Gene description for CLASP1
Gene name cytoplasmic linker associated protein 1
Gene symbol CLASP1
Other names/aliases MAST1
Species Homo sapiens
 Database cross references - CLASP1
ExoCarta ExoCarta_23332
Entrez Gene 23332
HGNC 17088
MIM 605852
UniProt Q7Z460  
 CLASP1 identified in exosomes derived from the following tissue/cell type
Malignant pleural effusions 14975938    
 Gene ontology annotations for CLASP1
Molecular Function
    microtubule plus-end binding GO:0051010 IDA
    kinetochore binding GO:0043515 IMP
    microtubule binding GO:0008017 IMP
    protein binding GO:0005515 IPI
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    establishment of spindle orientation GO:0051294 IMP
    microtubule cytoskeleton organization GO:0000226 IGI
    negative regulation of microtubule depolymerization GO:0007026 IMP
    cell division GO:0051301 IDA
    axon guidance GO:0007411 TAS
    microtubule bundle formation GO:0001578 IMP
    microtubule anchoring GO:0034453 IMP
    organelle organization GO:0006996 TAS
    exit from mitosis GO:0010458 IMP
    mitotic cell cycle GO:0000278 TAS
    microtubule organizing center organization GO:0031023 IMP
    establishment or maintenance of cell polarity GO:0007163 NAS
    microtubule nucleation GO:0007020 IMP
    negative regulation of microtubule polymerization or depolymerization GO:0031111 IMP
Subcellular Localization
    cytoplasmic microtubule GO:0005881 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    kinetochore GO:0000776 IDA
    spindle microtubule GO:0005876 IDA
    membrane GO:0016020 IDA
    centrosome GO:0005813 IDA
    centrosomal corona GO:0031592 IDA
    extracellular exosome GO:0070062 IDA
    condensed chromosome kinetochore GO:0000777 IEA
    kinetochore microtubule GO:0005828 TAS
    cell cortex GO:0005938 IDA
    cortical microtubule cytoskeleton GO:0030981 IDA
 Experiment description of studies that identified CLASP1 in exosomes
1
Experiment ID 51
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for CLASP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLIP2 7461
Reconstituted Complex Homo sapiens
2 YWHAG 7532
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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