Experiment description of studies that identified ADGRG1 in sEVs

1 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

2 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for ADGRG1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 LY86   Affinity Capture-MS Homo sapiens 2 TMEM170A   Affinity Capture-MS Homo sapiens 3 FBXO2 26232 Affinity Capture-MS Homo sapiens 4 ARRB2 409 Affinity Capture-Western Homo sapiens 5 ELSPBP1   Affinity Capture-MS Homo sapiens 6 BTNL2   Affinity Capture-MS Homo sapiens 7 TMEM106A   Affinity Capture-MS Homo sapiens 8 KLK15   Affinity Capture-MS Homo sapiens 9 C1QTNF9B   Affinity Capture-MS Homo sapiens 10 PCDHB7   Affinity Capture-MS Homo sapiens 11 HLA-G 3135 Affinity Capture-MS Homo sapiens 12 GP9 2815 Affinity Capture-MS Homo sapiens 13 HLA-DRA 3122 Affinity Capture-MS Homo sapiens 14 IGLL5 100423062 Affinity Capture-MS Homo sapiens 15 TSHR 7253 Affinity Capture-MS Homo sapiens 16 EFNA2   Affinity Capture-MS Homo sapiens 17 GGH 8836 Affinity Capture-MS Homo sapiens 18 ADRB2   PCA Homo sapiens Affinity Capture-Luminescence Homo sapiens 19 PDGFRA 5156 Affinity Capture-MS Homo sapiens 20 PRG2   Affinity Capture-MS Homo sapiens 21 MFAP4 4239 Affinity Capture-MS Homo sapiens 22 CBLN4   Affinity Capture-MS Homo sapiens 23 DUOXA2   Affinity Capture-MS Homo sapiens 24 CCR9   Two-hybrid Homo sapiens 25 FBXO6 26270 Affinity Capture-MS Homo sapiens 26 SLURP1   Affinity Capture-MS Homo sapiens 27 IL5RA   Affinity Capture-MS Homo sapiens 28 SFTPC   Affinity Capture-MS Homo sapiens 29 EPHA7 2045 Affinity Capture-MS Homo sapiens 30 LYZL1   Affinity Capture-MS Homo sapiens 31 VIPR2   Affinity Capture-MS Homo sapiens 32 BRICD5   Affinity Capture-MS Homo sapiens 33 SDF2L1 23753 Affinity Capture-MS Homo sapiens 34 SPPL2B 56928 Affinity Capture-MS Homo sapiens 35 PSCA 8000 Affinity Capture-MS Homo sapiens 36 GPR182   Affinity Capture-MS Homo sapiens 37 ALPP 250 Affinity Capture-MS Homo sapiens 38 DNAJB9 4189 Affinity Capture-MS Homo sapiens 39 NAGA 4668 Affinity Capture-MS Homo sapiens 40 HM13 81502 Affinity Capture-MS Homo sapiens 41 C5AR1   Affinity Capture-MS Homo sapiens 42 LYPD1   Affinity Capture-MS Homo sapiens 43 NXPH3   Affinity Capture-MS Homo sapiens 44 DRD2   Two-hybrid Homo sapiens 45 ST8SIA5   Affinity Capture-MS Homo sapiens 46 ADAM18   Affinity Capture-MS Homo sapiens 47 PTCH1   Affinity Capture-MS Homo sapiens 48 OR1D4   Affinity Capture-MS Homo sapiens 49 CLEC4E   Affinity Capture-MS Homo sapiens 50 CST9L   Affinity Capture-MS Homo sapiens 51 ZACN   Affinity Capture-MS Homo sapiens 52 LIPG 9388 Affinity Capture-MS Homo sapiens View the network

Pathways in which ADGRG1 is involved

No pathways found