ExoCarta: Gene summary

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Gene description for DNAJB9
Gene name	DnaJ (Hsp40) homolog, subfamily B, member 9
Gene symbol	DNAJB9
Other names/aliases	ERdj4 MDG-1 MDG1 MST049 MSTP049
Species	Homo sapiens
Database cross references - DNAJB9
ExoCarta	ExoCarta_4189
Vesiclepedia	VP_4189
Entrez Gene	4189
HGNC	6968
MIM	602634
UniProt	Q9UBS3
DNAJB9 identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Saliva	19199708

Gene ontology annotations for DNAJB9

Molecular Function
	protein binding	GO:0005515	IPI
	Hsp70 protein binding	GO:0030544	IEA
	protein-folding chaperone binding	GO:0051087	IBA
	protein-folding chaperone binding	GO:0051087	ISS
	misfolded protein binding	GO:0051787	IBA
	misfolded protein binding	GO:0051787	IDA
Biological Process
	positive regulation of immunoglobulin production	GO:0002639	ISS
	response to unfolded protein	GO:0006986	IEA
	B cell differentiation	GO:0030183	ISS
	response to endoplasmic reticulum stress	GO:0034976	ISS
	ERAD pathway	GO:0036503	IBA
	ERAD pathway	GO:0036503	IMP
	negative regulation of IRE1-mediated unfolded protein response	GO:1903895	ISS
Subcellular Localization
	nucleolus	GO:0005730	ISS
	cytoplasm	GO:0005737	ISS
	endoplasmic reticulum	GO:0005783	IBA
	endoplasmic reticulum	GO:0005783	IDA
	endoplasmic reticulum lumen	GO:0005788	IEA
	endoplasmic reticulum membrane	GO:0005789	TAS
	extracellular exosome	GO:0070062	HDA

Experiment description of studies that identified DNAJB9 in exosomes

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

MISEV standards
✔ IEM	Biophysical techniques
✔ TSG101\|Alix\|CD63\|CD81	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

19199708

Organism

Homo sapiens

Experiment description

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

Authors

"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"

