Gene description for CLASP1
Gene name cytoplasmic linker associated protein 1
Gene symbol CLASP1
Other names/aliases MAST1
Species Homo sapiens
 Database cross references - CLASP1
ExoCarta ExoCarta_23332
Vesiclepedia VP_23332
Entrez Gene 23332
HGNC 17088
MIM 605852
UniProt Q7Z460  
 CLASP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Malignant pleural effusions 14975938    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CLASP1
Molecular Function
    dystroglycan binding GO:0002162 IPI
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    microtubule binding GO:0008017 IMP
    kinetochore binding GO:0043515 IBA
    kinetochore binding GO:0043515 IMP
    microtubule plus-end binding GO:0051010 IDA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IGI
    microtubule cytoskeleton organization GO:0000226 IMP
    microtubule bundle formation GO:0001578 IMP
    vesicle targeting GO:0006903 IMP
    microtubule nucleation GO:0007020 IMP
    negative regulation of microtubule depolymerization GO:0007026 IGI
    negative regulation of microtubule depolymerization GO:0007026 IMP
    Golgi organization GO:0007030 IMP
    mitotic spindle organization GO:0007052 IMP
    establishment or maintenance of cell polarity GO:0007163 NAS
    exit from mitosis GO:0010458 IMP
    regulation of gastrulation GO:0010470 IMP
    positive regulation of epithelial cell migration GO:0010634 IMP
    regulation of epithelial to mesenchymal transition GO:0010717 IMP
    astral microtubule organization GO:0030953 IMP
    microtubule organizing center organization GO:0031023 IMP
    negative regulation of microtubule polymerization or depolymerization GO:0031111 IMP
    positive regulation of microtubule polymerization GO:0031116 ISS
    microtubule anchoring GO:0034453 IMP
    establishment of mitotic spindle localization GO:0040001 IBA
    establishment of mitotic spindle localization GO:0040001 IMP
    positive regulation of exocytosis GO:0045921 IMP
    establishment of spindle orientation GO:0051294 IDA
    establishment of spindle orientation GO:0051294 IGI
    establishment of spindle orientation GO:0051294 IMP
    cell division GO:0051301 IDA
    negative regulation of stress fiber assembly GO:0051497 IMP
    regulation of focal adhesion assembly GO:0051893 IMP
    regulation of microtubule cytoskeleton organization GO:0070507 IGI
    positive regulation of extracellular matrix disassembly GO:0090091 IMP
    mitotic spindle assembly GO:0090307 IBA
    mitotic spindle assembly GO:0090307 ISS
    negative regulation of wound healing, spreading of epidermal cells GO:1903690 IMP
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IGI
    positive regulation of basement membrane assembly involved in embryonic body morphogenesis GO:1904261 IMP
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IDA
    Golgi apparatus GO:0005794 IDA
    centrosome GO:0005813 IDA
    microtubule organizing center GO:0005815 IBA
    kinetochore microtubule GO:0005828 TAS
    cytosol GO:0005829 TAS
    spindle microtubule GO:0005876 IBA
    spindle microtubule GO:0005876 IDA
    cytoplasmic microtubule GO:0005881 IBA
    cytoplasmic microtubule GO:0005881 IDA
    focal adhesion GO:0005925 IDA
    cell cortex GO:0005938 IDA
    membrane GO:0016020 HDA
    cortical microtubule cytoskeleton GO:0030981 IDA
    centrosomal corona GO:0031592 IDA
    microtubule plus-end GO:0035371 ISS
    basal cortex GO:0045180 IBA
    basal cortex GO:0045180 IDA
    mitotic spindle GO:0072686 IBA
 Experiment description of studies that identified CLASP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 51
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CLASP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAPPC2 6399
Affinity Capture-MS Homo sapiens
2 MIS12  
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 CLIP1 6249
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CENPE 1062
Affinity Capture-MS Homo sapiens
6 ACTR2 10097
Affinity Capture-MS Homo sapiens
7 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
8 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MAP4 4134
Two-hybrid Homo sapiens
10 Soga1  
Affinity Capture-MS Mus musculus
11 CAMSAP2  
Proximity Label-MS Homo sapiens
12 CBY1  
Affinity Capture-MS Homo sapiens
13 ARHGEF38  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 FAXC  
Affinity Capture-MS Homo sapiens
15 EIF4ENIF1 56478
Two-hybrid Homo sapiens
16 ARL6IP6  
