Gene description for P3H4
Gene name prolyl 3-hydroxylase family member 4 (non-enzymatic)
Gene symbol P3H4
Other names/aliases LEPREL4
NO55
NOL55
SC65
Species Homo sapiens
 Database cross references - P3H4
ExoCarta ExoCarta_10609
Vesiclepedia VP_10609
Entrez Gene 10609
HGNC 16946
UniProt Q92791  
 P3H4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for P3H4
Molecular Function
    collagen binding GO:0005518 IBA
Biological Process
    synaptonemal complex assembly GO:0007130 TAS
    peptidyl-lysine hydroxylation GO:0017185 ISS
    collagen fibril organization GO:0030199 IBA
    collagen fibril organization GO:0030199 ISS
    collagen biosynthetic process GO:0032964 ISS
    bone remodeling GO:0046849 ISS
Subcellular Localization
    condensed nuclear chromosome GO:0000794 TAS
    synaptonemal complex GO:0000795 TAS
    nucleolus GO:0005730 TAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    catalytic complex GO:1902494 IEA
 Experiment description of studies that identified P3H4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for P3H4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 P3H1 64175
Affinity Capture-MS Homo sapiens
3 SERPINA6  
Affinity Capture-MS Homo sapiens
4 CANX 821
Affinity Capture-MS Homo sapiens
5 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 EPDR1 54749
Affinity Capture-MS Homo sapiens
7 NAAA  
Affinity Capture-MS Homo sapiens
8 FOXA3  
Affinity Capture-MS Homo sapiens
9 C1QTNF9B  
Affinity Capture-MS Homo sapiens
10 ECEL1  
Affinity Capture-MS Homo sapiens
11 TMEM87A 25963
Affinity Capture-MS Homo sapiens
12 CPSF6 11052
Affinity Capture-MS Homo sapiens
13 LIPH 200879
Affinity Capture-MS Homo sapiens
14 SLC2A4  
Affinity Capture-MS Homo sapiens
15 TAZ  
Affinity Capture-MS Homo sapiens
16 CHST8 64377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GGH 8836
Affinity Capture-MS Homo sapiens
18 NCR3  
Affinity Capture-MS Homo sapiens
19 DHH  
Affinity Capture-MS Homo sapiens
20 CFC1  
Affinity Capture-MS Homo sapiens
21 PDGFRA 5156
Affinity Capture-MS Homo sapiens
22 FASLG 356
Affinity Capture-MS Homo sapiens
23 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 COLEC10 10584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PI15  
Affinity Capture-MS Homo sapiens
26 PTEN 5728
Affinity Capture-MS Homo sapiens
27 RLN1  
Affinity Capture-MS Homo sapiens
28 CBLN4  
Affinity Capture-MS Homo sapiens
29 CUL3 8452
Affinity Capture-MS Homo sapiens
30 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 LYZL1  
Affinity Capture-MS Homo sapiens
32 FCN3 8547
Affinity Capture-MS Homo sapiens
33 KNG1 3827
Affinity Capture-MS Homo sapiens
34 OS9 10956
Affinity Capture-MS Homo sapiens
35 IL5RA  
Affinity Capture-MS Homo sapiens
36 C1QTNF8  
Affinity Capture-MS Homo sapiens
37 EPHA7 2045
Affinity Capture-MS Homo sapiens
38 APEX1 328
Affinity Capture-RNA Homo sapiens
39 C1QB 713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SIRPD  
Affinity Capture-MS Homo sapiens
41 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SDF2L1 23753
Affinity Capture-MS Homo sapiens
43 FIBIN  
Affinity Capture-MS Homo sapiens
44 OGT 8473
Reconstituted Complex Homo sapiens
45 CRISP2  
Affinity Capture-MS Homo sapiens
46 ADAMTS4  
Affinity Capture-MS Homo sapiens
47 ALPP 250
Affinity Capture-MS Homo sapiens
48 DNAJB9 4189
Affinity Capture-MS Homo sapiens
49 COLQ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 BRD1 23774
Affinity Capture-MS Homo sapiens
51 CDHR4  
Affinity Capture-MS Homo sapiens
52 YWHAQ 10971
Affinity Capture-MS Homo sapiens
53 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
54 EDN3  
Affinity Capture-MS Homo sapiens
55 OLFM4 10562
Affinity Capture-MS Homo sapiens
56 MFAP4 4239
Affinity Capture-MS Homo sapiens
57 HRG 3273
Affinity Capture-MS Homo sapiens
58 RTN4R 65078
Affinity Capture-MS Homo sapiens
59 CHSY3 337876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ALX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 LIPG 9388
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which P3H4 is involved
No pathways found





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