Gene description for VCPIP1
Gene name valosin containing protein (p97)/p47 complex interacting protein 1
Gene symbol VCPIP1
Other names/aliases DUBA3
VCIP135
Species Homo sapiens
 Database cross references - VCPIP1
ExoCarta ExoCarta_80124
Vesiclepedia VP_80124
Entrez Gene 80124
HGNC 30897
MIM 611745
UniProt Q96JH7  
 VCPIP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for VCPIP1
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 ISS
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    DNA damage response GO:0006974 IDA
    endoplasmic reticulum membrane fusion GO:0016320 IBA
    protein ubiquitination GO:0016567 ISS
    protein deubiquitination GO:0016579 IDA
    protein K11-linked deubiquitination GO:0035871 IDA
    protein K48-linked deubiquitination GO:0071108 IDA
    Golgi reassembly GO:0090168 IBA
    protein-DNA covalent cross-linking repair GO:0106300 IBA
    protein-DNA covalent cross-linking repair GO:0106300 IDA
    regulation of protein localization to chromatin GO:1905634 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi stack GO:0005795 IEA
 Experiment description of studies that identified VCPIP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VCPIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLFN11 91607
Affinity Capture-MS Homo sapiens
2 KRT37 8688
Affinity Capture-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
5 NPRL2  
Affinity Capture-MS Homo sapiens
6 SNX24  
Affinity Capture-MS Homo sapiens
7 HSPA6 3310
Co-fractionation Homo sapiens
8 JMJD4  
Affinity Capture-MS Homo sapiens
9 RPA2 6118
Proximity Label-MS Homo sapiens
10 ANAPC5 51433
Affinity Capture-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
12 UBXN2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 UFD1L 7353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GSK3A 2931
Two-hybrid Homo sapiens
15 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
16 S100A4 6275
Affinity Capture-MS Homo sapiens
17 INSRR 3645
Affinity Capture-MS Homo sapiens
18 D2HGDH 728294
Affinity Capture-MS Homo sapiens
19 RFXANK  
Affinity Capture-MS Homo sapiens
20 ABCC12  
Affinity Capture-MS Homo sapiens
21 TSEN15 116461
Affinity Capture-MS Homo sapiens
22 PLD2 5338
Affinity Capture-MS Homo sapiens
23 CAMK2B 816
Affinity Capture-MS Homo sapiens
24 SVIP 258010
Affinity Capture-MS Homo sapiens
25 FIGNL1 63979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 FBXO7 25793
Affinity Capture-MS Homo sapiens
27 NSFL1C 55968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NPAS1  
Affinity Capture-MS Homo sapiens
29 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NINL  
Proximity Label-MS Homo sapiens
31 PXMP2  
Proximity Label-MS Homo sapiens
32 FAM96A  
Affinity Capture-MS Homo sapiens
33 S100A2 6273
Affinity Capture-MS Homo sapiens
34 RNF41  
Affinity Capture-MS Homo sapiens
35 GSTM3 2947
Affinity Capture-MS Homo sapiens
36 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
37 PLBD1 79887
Affinity Capture-MS Homo sapiens
38 SPG11 80208
Affinity Capture-MS Homo sapiens
39 CLTCL1 8218
Affinity Capture-MS Homo sapiens
40 WAC  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
41 CDH1 999
Proximity Label-MS Homo sapiens
42 UBXN8  
Affinity Capture-MS Homo sapiens
43 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
44 NT5C 30833
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 TTLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PPARD  
Affinity Capture-MS Homo sapiens
48 F12  
Affinity Capture-MS Homo sapiens
49 PRMT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ACTB 60
Proximity Label-MS Homo sapiens
51 TUBA1B 10376
Affinity Capture-MS Homo sapiens
52 FAF1 11124
Affinity Capture-MS Homo sapiens
53 STX5 6811
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 VAV2 7410
Two-hybrid Homo sapiens
55 PEX11A  
Affinity Capture-MS Homo sapiens
56 KIF20B  
Affinity Capture-MS Homo sapiens
57 UBXN6 80700
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 USP32 84669
Affinity Capture-MS Homo sapiens
59 PSG11  
Affinity Capture-MS Homo sapiens
60 PHLPP1  
Proximity Label-MS Homo sapiens
61 GAN 8139
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 DCTN3 11258
Affinity Capture-MS Homo sapiens
63 DGKG  
Affinity Capture-MS Homo sapiens
64 MAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 NR0B2  
Affinity Capture-MS Homo sapiens
66 UBXN7 26043
Affinity Capture-MS Homo sapiens
67 GGH 8836
Affinity Capture-MS Homo sapiens
68 CTNNB1 1499
Proximity Label-MS Homo sapiens
69 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
70 VPS16 64601
Affinity Capture-MS Homo sapiens
71 METTL21B  
Affinity Capture-MS Homo sapiens
72 CNKSR3 154043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 TBCD 6904
Affinity Capture-MS Homo sapiens
74 NXF1 10482
Affinity Capture-RNA Homo sapiens
75 NPLOC4 55666
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FANCA  
Affinity Capture-MS Homo sapiens
77 DND1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 RPA3 6119
Proximity Label-MS Homo sapiens
79 SMAD9  
Two-hybrid Homo sapiens
80 ARL4D  
Affinity Capture-MS Homo sapiens
81 FCHSD2  
Two-hybrid Homo sapiens
82 AP5B1 91056
Affinity Capture-MS Homo sapiens
83 N4BP2L2 10443
Affinity Capture-MS Homo sapiens
84 BICD1 636
Proximity Label-MS Homo sapiens
85 MFN2 9927
Proximity Label-MS Homo sapiens
86 CDC20B  
Affinity Capture-MS Homo sapiens
87 UBXN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 UBXN1 51035
Affinity Capture-MS Homo sapiens
89 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 RNF43  
Proximity Label-MS Homo sapiens
91 DSCC1  
Affinity Capture-MS Homo sapiens
92 ASPSCR1 79058
Affinity Capture-MS Homo sapiens
93 MCL1 4170
Affinity Capture-MS Homo sapiens
94 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
95 UBXN11  
Affinity Capture-MS Homo sapiens
96 VPS33A 65082
Affinity Capture-MS Homo sapiens
97 PFN1 5216
Proximity Label-MS Homo sapiens
98 LARP7 51574
Co-fractionation Homo sapiens
99 DDX11 1663
Affinity Capture-MS Homo sapiens
100 TRIM37  
Proximity Label-MS Homo sapiens
101 DHFR 1719
Affinity Capture-MS Homo sapiens
102 MOV10 4343
Affinity Capture-RNA Homo sapiens
103 KRT38 8687
Affinity Capture-MS Homo sapiens
104 C16orf72 29035
Affinity Capture-MS Homo sapiens
105 EID3  
Affinity Capture-MS Homo sapiens
106 OLFM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 KCTD14 65987
Affinity Capture-MS Homo sapiens
108 NFKBIB  
Affinity Capture-MS Homo sapiens
109 HRASLS5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 ZNF318 24149
Affinity Capture-MS Homo sapiens
111 USP9Y 8287
Affinity Capture-MS Homo sapiens
112 LRCH3 84859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 NIN 51199
Proximity Label-MS Homo sapiens
114 KDM5C  
Affinity Capture-MS Homo sapiens
115 ATP13A1 57130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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