Gene description for ANAPC5
Gene name anaphase promoting complex subunit 5
Gene symbol ANAPC5
Other names/aliases APC5
Species Homo sapiens
 Database cross references - ANAPC5
ExoCarta ExoCarta_51433
Entrez Gene 51433
HGNC 15713
MIM 606948
UniProt Q9UJX4  
 ANAPC5 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ANAPC5
Molecular Function
    protein phosphatase binding GO:0019903 IPI
Biological Process
    protein K11-linked ubiquitination GO:0070979 IDA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    mitotic nuclear division GO:0007067 IEA
    mitotic spindle assembly checkpoint GO:0007094 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    cell division GO:0051301 IEA
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    mitotic cell cycle GO:0000278 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    anaphase-promoting complex GO:0005680 IDA
 Experiment description of studies that identified ANAPC5 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ANAPC5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
2 CDC20  
Reconstituted Complex Homo sapiens
3 APC2  
Affinity Capture-Western Homo sapiens
4 ANAPC2 29882
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
5 ANAPC10  
Invivo Homo sapiens
6 FZR1  
Reconstituted Complex Homo sapiens
7 CDC16 8881
Invivo Homo sapiens
8 CDC23 8697
Invivo Homo sapiens
9 ANAPC1 64682
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Invivo Homo sapiens
10 ANAPC11  
Invivo Homo sapiens
11 ZBTB16  
Two-hybrid Homo sapiens
12 ANAPC7 51434
Reconstituted Complex Homo sapiens
13 PABPC1 26986
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 CDC27 996
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here