Gene description for DDX11
Gene name DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
Gene symbol DDX11
Other names/aliases CHL1
CHLR1
KRG2
WABS
Species Homo sapiens
 Database cross references - DDX11
ExoCarta ExoCarta_1663
Entrez Gene 1663
HGNC 2736
MIM 601150
UniProt Q96FC9  
 DDX11 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for DDX11
Molecular Function
    helicase activity GO:0004386 IDA
    ATP binding GO:0005524 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    DNA-dependent ATPase activity GO:0008094 IDA
    single-stranded DNA binding GO:0003697 IDA
    metal ion binding GO:0046872 IEA
    double-stranded DNA binding GO:0003690 IDA
    RNA binding GO:0003723 IEA
    ATP-dependent DNA helicase activity GO:0004003 IEA
    protein binding GO:0005515 IPI
Biological Process
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
    viral process GO:0016032 IEA
    cellular protein metabolic process GO:0044267 TAS
    sister chromatid cohesion GO:0007062 IDA
    DNA duplex unwinding GO:0032508 IEA
    nucleobase-containing compound metabolic process GO:0006139 IEA
    IRE1-mediated unfolded protein response GO:0036498 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
    nuclear chromatin GO:0000790 IDA
    spindle pole GO:0000922 IDA
    nucleolus GO:0005730 IDA
    midbody GO:0030496 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DDX11 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for DDX11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYST2  
Two-hybrid Homo sapiens
2 PFN2 5217
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DDX11 is involved
PathwayEvidenceSource
XBP1(S) activates chaperone genes TAS Reactome





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