Gene description for KIF2C
Gene name kinesin family member 2C
Gene symbol KIF2C
Other names/aliases CT139
KNSL6
MCAK
Species Homo sapiens
 Database cross references - KIF2C
ExoCarta ExoCarta_11004
Vesiclepedia VP_11004
Entrez Gene 11004
HGNC 6393
MIM 604538
UniProt Q99661  
 KIF2C identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for KIF2C
Molecular Function
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    ATP hydrolysis activity GO:0016887 IBA
    centromeric DNA binding GO:0019237 TAS
    microtubule plus-end binding GO:0051010 IDA
Biological Process
    microtubule-based movement GO:0007018 IBA
    microtubule depolymerization GO:0007019 IDA
    microtubule depolymerization GO:0007019 IMP
    microtubule depolymerization GO:0007019 TAS
    mitotic metaphase chromosome alignment GO:0007080 IMP
    establishment or maintenance of microtubule cytoskeleton polarity GO:0030951 IMP
    cell division GO:0051301 IEA
    metaphase chromosome alignment GO:0051310 IMP
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IMP
    regulation of chromosome segregation GO:0051983 IMP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IEA
    postsynaptic cytoskeleton organization GO:0099188 IEA
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    kinetochore GO:0000776 IDA
    kinetochore GO:0000776 IDA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IBA
    spindle GO:0005819 IBA
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    cytoplasmic microtubule GO:0005881 ISS
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 HDA
    microtubule plus-end GO:0035371 IDA
    microtubule plus-end GO:0035371 TAS
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified KIF2C in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF2C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CENPB 1059
Affinity Capture-MS Homo sapiens
2 ZNF668  
Affinity Capture-MS Homo sapiens
3 GTF2F1 2962
Affinity Capture-MS Homo sapiens
4 PCGF5  
Affinity Capture-MS Homo sapiens
5 UBE2A  
Co-fractionation Homo sapiens
6 PAXIP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
7 UTP11L  
Affinity Capture-MS Homo sapiens
8 ZNF566  
Two-hybrid Homo sapiens
9 CEP170 9859
Affinity Capture-MS Homo sapiens
10 RBMXL1  
Affinity Capture-MS Homo sapiens
11 MAP4 4134
Affinity Capture-MS Homo sapiens
12 SEMG2 6407
Affinity Capture-MS Homo sapiens
13 VHL  
Two-hybrid Homo sapiens
14 MRPL52  
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 WDR62  
Affinity Capture-MS Homo sapiens
17 MRFAP1L1  
Affinity Capture-MS Homo sapiens
18 PRPF38B 55119
Affinity Capture-MS Homo sapiens
19 ADAM9 8754
Affinity Capture-MS Homo sapiens
20 RRP12 23223
Affinity Capture-MS Homo sapiens
21 CLIP1 6249
Affinity Capture-MS Homo sapiens
22 KLHL40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RCBTB2  
Affinity Capture-MS Homo sapiens
24 MBD1  
Affinity Capture-MS Homo sapiens
25 MTUS2 23281
Affinity Capture-Western Homo sapiens
26 FBXO11  
Affinity Capture-MS Homo sapiens
27 BRD4 23476
Affinity Capture-MS Homo sapiens
28 CCDC47 57003
Cross-Linking-MS (XL-MS) Homo sapiens
29 FBXO5  
Affinity Capture-MS Homo sapiens
30 ESPL1  
Affinity Capture-MS Homo sapiens
31 CDCA8 55143
Affinity Capture-MS Homo sapiens
32 POP1 10940
Affinity Capture-MS Homo sapiens
33 STK4 6789
Affinity Capture-MS Homo sapiens
34 WDR5 11091
Co-fractionation Homo sapiens
35 CSNK2B 1460
Two-hybrid Homo sapiens
36 TNFRSF1B  
Affinity Capture-MS Homo sapiens
37 SDHA 6389
Affinity Capture-MS Homo sapiens
38 RBPJ 3516
Affinity Capture-MS Homo sapiens
39 KIF14 9928
Affinity Capture-MS Homo sapiens
40 SMC4 10051
Co-fractionation Homo sapiens
41 C15orf59  
Affinity Capture-MS Homo sapiens
42 AURKA 6790
Biochemical Activity Homo sapiens
43 BRPF1  
Affinity Capture-MS Homo sapiens
44 MYO18A 399687
Affinity Capture-MS Homo sapiens
45 SRGAP3  
Affinity Capture-MS Homo sapiens
46 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CENPH  
Reconstituted Complex Homo sapiens
48 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
49 RPL9 6133
Affinity Capture-MS Homo sapiens
50 PARK2  
Affinity Capture-MS Homo sapiens
51 MAPRE1 22919
