Gene description for MACF1
Gene name microtubule-actin crosslinking factor 1
Gene symbol MACF1
Other names/aliases ABP620
ACF7
MACF
OFC4
Species Homo sapiens
 Database cross references - MACF1
ExoCarta ExoCarta_23499
Entrez Gene 23499
HGNC 13664
MIM 608271
UniProt Q9UPN3  
 MACF1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for MACF1
Molecular Function
    actin binding GO:0003779 IDA
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 NAS
    poly(A) RNA binding GO:0044822 IDA
    ATPase activity GO:0016887 ISS
    calcium ion binding GO:0005509 IEA
    microtubule binding GO:0008017 IEA
Biological Process
    positive regulation of axon extension GO:0045773 IDA
    wound healing GO:0042060 ISS
    metabolic process GO:0008152 ISS
    Golgi to plasma membrane protein transport GO:0043001 IDA
    Wnt signaling pathway GO:0016055 IEA
    positive regulation of Wnt signaling pathway GO:0030177 ISS
    regulation of focal adhesion assembly GO:0051893 ISS
    regulation of microtubule-based process GO:0032886 IMP
    establishment or maintenance of cell polarity GO:0007163 IEA
    mesoderm formation GO:0001707 IEA
    regulation of epithelial cell migration GO:0010632 ISS
    posttranslational protein targeting to membrane GO:0006620 IEA
    cell cycle arrest GO:0007050 IEA
Subcellular Localization
    microtubule GO:0005874 IDA
    cytoskeleton GO:0005856 NAS
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 ISS
    ruffle membrane GO:0032587 IDA
    Golgi apparatus GO:0005794 IDA
    actin cytoskeleton GO:0015629 IEA
 Experiment description of studies that identified MACF1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MACF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MACF1 is involved
No pathways found





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