Gene description for NASP
Gene name nuclear autoantigenic sperm protein (histone-binding)
Gene symbol NASP
Other names/aliases FLB7527
PRO1999
Species Homo sapiens
 Database cross references - NASP
ExoCarta ExoCarta_4678
Entrez Gene 4678
HGNC 7644
MIM 603185
UniProt P49321  
 NASP identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NASP
Molecular Function
    histone binding GO:0042393 IEA
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 IDA
    Hsp90 protein binding GO:0051879 ISS
Biological Process
    nucleosome assembly GO:0006334 IDA
    protein transport GO:0015031 IEA
    DNA replication-independent nucleosome assembly GO:0006336 IDA
    cell proliferation GO:0008283 ISS
    DNA replication GO:0006260 ISS
    DNA replication-dependent nucleosome assembly GO:0006335 IDA
    blastocyst development GO:0001824 ISS
    histone exchange GO:0043486 ISS
    cell cycle GO:0007049 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    nucleoplasm GO:0005654 IDA
    nuclear chromatin GO:0000790 IDA
    nucleus GO:0005634 ISS
    protein complex GO:0043234 IDA
 Experiment description of studies that identified NASP in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NASP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST1H1A 3024
Invivo Homo sapiens
Invitro Homo sapiens
2 ULK2  
Two-hybrid Homo sapiens
3 TRAF6 7189
Affinity Capture-MS Homo sapiens
4 HIST1H3H 8357
Two-hybrid Homo sapiens
5 LUC7L2  
Two-hybrid Homo sapiens
6 HIST2H3C 126961
Two-hybrid Homo sapiens
7 HIST1H3G 8355
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NASP is involved
No pathways found





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