Affinity Capture-MS Homo sapiens
17 MARK2 2011
Affinity Capture-MS Homo sapiens
18 JAKMIP1 152789
Affinity Capture-MS Homo sapiens
19 DCTN4 51164
Affinity Capture-MS Homo sapiens
20 Cep55 74107
Affinity Capture-MS Mus musculus
21 FBXW7  
Affinity Capture-MS Homo sapiens
22 ACTR1A 10121
Affinity Capture-MS Homo sapiens
23 RCBTB2  
Affinity Capture-MS Homo sapiens
24 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 Cenpe  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
26 CKAP5 9793
Two-hybrid Homo sapiens
27 KIF2C 11004
Affinity Capture-MS Homo sapiens
28 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MECP2 4204
Affinity Capture-MS Homo sapiens
30 DYNLT1 6993
Affinity Capture-MS Homo sapiens
31 CCDC102A 92922
Affinity Capture-MS Homo sapiens
32 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MTNR1B  
Two-hybrid Homo sapiens
34 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KRT8 3856
Proximity Label-MS Homo sapiens
36 RNF41  
Affinity Capture-MS Homo sapiens
37 MAPRE1 22919
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 GCC2 9648
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
39 RPL18A 6142
Cross-Linking-MS (XL-MS) Homo sapiens
40 TUBA1B 10376
Affinity Capture-MS Homo sapiens
41 VCP 7415
Affinity Capture-MS Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 DCTN1 1639
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 HECTD1 25831
Affinity Capture-MS Homo sapiens
46 CEBPA  
Protein-peptide Homo sapiens
47 SYBU  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 HYPM  
Affinity Capture-MS Homo sapiens
49 GAN 8139
Affinity Capture-MS Homo sapiens
50 DCTN3 11258
Affinity Capture-MS Homo sapiens
51 XPO1 7514
Affinity Capture-MS Homo sapiens
52 LAMP1 3916
Affinity Capture-MS Homo sapiens
53 Rbm14  
Two-hybrid Mus musculus
54 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 TUBB4B 10383
Affinity Capture-MS Homo sapiens
56 MTNR1A  
Two-hybrid Homo sapiens
57 Clasp2  
Affinity Capture-MS Mus musculus
58 PHKG2 5261
Affinity Capture-MS Homo sapiens
59 MYO1C 4641
Affinity Capture-MS Homo sapiens
60 MAPRE3  
Proximity Label-MS Homo sapiens
61 ZW10 9183
Co-fractionation Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 NIN 51199
Affinity Capture-MS Homo sapiens
64 Rpl35 66489
Affinity Capture-MS Mus musculus
65 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
66 GYPA  
Affinity Capture-MS Homo sapiens
67 KIF2A 3796
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
69 MACF1 23499
Affinity Capture-MS Homo sapiens
70 CLASP2 23122
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
71 CENPJ 55835
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ARL8A 127829
Affinity Capture-MS Homo sapiens
73 CAPZA2 830
Affinity Capture-MS Homo sapiens
74 DCTN2 10540
Affinity Capture-MS Homo sapiens
75 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
76 DDX58 23586
Affinity Capture-RNA Homo sapiens
77 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
78 MED18  
Affinity Capture-MS Homo sapiens
79 Mapre1 13589
Affinity Capture-MS Mus musculus
80 JAKMIP2  
Two-hybrid Homo sapiens
81 DYNLRB1 83658
Affinity Capture-MS Homo sapiens
82 Rrbp1  
Affinity Capture-MS Mus musculus
83 CLIP2 7461
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
84 CLIP4  
Affinity Capture-MS Homo sapiens
85 DYNC1LI1 51143
Affinity Capture-MS Homo sapiens
86 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CLASP1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
Anchoring of the basal body to the plasma membrane TAS Reactome
AURKA Activation by TPX2 TAS Reactome
Axon guidance IEA Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Centrosome maturation TAS Reactome
Cilium Assembly TAS Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
G2/M Transition TAS Reactome
Loss of Nlp from mitotic centrosomes TAS Reactome
Loss of proteins required for interphase microtubule organization from the centrosome TAS Reactome
M Phase TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Nervous system development IEA Reactome
Organelle biogenesis and maintenance TAS Reactome
Recruitment of mitotic centrosome proteins and complexes TAS Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Regulation of PLK1 Activity at G2/M Transition TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
Role of ABL in ROBO-SLIT signaling IEA Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by ROBO receptors IEA Reactome





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