Gene description for DNAJB9
Gene name DnaJ (Hsp40) homolog, subfamily B, member 9
Gene symbol DNAJB9
Other names/aliases ERdj4
MDG-1
MDG1
MST049
MSTP049
Species Homo sapiens
 Database cross references - DNAJB9
ExoCarta ExoCarta_4189
Vesiclepedia VP_4189
Entrez Gene 4189
HGNC 6968
MIM 602634
UniProt Q9UBS3  
 DNAJB9 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Saliva 19199708    
 Gene ontology annotations for DNAJB9
Molecular Function
    misfolded protein binding GO:0051787 IDA
    protein binding GO:0005515 IPI
Biological Process
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 IMP
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    cellular protein metabolic process GO:0044267 TAS
    IRE1-mediated unfolded protein response GO:0036498 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    cytoplasm GO:0005737 ISS
    extracellular exosome GO:0070062 IDA
    nucleolus GO:0005730 ISS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 IEA
 Experiment description of studies that identified DNAJB9 in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 66
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix|CD63|CD81
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for DNAJB9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC16A8  
Two-hybrid Homo sapiens
2 FSCN1 6624
Two-hybrid Homo sapiens
3 MYL6 4637
Two-hybrid Homo sapiens
4 PLK1 5347
Two-hybrid Homo sapiens
5 DYNLL1 8655
Two-hybrid Homo sapiens
6 TCERG1 10915
Affinity Capture-MS Homo sapiens
7 TCEA2  
Two-hybrid Homo sapiens
8 RFK 55312
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJB9 is involved
PathwayEvidenceSource
XBP1(S) activates chaperone genes TAS Reactome





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