Gene description for CDH23
Gene name cadherin-related 23
Gene symbol CDH23
Other names/aliases CDHR23
USH1D
Species Homo sapiens
 Database cross references - CDH23
ExoCarta ExoCarta_64072
Vesiclepedia VP_64072
Entrez Gene 64072
HGNC 13733
MIM 605516
UniProt Q9H251  
 CDH23 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CDH23
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IBA
    cadherin binding GO:0045296 IBA
Biological Process
    calcium ion transport GO:0006816 IMP
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    visual perception GO:0007601 IEA
    sensory perception of sound GO:0007605 IMP
    sensory perception of sound GO:0007605 IMP
    locomotory behavior GO:0007626 IEA
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 NAS
    cell migration GO:0016477 IBA
    neuron projection development GO:0031175 IBA
    photoreceptor cell maintenance GO:0045494 IMP
    sensory perception of light stimulus GO:0050953 IMP
    equilibrioception GO:0050957 IMP
    regulation of cytosolic calcium ion concentration GO:0051480 IMP
    auditory receptor cell stereocilium organization GO:0060088 IEA
    cochlea development GO:0090102 IEA
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 IBA
Subcellular Localization
    photoreceptor inner segment GO:0001917 IEA
    centrosome GO:0005813 IEA
    membrane GO:0016020 NAS
    catenin complex GO:0016342 IBA
    stereocilium GO:0032420 ISS
    stereocilium tip GO:0032426 IEA
    kinocilium GO:0060091 IEA
    cochlear hair cell ribbon synapse GO:0098683 IEA
    photoreceptor ribbon synapse GO:0098684 IEA
 Experiment description of studies that identified CDH23 in exosomes
1
Experiment ID 254
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 259
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 260
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDH23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YEATS2  
Affinity Capture-MS Homo sapiens
2 UTP15 84135
Affinity Capture-MS Homo sapiens
3 USH1C  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 TADA1  
Affinity Capture-MS Homo sapiens
5 TGFB1 7040
Affinity Capture-MS Homo sapiens
6 SPECC1 92521
Affinity Capture-MS Homo sapiens
7 GTF2F1 2962
Affinity Capture-MS Homo sapiens
8 LAMC3  
Affinity Capture-MS Homo sapiens
9 ITGA5 3678
Affinity Capture-MS Homo sapiens
10 GDF11 10220
Affinity Capture-MS Homo sapiens
11 MAP7 9053
Affinity Capture-MS Homo sapiens
12 CNTNAP3  
Affinity Capture-MS Homo sapiens
13 MUM1  
Affinity Capture-MS Homo sapiens
14 DVL2 1856
Affinity Capture-MS Homo sapiens
15 FOXRED2  
Affinity Capture-MS Homo sapiens
16 TCTN1  
Affinity Capture-MS Homo sapiens
17 TAF5L  
Affinity Capture-MS Homo sapiens
18 MELK  
Affinity Capture-MS Homo sapiens
19 TADA3  
Affinity Capture-MS Homo sapiens
20 AGAP1  
Affinity Capture-MS Homo sapiens
21 GTPBP10  
Affinity Capture-MS Homo sapiens
22 NCKAP1 10787
Affinity Capture-MS Homo sapiens
23 RPS23 6228
Affinity Capture-MS Homo sapiens
24 CHST7  
Affinity Capture-MS Homo sapiens
25 FLYWCH2  
Affinity Capture-MS Homo sapiens
26 COX16  
Affinity Capture-MS Homo sapiens
27 CDC6  
Affinity Capture-MS Homo sapiens
28 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
29 RPL23 9349
Affinity Capture-MS Homo sapiens
30 WASF1 8936
Affinity Capture-MS Homo sapiens
31 SAMD1  
Affinity Capture-MS Homo sapiens
32 PCSK5 5125
Affinity Capture-MS Homo sapiens
33 MAP3K7  
Affinity Capture-MS Homo sapiens
34 MAP7D3 79649
Affinity Capture-MS Homo sapiens
35 CSRP2BP  
Affinity Capture-MS Homo sapiens
36 LRIF1  
Affinity Capture-MS Homo sapiens
37 FAM162A 26355
Affinity Capture-MS Homo sapiens
38 DDX11 1663
Affinity Capture-MS Homo sapiens
39 MBIP  
Affinity Capture-MS Homo sapiens
40 GHRH  
Affinity Capture-MS Homo sapiens
41 ERF  
Affinity Capture-MS Homo sapiens
42 SULF2 55959
Affinity Capture-MS Homo sapiens
43 LAMA3 3909
Affinity Capture-MS Homo sapiens
44 SERPINE2 5270
Affinity Capture-MS Homo sapiens
45 GLB1L 79411
Affinity Capture-MS Homo sapiens
46 TAF6L 10629
Affinity Capture-MS Homo sapiens
47 EMILIN3  
Affinity Capture-MS Homo sapiens
48 PTPMT1 114971
Affinity Capture-MS Homo sapiens
49 RPUSD3  
Affinity Capture-MS Homo sapiens
50 COCH 1690
Affinity Capture-MS Homo sapiens
51 CBX6  
Affinity Capture-MS Homo sapiens
52 GOPC 57120
Affinity Capture-MS Homo sapiens
53 ZNF518A 9849
Affinity Capture-MS Homo sapiens
54 LTBP1 4052
Affinity Capture-MS Homo sapiens
55 CYFIP1 23191
Affinity Capture-MS Homo sapiens
56 ABI1 10006
Affinity Capture-MS Homo sapiens
57 NT5C3A 51251
Affinity Capture-MS Homo sapiens
58 TIMP3 7078
Affinity Capture-MS Homo sapiens
59 MAP7D2 256714
Affinity Capture-MS Homo sapiens
60 PEAK1 79834
Affinity Capture-MS Homo sapiens
61 NXN 64359
Affinity Capture-MS Homo sapiens
62 PPME1 51400
Affinity Capture-MS Homo sapiens
63 TMTC4  
Affinity Capture-MS Homo sapiens
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