Gene description for Bub1b
Gene name budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae)
Gene symbol Bub1b
Other names/aliases AU045529
BUBR1
Species Mus musculus
 Database cross references - Bub1b
ExoCarta ExoCarta_12236
Entrez Gene 12236
UniProt Q9Z1S0  
 Bub1b identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Bub1b
Molecular Function
    transferase activity GO:0016740 IEA
    kinase activity GO:0016301 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    ATP binding GO:0005524 IEA
    protein kinase activity GO:0004672 IEA
    protein serine/threonine kinase activity GO:0004674 IEA
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
Biological Process
    cell cycle GO:0007049 IEA
    cell division GO:0051301 IEA
    protein localization to kinetochore GO:0034501 ISO
    apoptotic process GO:0006915 IEA
    phosphorylation GO:0016310 IEA
    mitotic nuclear division GO:0007067 IEA
    protein localization to chromosome, centromeric region GO:0071459 IMP
    metaphase/anaphase transition of mitotic cell cycle GO:0007091 IGI
    protein phosphorylation GO:0006468 IEA
Subcellular Localization
    condensed chromosome outer kinetochore GO:0000940 ISO
    chromosome, centromeric region GO:0000775 IEA
    condensed nuclear chromosome kinetochore GO:0000778 IDA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    kinetochore GO:0000776 ISO
    condensed chromosome kinetochore GO:0000777 ISO
    chromosome GO:0005694 IEA
 Experiment description of studies that identified Bub1b in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Bub1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here