Gene description for Bub1b
Gene name budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae)
Gene symbol Bub1b
Other names/aliases AU045529
BUBR1
Species Mus musculus
 Database cross references - Bub1b
ExoCarta ExoCarta_12236
Vesiclepedia VP_12236
Entrez Gene 12236
UniProt Q9Z1S0  
 Bub1b identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Bub1b
Molecular Function
    protein kinase activity GO:0004672 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    apoptotic process GO:0006915 IEA
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 IGI
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 ISO
    mitotic spindle assembly checkpoint signaling GO:0007094 ISS
    mitotic spindle assembly checkpoint signaling GO:0007094 NAS
    cell division GO:0051301 IEA
    meiotic sister chromatid cohesion, centromeric GO:0051754 IBA
    protein localization to chromosome, centromeric region GO:0071459 IMP
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IDA
    kinetochore GO:0000776 IMP
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    outer kinetochore GO:0000940 IDA
    outer kinetochore GO:0000940 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    spindle GO:0005819 IEA
    spindle GO:0005819 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    mitotic checkpoint complex GO:0033597 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Bub1b in exosomes
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Bub1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC7 51434
Affinity Capture-MS Homo sapiens
2 DSN1 79980
Affinity Capture-MS Homo sapiens
3 MIS12  
Affinity Capture-MS Homo sapiens
4 Incenp  
Co-fractionation Mus musculus
5 CDC26 246184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NUF2  
Affinity Capture-MS Homo sapiens
7 ANAPC16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CDC20 991
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ANAPC13 25847
Affinity Capture-MS Homo sapiens
10 Spag5  
Co-fractionation Mus musculus
11 Nup85  
Co-fractionation Mus musculus
12 ZWINT  
Affinity Capture-MS Homo sapiens
13 BUB1  
Affinity Capture-MS Homo sapiens
14 Zfp276 57247
Co-fractionation Mus musculus
15 HECTD4 283450
Affinity Capture-MS Homo sapiens
16 GOLIM4 27333
Affinity Capture-MS Homo sapiens
17 ASAH1 427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ANAPC5 51433
Affinity Capture-MS Homo sapiens
19 NSL1  
Affinity Capture-MS Homo sapiens
20 CDCA3 83461
Affinity Capture-MS Homo sapiens
21 CDC16 8881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Cenpi  
Co-fractionation Mus musculus
23 SPC24 147841
Affinity Capture-MS Homo sapiens
24 SEC63 11231
Affinity Capture-MS Homo sapiens
25 BUB1B  
Affinity Capture-MS Homo sapiens
26 Nup43  
Co-fractionation Mus musculus
27 CDC23 8697
Affinity Capture-MS Homo sapiens
28 ANAPC1 64682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CWF19L2  
Affinity Capture-MS Homo sapiens
30 Slc25a24  
Co-fractionation Mus musculus
31 IMPACT 55364
Affinity Capture-MS Homo sapiens
32 Tex14  
Co-fractionation Mus musculus
33 BUB3 9184
Affinity Capture-MS Homo sapiens
34 Cdc20  
Affinity Capture-Western Mus musculus
35 ANAPC2 29882
Affinity Capture-MS Homo sapiens
36 SPC25 57405
Affinity Capture-MS Homo sapiens
37 FZR1  
Affinity Capture-MS Homo sapiens
38 UBR5 51366
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 Pbrm1  
Co-fractionation Mus musculus
40 STX3 6809
Affinity Capture-MS Homo sapiens
41 IARS2 55699
Affinity Capture-MS Homo sapiens
42 ANAPC10  
Affinity Capture-MS Homo sapiens
43 RABL6 55684
Affinity Capture-MS Homo sapiens
44 IGF2R 3482
Affinity Capture-MS Homo sapiens
45 ZMAT3  
Affinity Capture-MS Homo sapiens
46 CASC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 NDC80 10403
Affinity Capture-MS Homo sapiens
48 Hdac11  
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
49 REPS2  
Affinity Capture-MS Homo sapiens
50 Lmo2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
51 GOLM1 51280
Affinity Capture-MS Homo sapiens
52 Mis18bp1  
Co-fractionation Mus musculus
53 MAD2L1 4085
Affinity Capture-MS Homo sapiens
54 CDC27 996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 MROH1 727957
Affinity Capture-MS Homo sapiens
56 ANAPC4 29945
Affinity Capture-MS Homo sapiens
57 Seh1l 72124
Co-fractionation Mus musculus
58 Zwilch  
Co-fractionation Mus musculus
59 Apc  
Phenotypic Enhancement Mus musculus
View the network image/svg+xml
 Pathways in which Bub1b is involved
PathwayEvidenceSource
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
APC-Cdc20 mediated degradation of Nek2A IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex IEA Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components IEA Reactome
M Phase IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Regulation of APC/C activators between G1/S and early anaphase IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome





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