Journal name

JPR

Publication year

2009

Sample

Saliva

Sample name

Saliva

Isolation/purification methods

Differential centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy

Protein-protein interactions for DNAJB9

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

GLT6D1

Affinity Capture-MS

Homo sapiens

ST8SIA3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SGSH

6448

Affinity Capture-MS

Homo sapiens

CELSR1

9620

Affinity Capture-MS

Homo sapiens

CEACAM8

1088

Affinity Capture-MS

Homo sapiens

SEP15

9403

Proximity Label-MS

Homo sapiens

MAN2B1

4125

Affinity Capture-MS

Homo sapiens

RXFP1

Affinity Capture-MS

Homo sapiens

UGGT2

55757

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

IDUA

3425

Affinity Capture-MS

Homo sapiens

NUCB2

4925

Proximity Label-MS

Homo sapiens

TOR1B

27348

Affinity Capture-MS

Homo sapiens

RAMP1

Affinity Capture-MS

Homo sapiens

TLR7

Affinity Capture-MS

Homo sapiens

ECEL1

Affinity Capture-MS

Homo sapiens

JMJD8

Affinity Capture-MS

Homo sapiens

FGF3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LOXL4

84171

Affinity Capture-MS

Homo sapiens

THSD4

79875

Affinity Capture-MS

Homo sapiens

APP

351

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

POMGNT2

Affinity Capture-MS

Homo sapiens

FGFR3

2261

Affinity Capture-MS

Homo sapiens

PLOD3

8985

Proximity Label-MS

Homo sapiens

SCARA3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LRP5

4041

Affinity Capture-MS

Homo sapiens

CEMIP

57214

Affinity Capture-MS

Homo sapiens

ADGRG1

9289

Affinity Capture-MS

Homo sapiens

CLEC18A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RNASE4

6038

Affinity Capture-MS

Homo sapiens

RCN2

5955

Proximity Label-MS

Homo sapiens

EGFL8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

P3H3

10536

Affinity Capture-MS

Homo sapiens

NUP210

23225

Proximity Label-MS

Homo sapiens

FGFR4

2264

Affinity Capture-MS

Homo sapiens

ST14

6768

Affinity Capture-MS

Homo sapiens

MARCH5

Affinity Capture-MS

Homo sapiens

DDOST

1650

Proximity Label-MS

Homo sapiens

CNTNAP3

Affinity Capture-MS

Homo sapiens

HSP90B1

7184

Proximity Label-MS

Homo sapiens

FAM136A

84908

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SUN2

25777

Affinity Capture-MS

Homo sapiens

NLGN2

57555

Affinity Capture-MS

Homo sapiens

PCSK6

5046

Affinity Capture-MS

Homo sapiens

PDIA6

10130

Proximity Label-MS

Homo sapiens

UXS1

80146

Affinity Capture-MS

Homo sapiens

F10

2159

Affinity Capture-MS

Homo sapiens

PRDX4

10549

Proximity Label-MS

Homo sapiens

SCNN1A

Affinity Capture-MS

Homo sapiens

WFDC6

Affinity Capture-MS

Homo sapiens

CALR

811

Proximity Label-MS

Homo sapiens

MAN2B2

23324

Affinity Capture-MS

Homo sapiens

TLL2

Affinity Capture-MS

Homo sapiens

P4HTM

Affinity Capture-MS

Homo sapiens

CTAGE5

4253

Affinity Capture-MS

Homo sapiens

P3H4

10609

Affinity Capture-MS

Homo sapiens

MYL6

4637

Two-hybrid

Homo sapiens

EMC1

23065

Proximity Label-MS

Homo sapiens

CHST12

55501

Affinity Capture-MS

Homo sapiens

LMAN1

3998

Proximity Label-MS

Homo sapiens

PRF1

Affinity Capture-MS

Homo sapiens

TNFSF11

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PLAUR

5329

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MANSC1

54682

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PPIB

5479

Proximity Label-MS

Homo sapiens

NOMO1

23420

Affinity Capture-MS

Homo sapiens

COLEC10

10584

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TMED6

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

IKBIP

121457

Proximity Label-MS

Homo sapiens

FAM71C

Two-hybrid

Homo sapiens

LRIG2

Affinity Capture-MS

Homo sapiens

IGF1R

3480

Affinity Capture-MS

Homo sapiens

PCSK5

5125

Affinity Capture-MS

Homo sapiens

TMEM43

79188

Affinity Capture-MS

Homo sapiens

TCEA2

Two-hybrid

Homo sapiens

GALNT12

79695

Affinity Capture-MS

Homo sapiens

PROZ

8858

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HPN

Affinity Capture-MS

Homo sapiens

DYNLL1

8655

Two-hybrid

Homo sapiens

PBXIP1

57326

Affinity Capture-MS

Homo sapiens

MAN2A2

4122

Affinity Capture-MS

Homo sapiens

VCP

7415

Affinity Capture-Western

Homo sapiens

PLK1

5347

Two-hybrid

Homo sapiens

MIA3

375056

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PLXDC2

84898

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

P4HB

5034

Proximity Label-MS

Homo sapiens

NAGLU

4669

Affinity Capture-MS

Homo sapiens

GANAB

23193

Proximity Label-MS

Homo sapiens

ADAM19

8728

Affinity Capture-MS

Homo sapiens

B4GALT3

8703

Affinity Capture-MS

Homo sapiens

DGCR2

9993

Affinity Capture-MS

Homo sapiens

TMEM131

Affinity Capture-MS

Homo sapiens

METRNL

284207

Affinity Capture-MS

Homo sapiens

TMPRSS13

Affinity Capture-MS

Homo sapiens

CERCAM

51148

Affinity Capture-MS

Homo sapiens

ZACN

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RFK

55312

Two-hybrid

Homo sapiens

BRINP3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C7orf34

Affinity Capture-MS

Homo sapiens

MBTPS1

8720

Affinity Capture-MS

Homo sapiens

100

MCOLN2

Affinity Capture-MS

Homo sapiens

101

GRIA3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

102

HSPA5

3309

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

103

TRHDE

29953

