Gene description for GTF2F1
Gene name general transcription factor IIF, polypeptide 1, 74kDa
Gene symbol GTF2F1
Other names/aliases BTF4
RAP74
TF2F1
TFIIF
Species Homo sapiens
 Database cross references - GTF2F1
ExoCarta ExoCarta_2962
Entrez Gene 2962
HGNC 4652
MIM 189968
UniProt P35269  
 GTF2F1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for GTF2F1
Molecular Function
    phosphatase activator activity GO:0019211 IDA
    catalytic activity GO:0003824 IEA
    transcription coactivator activity GO:0003713 TAS
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    transcription factor binding GO:0008134 IPI
Biological Process
    RNA splicing GO:0008380 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    gene expression GO:0010467 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    positive regulation of transcription elongation from RNA polymerase II promoter GO:0032968 IEA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    response to virus GO:0009615 IEP
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    viral process GO:0016032 TAS
    positive regulation of viral transcription GO:0050434 TAS
    positive regulation of catalytic activity GO:0043085 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cell junction GO:0030054 IDA
    transcription factor TFIIF complex GO:0005674 TAS
 Experiment description of studies that identified GTF2F1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for GTF2F1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTN  
Two-hybrid Homo sapiens
2 FEZ1  
Two-hybrid Homo sapiens
3 CTDSP1 58190
Affinity Capture-MS Homo sapiens
4 GTF2E1  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
5 BRCA1 672
Co-purification Homo sapiens
6 GTF2F2  
Affinity Capture-MS Homo sapiens
7 TAF1  
Invivo Homo sapiens
Invitro Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
8 MSX2  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
9 POLR2A 5430
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
10 GTF2B 2959
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
11 TBP  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
12 JUN 3725
Reconstituted Complex Homo sapiens
13 GTF2A1  
Reconstituted Complex Homo sapiens
14 TAF11  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
15 GTF2H4  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
16 FOS 2353
Reconstituted Complex Homo sapiens
17 MYC  
Reconstituted Complex Homo sapiens
18 FCP1  
Reconstituted Complex Homo sapiens
19 GTF2E2  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
20 AHR 196
Reconstituted Complex Homo sapiens
21 ARNT 405
Reconstituted Complex Homo sapiens
22 SRF  
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
23 SURB7  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
24 AR 367
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
25 PSMC2 5701
Affinity Capture-MS Homo sapiens
26 CTDP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2F1 is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Transcription Initiation IEA Reactome
mRNA Capping TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Pausing and recovery of HIV elongation TAS Reactome
Pausing and recovery of Tat-mediated HIV elongation TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
Transcription of the HIV genome IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here