-MS Homo sapiens 71 TMEM132A 54972 Affinity Capture-MS Homo sapiens 72 CNTNAP3B   Affinity Capture-MS Homo sapiens 73 CSNK2A2 1459 Affinity Capture-MS Homo sapiens 74 RIBC1   Affinity Capture-MS Homo sapiens 75 RUNX2   Affinity Capture-MS Homo sapiens 76 EEF1A2 1917 Affinity Capture-MS Homo sapiens 77 TSPAN11 441631 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 78 RNF2   Affinity Capture-MS Homo sapiens 79 SEMA4G   Affinity Capture-MS Homo sapiens 80 STX17 55014 Affinity Capture-MS Homo sapiens 81 RIPPLY3   Affinity Capture-MS Homo sapiens 82 UBE2U   Affinity Capture-MS Homo sapiens 83 TTC13   Affinity Capture-MS Homo sapiens 84 CIR1   Affinity Capture-MS Homo sapiens 85 PLXNB2 23654 Affinity Capture-MS Homo sapiens 86 TTC17 55761 Affinity Capture-MS Homo sapiens 87 IGLC6 3542 Affinity Capture-MS Homo sapiens 88 SCGB1D1   Affinity Capture-MS Homo sapiens 89 ULBP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 90 GABPA 2551 Affinity Capture-MS Homo sapiens 91 TIMM10   Affinity Capture-MS Homo sapiens 92 SSBP2   Affinity Capture-MS Homo sapiens 93 PTDSS1 9791 Affinity Capture-MS Homo sapiens 94 HBQ1   Affinity Capture-MS Homo sapiens 95 SEMA3B 7869 Affinity Capture-MS Homo sapiens 96 MBNL1 4154 Affinity Capture-MS Homo sapiens 97 EZH2   Affinity Capture-MS Homo sapiens 98 KLK10 5655 Affinity Capture-MS Homo sapiens 99 TRIM31   Affinity Capture-MS Homo sapiens 100 ZG16B 124220 Affinity Capture-MS Homo sapiens 101 GOT1 2805 Affinity Capture-MS Homo sapiens 102 OLFM2   Affinity Capture-MS Homo sapiens 103 FOXN3 1112 Affinity Capture-MS Homo sapiens 104 LAMA5 3911 Affinity Capture-MS Homo sapiens 105 ITGA9 3680 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 106 PRKX 5613 Affinity Capture-MS Homo sapiens 107 OR2A4 79541 Affinity Capture-MS Homo sapiens 108 MATN2 4147 Affinity Capture-MS Homo sapiens 109 CCR1   Affinity Capture-MS Homo sapiens 110 MAN2B2 23324 Affinity Capture-MS Homo sapiens 111 CRLF1 9244 Affinity Capture-MS Homo sapiens View the network

Pathways in which IGLL5 is involved

No pathways found