Gene description for Seh1l
Gene name SEH1-like (S. cerevisiae
Gene symbol Seh1l
Other names/aliases 2610007A16Rik
AW540070
SEC13L
SEH1A
SEH1B
Seh1
Species Mus musculus
 Database cross references - Seh1l
ExoCarta ExoCarta_72124
Vesiclepedia VP_72124
Entrez Gene 72124
UniProt Q8R2U0  
 Seh1l identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Seh1l
Molecular Function
    structural molecule activity GO:0005198 IEA
Biological Process
    cytokine production involved in inflammatory response GO:0002534 IMP
    nucleocytoplasmic transport GO:0006913 NAS
    nuclear pore organization GO:0006999 ISO
    nuclear pore organization GO:0006999 ISS
    mitotic metaphase chromosome alignment GO:0007080 ISO
    mitotic metaphase chromosome alignment GO:0007080 ISS
    protein transport GO:0015031 IEA
    protein-containing complex localization GO:0031503 IEA
    protein-containing complex localization GO:0031503 ISO
    cellular response to nutrient levels GO:0031669 ISO
    cellular response to nutrient levels GO:0031669 ISS
    cellular response to amino acid starvation GO:0034198 IBA
    cellular response to amino acid starvation GO:0034198 IEA
    cellular response to amino acid starvation GO:0034198 ISO
    defense response to Gram-positive bacterium GO:0050830 IMP
    mRNA transport GO:0051028 IEA
    cell division GO:0051301 IEA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 ISO
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 ISS
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    nuclear envelope GO:0005635 ISO
    nuclear pore GO:0005643 NAS
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    nuclear pore outer ring GO:0031080 IBA
    nuclear pore outer ring GO:0031080 ISO
    nuclear pore outer ring GO:0031080 ISS
    Seh1-associated complex GO:0035859 IBA
    GATOR2 complex GO:0061700 ISO
    GATOR2 complex GO:0061700 ISS
 Experiment description of studies that identified Seh1l in exosomes
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Seh1l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cenpi  
Co-fractionation Mus musculus
2 Clasp1  
Co-fractionation Mus musculus
3 Mios  
Co-fractionation Mus musculus
4 Nup35  
Co-fractionation Mus musculus
5 Spag5  
Co-fractionation Mus musculus
6 Nup85  
Co-fractionation Mus musculus
7 Wdr59  
Co-fractionation Mus musculus
8 Zfp276 57247
Co-fractionation Mus musculus
9 Trappc12  
Co-fractionation Mus musculus
10 Zw10  
Co-fractionation Mus musculus
11 Sec13 110379
Co-fractionation Mus musculus
12 Szt2  
Co-fractionation Mus musculus
13 Ckap5 75786
Co-fractionation Mus musculus
14 Aaas  
Co-fractionation Mus musculus
15 Nupl2  
Co-fractionation Mus musculus
16 Nup93  
Co-fractionation Mus musculus
17 Pdia5  
Co-fractionation Mus musculus
18 Nup214  
Co-fractionation Mus musculus
19 Nup62  
Co-fractionation Mus musculus
20 Nup107  
Co-fractionation Mus musculus
21 Depdc5  
Co-fractionation Mus musculus
22 Nup133  
Co-fractionation Mus musculus
23 Bub1b 12236
Co-fractionation Mus musculus
24 Nup43  
Co-fractionation Mus musculus
25 Sgol1  
Co-fractionation Mus musculus
26 Atg16l1  
Affinity Capture-MS Mus musculus
27 Zwilch  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Seh1l is involved
PathwayEvidenceSource
Amino acids regulate mTORC1 IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
M Phase IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome
Metabolism of non-coding RNA IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Prophase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown IEA Reactome
Nuclear Pore Complex (NPC) Disassembly IEA Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
snRNP Assembly IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins IEA Reactome
SUMOylation of DNA damage response and repair proteins IEA Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of RNA binding proteins IEA Reactome
SUMOylation of SUMOylation proteins IEA Reactome
SUMOylation of ubiquitinylation proteins IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript IEA Reactome
Transport of Mature mRNA Derived from an Intronless Transcript IEA Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts IEA Reactome
Transport of Mature Transcript to Cytoplasm IEA Reactome
Transport of the SLBP Dependant Mature mRNA IEA Reactome
Transport of the SLBP independent Mature mRNA IEA Reactome





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