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 RELL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ATP6V0C 527
Affinity Capture-MS Homo sapiens
54 RPL5 6125
Affinity Capture-MS Homo sapiens
55 ACTR3B 57180
Affinity Capture-MS Homo sapiens
56 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 SAR1B 51128
Affinity Capture-MS Homo sapiens
59 RRP36 88745
Affinity Capture-MS Homo sapiens
60 PLK1 5347
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
61 DCTN1 1639
Proximity Label-MS Homo sapiens
62 RBMX2  
Affinity Capture-MS Homo sapiens
63 DHFRL1  
Proximity Label-MS Homo sapiens
64 ANAPC4 29945
Affinity Capture-MS Homo sapiens
65 HECTD1 25831
Affinity Capture-MS Homo sapiens
66 PSMA5 5686
Affinity Capture-MS Homo sapiens
67 SDF4 51150
Affinity Capture-MS Homo sapiens
68 MYL12A 10627
Affinity Capture-MS Homo sapiens
69 KRT79 338785
Affinity Capture-MS Homo sapiens
70 CEBPA  
Protein-peptide Homo sapiens
71 SGMS2 166929
Affinity Capture-MS Homo sapiens
72 KIF2C 11004
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
73 RMDN3 55177
Proximity Label-MS Homo sapiens
74 PAPLN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PCBP2 5094
Co-fractionation Homo sapiens
76 CALML3 810
Affinity Capture-MS Homo sapiens
77 HABP4  
Affinity Capture-MS Homo sapiens
78 TJP1 7082
Affinity Capture-MS Homo sapiens
79 STK3 6788
Affinity Capture-MS Homo sapiens
80 SGOL1  
Affinity Capture-MS Homo sapiens
81 EGLN3  
Affinity Capture-MS Homo sapiens
82 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 CUL3 8452
Affinity Capture-MS Homo sapiens
84 HSPA14 51182
Co-fractionation Homo sapiens
85 TYW3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SNRNP27  
Affinity Capture-MS Homo sapiens
87 ELAC2 60528
Co-fractionation Homo sapiens
88 NDEL1 81565
Two-hybrid Homo sapiens
89 RPL7 6129
Affinity Capture-MS Homo sapiens
90 HIST3H3 8290
Protein-peptide Homo sapiens
91 FN1 2335
Affinity Capture-MS Homo sapiens
92 NXF1 10482
Affinity Capture-RNA Homo sapiens
93 AURKB 9212
Affinity Capture-Western Homo sapiens
94 SEMG1 6406
Affinity Capture-MS Homo sapiens
95 MAPRE3  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 BIRC6 57448
Affinity Capture-MS Homo sapiens
97 RBM26 64062
Affinity Capture-MS Homo sapiens
98 RPA3 6119
Proximity Label-MS Homo sapiens
99 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
100 ANKS3  
Affinity Capture-MS Homo sapiens
101 SLC6A15 55117
Affinity Capture-MS Homo sapiens
102 SKIV2L2 23517
Co-fractionation Homo sapiens
103 CCDC101  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 HAX1  
Proximity Label-MS Homo sapiens
105 BMP1 649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 NFATC2  
Affinity Capture-MS Homo sapiens
107 KIF2B 84643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 KIF2A 3796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
110 C10orf88  
Affinity Capture-MS Homo sapiens
111 PRRC2A 7916
Affinity Capture-MS Homo sapiens
112 RPS25 6230
Affinity Capture-MS Homo sapiens
113 HORMAD2  
Affinity Capture-MS Homo sapiens
114 TBC1D7  
Affinity Capture-MS Homo sapiens
115 DDHD1 80821
Affinity Capture-MS Homo sapiens
116 DUSP3 1845
Affinity Capture-MS Homo sapiens
117 LSM14A 26065
Affinity Capture-MS Homo sapiens
118 MAGEA9  
Affinity Capture-MS Homo sapiens
119 WDR18 57418
Affinity Capture-MS Homo sapiens
120 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 CLASP1 23332
Affinity Capture-MS Homo sapiens
122 MOV10 4343
Affinity Capture-RNA Homo sapiens
123 KRT38 8687
Affinity Capture-MS Homo sapiens
124 KIF18B 146909
Affinity Capture-MS Homo sapiens
125 ARRB1 408
Affinity Capture-MS Homo sapiens
126 DNM3 26052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 GOT1 2805
Co-fractionation Homo sapiens
128 Rrbp1  
Affinity Capture-MS Mus musculus
129 KXD1 79036
Affinity Capture-MS Homo sapiens
130 BCCIP 56647
Affinity Capture-MS Homo sapiens
131 SNW1 22938
Affinity Capture-MS Homo sapiens
132 Kif2a 16563
Affinity Capture-MS Mus musculus
133 TEX10 54881
Affinity Capture-MS Homo sapiens
134 PRMT1 3276
Affinity Capture-MS Homo sapiens
135 CDC5L 988
Affinity Capture-MS Homo sapiens
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