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

104

NHLRC3

387921

Affinity Capture-MS

Homo sapiens

105

TMEM30A

55754

Affinity Capture-MS

Homo sapiens

106

CANX

821

Proximity Label-MS

Homo sapiens

107

MUS81

Synthetic Growth Defect

Homo sapiens

108

UGGT1

56886

Proximity Label-MS

Homo sapiens

109

CHST6

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

DUOXA2

Affinity Capture-MS

Homo sapiens

111

TOR3A

64222

Affinity Capture-MS

Homo sapiens

112

ATP5H

10476

Proximity Label-MS

Homo sapiens

113

PTH2R

Affinity Capture-MS

Homo sapiens

114

PDGFRA

5156

Affinity Capture-MS

Homo sapiens

115

LAMB1

3912

Affinity Capture-MS

Homo sapiens

116

BCAP31

10134

Proximity Label-MS

Homo sapiens

117

LOXL2

4017

Affinity Capture-MS

Homo sapiens

118

PKD2L2

27039

Affinity Capture-MS

Homo sapiens

119

PDIA3

2923

Proximity Label-MS

Homo sapiens

120

LRRC4C

Affinity Capture-MS

Homo sapiens

121

LYZL1

Affinity Capture-MS

Homo sapiens

122

H6PD

9563

Affinity Capture-MS

Homo sapiens

123

ERP29

10961

Proximity Label-MS

Homo sapiens

124

RCN1

5954

Proximity Label-MS

Homo sapiens

125

THBS1

7057

Affinity Capture-MS

Homo sapiens

126

PRKCSH

5589

Proximity Label-MS

Homo sapiens

127

LNPEP

4012

Affinity Capture-MS

Homo sapiens

128

GALNT18

Affinity Capture-MS

Homo sapiens

129

CHRNB4

Affinity Capture-MS

Homo sapiens

130

PTPRK

5796

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

131

TXNDC5

81567

Proximity Label-MS

Homo sapiens

132

P2RX5

5026

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

133

DNAJC3

5611

Affinity Capture-MS

Homo sapiens

134

HYOU1

10525

Proximity Label-MS

Homo sapiens

135

DPM2

8818

Affinity Capture-MS

Homo sapiens

136

ENTPD2

954

Affinity Capture-MS

Homo sapiens

137

HLA-DQB1

3119

Affinity Capture-MS

Homo sapiens

138

KIAA0368

23392

Two-hybrid

Homo sapiens

139

PTPRU

Affinity Capture-MS

Homo sapiens

140

PCYOX1

51449

Affinity Capture-MS

Homo sapiens

141

BMP1

649

Affinity Capture-MS

Homo sapiens

142

LDLR

3949

Negative Genetic

Homo sapiens

143

FSCN1

6624

Two-hybrid

Homo sapiens

144

GABRD

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

145

KCNK16

Affinity Capture-MS

Homo sapiens

146

SGCD

6444

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

147

GSR

2936

Proximity Label-MS

Homo sapiens

148

ADAM10

102

Affinity Capture-MS

Homo sapiens

149

LYZL2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

150

TTC13

Affinity Capture-MS

Homo sapiens

151

SEL1L3

Affinity Capture-MS

Homo sapiens

152

SLC16A8

Two-hybrid

Homo sapiens

153

MR1

Affinity Capture-MS

Homo sapiens

154

SUN1

23353

Affinity Capture-MS

Homo sapiens

155

FASN

2194

Negative Genetic

Homo sapiens

156

TTC17

55761

Affinity Capture-MS

Homo sapiens

157

LAMB3

3914

Affinity Capture-MS

Homo sapiens

158

FOXRED2

Affinity Capture-MS

Homo sapiens

159

POTEF

728378

Affinity Capture-MS

Homo sapiens

160

SEMA6B

10501

Affinity Capture-MS

Homo sapiens

161

TCERG1

10915

Affinity Capture-MS

Homo sapiens

162

KDELC1

79070

Affinity Capture-MS

Homo sapiens

163

CALU

813

Proximity Label-MS

Homo sapiens

164

SEMA3C

10512

Affinity Capture-MS

Homo sapiens

165

TNFSF14

Affinity Capture-MS

Homo sapiens

166

CLPTM1

1209

Affinity Capture-MS

Homo sapiens

167

EDEM1

Affinity Capture-MS

Homo sapiens

168

CHST5

Affinity Capture-MS

Homo sapiens

169

OXSR1

9943

Affinity Capture-MS

Homo sapiens

170

ELAVL1

1994

Affinity Capture-RNA

Homo sapiens

171

COL18A1

80781

Affinity Capture-MS

Homo sapiens

172

CHST14

113189

Affinity Capture-MS

Homo sapiens

173

PDIA4

9601

Proximity Label-MS

Homo sapiens

174

OLFM4

10562

Affinity Capture-MS

Homo sapiens

175

EDEM3

80267

Affinity Capture-MS

Homo sapiens

176

EDEM2

Affinity Capture-MS

Homo sapiens

177

AGRN

375790

Affinity Capture-MS

Homo sapiens

178

SFTPC

Affinity Capture-Western

Homo sapiens

179

SEMA3B

7869

Affinity Capture-MS

Homo sapiens

180

OGFOD3

79701

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

181

GNB1L

54584

Proximity Label-MS

Homo sapiens

182

LRPAP1

4043

Proximity Label-MS

Homo sapiens

183

EXT2

2132

Affinity Capture-MS

Homo sapiens

184

NAB2

4665

Affinity Capture-MS

Homo sapiens

185

POMK

Affinity Capture-MS

Homo sapiens

186

FAM172A

Affinity Capture-MS

Homo sapiens

187

OLFM2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

188

LAMA5

3911

Affinity Capture-MS

Homo sapiens

189

CST11

Affinity Capture-MS

Homo sapiens

190

STT3B

201595

Proximity Label-MS

Homo sapiens

191

COLGALT1

79709

Proximity Label-MS

Homo sapiens

192

SEMA4G

Affinity Capture-MS

Homo sapiens

193

CKAP4

10970

Proximity Label-MS

Homo sapiens

194

ARSK

Affinity Capture-MS

Homo sapiens

195

HS6ST2

90161

Affinity Capture-MS

Homo sapiens

196

SEMA4B

Affinity Capture-MS

Homo sapiens

197

CNTNAP3B

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which DNAJB9 is involved

Pathway	Evidence	Source
Cellular responses to stimuli	TAS	Reactome
Cellular responses to stress	TAS	Reactome
IRE1alpha activates chaperones	TAS	Reactome
Unfolded Protein Response (UPR)	TAS	Reactome
XBP1(S) activates chaperone genes	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here