Gene description for IMPACT
Gene name impact RWD domain protein
Gene symbol IMPACT
Other names/aliases RWDD5
Species Homo sapiens
 Database cross references - IMPACT
ExoCarta ExoCarta_55364
Vesiclepedia VP_55364
Entrez Gene 55364
HGNC 20387
MIM 615319
UniProt Q9P2X3  
 IMPACT identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IMPACT
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    ribosome binding GO:0043022 IEA
    protein sequestering activity GO:0140311 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    regulation of translational initiation GO:0006446 IBA
    cellular response to amino acid starvation GO:0034198 ISS
    intracellular signal transduction GO:0035556 ISS
    positive regulation of neuron differentiation GO:0045666 ISS
    GCN2-mediated signaling GO:0140469 IBA
    GCN2-mediated signaling GO:0140469 ISS
    neuron projection extension GO:1990138 ISS
    regulation of cytoplasmic translational initiation in response to stress GO:1990611 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified IMPACT in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IMPACT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX4  
Affinity Capture-MS Homo sapiens
2 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
3 REEP5 7905
Affinity Capture-MS Homo sapiens
4 HAP1  
Affinity Capture-MS Homo sapiens
5 LLGL2 3993
Affinity Capture-MS Homo sapiens
6 CA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CRHBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TMEM69  
Affinity Capture-MS Homo sapiens
9 CDC73  
Affinity Capture-MS Homo sapiens
10 KIAA1107  
Affinity Capture-MS Homo sapiens
11 SMURF1 57154
Biochemical Activity Homo sapiens
12 Dync1li1 235661
Affinity Capture-MS Mus musculus
13 RRP8  
Affinity Capture-MS Homo sapiens
14 FAS 355
Proximity Label-MS Homo sapiens
15 EPB41L5 57669
Affinity Capture-MS Homo sapiens
16 Poc1a  
Affinity Capture-MS Mus musculus
17 SURF6  
Affinity Capture-MS Homo sapiens
18 HRG 3273
Affinity Capture-MS Homo sapiens
19 SGOL1  
Affinity Capture-MS Homo sapiens
20 FANCI 55215
Affinity Capture-MS Homo sapiens
21 Shoc2  
Affinity Capture-MS Mus musculus
22 MAP3K1 4214
Biochemical Activity Homo sapiens
23 KIF7 374654
Affinity Capture-MS Homo sapiens
24 Vps4b 20479
Affinity Capture-MS Mus musculus
25 C10orf88  
Affinity Capture-MS Homo sapiens
26 QSOX2 169714
Affinity Capture-MS Homo sapiens
27 SERPINF1 5176
Two-hybrid Homo sapiens
28 ETS2  
Affinity Capture-MS Homo sapiens
29 Hnrnpa3 229279
Affinity Capture-MS Mus musculus
30 Pafah1b1 18472
Affinity Capture-MS Mus musculus
31 CRKL 1399
Affinity Capture-MS Homo sapiens
32 PPP1R21 129285
Affinity Capture-MS Homo sapiens
33 TGFA  
Affinity Capture-MS Homo sapiens
34 Naa10  
Affinity Capture-MS Mus musculus
35 Bub1b 12236
Affinity Capture-MS Mus musculus
36 Chmp6  
Affinity Capture-MS Mus musculus
37 RAB5C 5878
Affinity Capture-MS Homo sapiens
38 Bub1  
Affinity Capture-MS Mus musculus
39 Pdlim5 56376
Affinity Capture-MS Mus musculus
40 KIF20B  
Affinity Capture-MS Homo sapiens
41 LRRC3B  
Affinity Capture-MS Homo sapiens
42 FAM133A 286499
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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