Gene description for HRG
Gene name histidine-rich glycoprotein
Gene symbol HRG
Other names/aliases HPRG
HRGP
THPH11
Species Homo sapiens
 Database cross references - HRG
ExoCarta ExoCarta_3273
Vesiclepedia VP_3273
Entrez Gene 3273
HGNC 5181
MIM 142640
UniProt P04196  
 HRG identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for HRG
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    cysteine-type endopeptidase inhibitor activity GO:0004869 IEA
    signaling receptor binding GO:0005102 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IBA
    heparin binding GO:0008201 IDA
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
    immunoglobulin binding GO:0019865 IDA
    heme binding GO:0020037 IDA
    heparan sulfate proteoglycan binding GO:0043395 IDA
    metal ion binding GO:0046872 IDA
Biological Process
    angiogenesis GO:0001525 IEA
    positive regulation of immune response to tumor cell GO:0002839 IBA
    positive regulation of immune response to tumor cell GO:0002839 IDA
    chemotaxis GO:0006935 IEA
    negative regulation of cell adhesion GO:0007162 IDA
    negative regulation of cell population proliferation GO:0008285 IDA
    regulation of gene expression GO:0010468 IDA
    regulation of platelet activation GO:0010543 IBA
    regulation of platelet activation GO:0010543 ISS
    negative regulation of lamellipodium assembly GO:0010593 IDA
    negative regulation of angiogenesis GO:0016525 IDA
    platelet activation GO:0030168 IDA
    regulation of blood coagulation GO:0030193 IDA
    negative regulation of cell growth GO:0030308 IDA
    regulation of actin cytoskeleton organization GO:0032956 IDA
    negative regulation of cell adhesion mediated by integrin GO:0033629 IDA
    fibrinolysis GO:0042730 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    regulation of protein-containing complex assembly GO:0043254 IDA
    negative regulation of blood vessel endothelial cell migration GO:0043537 ISS
    regulation of peptidyl-tyrosine phosphorylation GO:0050730 IDA
    defense response to fungus GO:0050832 IDA
    cytolysis by host of symbiont cells GO:0051838 IDA
    positive regulation of focal adhesion assembly GO:0051894 IDA
    negative regulation of fibrinolysis GO:0051918 IBA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IDA
    negative regulation of vascular endothelial growth factor signaling pathway GO:1900747 IDA
    positive regulation of blood vessel remodeling GO:2000504 IDA
    negative regulation of endothelial cell chemotaxis GO:2001027 IDA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 IBA
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    platelet alpha granule lumen GO:0031093 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified HRG in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HRG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 CYSRT1 375791
Two-hybrid Homo sapiens
3 FBN1 2200
Affinity Capture-MS Homo sapiens
4 TNFRSF1A 7132
Two-hybrid Homo sapiens
5 RHBDD1 84236
Affinity Capture-MS Homo sapiens
6 RABAC1 10567
Two-hybrid Homo sapiens
7 GRN 2896
Affinity Capture-MS Homo sapiens
8 P4HA1 5033
Affinity Capture-MS Homo sapiens
9 P3H4 10609
Affinity Capture-MS Homo sapiens
10 FBN2 2201
Affinity Capture-MS Homo sapiens
11 AGPAT1 10554
Affinity Capture-MS Homo sapiens
12 JADE3  
Affinity Capture-MS Homo sapiens
13 MED30  
Affinity Capture-MS Homo sapiens
14 SERPING1 710
Affinity Capture-MS Homo sapiens
15 JADE1  
Affinity Capture-MS Homo sapiens
16 BRPF1  
Affinity Capture-MS Homo sapiens
17 GNG8  
Affinity Capture-MS Homo sapiens
18 ING4  
Affinity Capture-MS Homo sapiens
19 PLSCR1 5359
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
20 LTBP4 8425
Affinity Capture-MS Homo sapiens
21 RCHY1  
Two-hybrid Homo sapiens
22 IMPACT 55364
Affinity Capture-MS Homo sapiens
23 FGA 2243
Reconstituted Complex Homo sapiens
24 SPRY1 10252
Two-hybrid Homo sapiens
25 FN1 2335
Two-hybrid Homo sapiens
26 HIST1H2BO 8348
Affinity Capture-MS Homo sapiens
27 P3H3 10536
Affinity Capture-MS Homo sapiens
28 DPPA4  
Affinity Capture-MS Homo sapiens
29 MBNL1 4154
Affinity Capture-MS Homo sapiens
30 ABTB2 25841
Affinity Capture-MS Homo sapiens
31 RCCD1  
Affinity Capture-MS Homo sapiens
32 MYC  
Affinity Capture-MS Homo sapiens
33 KLK10 5655
Affinity Capture-MS Homo sapiens
34 SERPINC1 462
Affinity Capture-MS Homo sapiens
35 TRIM21 6737
Affinity Capture-MS Homo sapiens
36 SARAF  
Affinity Capture-MS Homo sapiens
37 CHRD  
Two-hybrid Homo sapiens
38 CRTAP 10491
Affinity Capture-MS Homo sapiens
39 C3 718
Affinity Capture-MS Homo sapiens
40 UBE2U  
Affinity Capture-MS Homo sapiens
41 PCGF1 84759
Affinity Capture-MS Homo sapiens
42 RGS20 8601
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
43 KAT6A  
Affinity Capture-MS Homo sapiens
44 PPP1R7 5510
Affinity Capture-MS Homo sapiens
45 POU5F1  
Affinity Capture-MS Homo sapiens
46 BRPF3  
Affinity Capture-MS Homo sapiens
47 UTY  
Affinity Capture-MS Homo sapiens
48 NANOG  
Affinity Capture-MS Homo sapiens
49 CCR1  
Affinity Capture-MS Homo sapiens
50 CUL4A 8451
Affinity Capture-MS Homo sapiens
51 FBLN5 10516
Affinity Capture-MS Homo sapiens
52 PHF11 51131
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here