Gene description for HIST1H4L
Gene name histone cluster 1, H4l
Gene symbol HIST1H4L
Other names/aliases H4.k
H4/k
H4FK
Species Homo sapiens
 Database cross references - HIST1H4L
ExoCarta ExoCarta_8368
Vesiclepedia VP_8368
Entrez Gene 8368
HGNC 4791
MIM 602831
UniProt P62805  
 HIST1H4L identified in sEVs derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Endothelial cells 26027894    
Keratinocytes 19530224    
Keratinocytes 19530224    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for HIST1H4L
Molecular Function
    DNA binding GO:0003677 IBA
    DNA binding GO:0003677 TAS
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    structural constituent of chromatin GO:0030527 IDA
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    chromatin organization GO:0006325 NAS
    nucleosome assembly GO:0006334 IBA
    nucleosome assembly GO:0006334 IDA
    telomere organization GO:0032200 TAS
    negative regulation of megakaryocyte differentiation GO:0045653 IDA
    protein localization to CENP-A containing chromatin GO:0061644 NAS
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    nucleosome GO:0000786 IDA
    nucleosome GO:0000786 IPI
    nucleosome GO:0000786 TAS
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    CENP-A containing nucleosome GO:0043505 IPI
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HIST1H4L in sEVs
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
11
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
14
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
18
Experiment ID 139
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
19
Experiment ID 140
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
20
Experiment ID 141
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
21
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
22
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
23
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
24
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
25
Experiment ID 146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
26
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
27
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HIST1H4L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 CHAF1A  
Affinity Capture-MS Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
4 TONSL  
Affinity Capture-MS Homo sapiens
5 TMPO 7112
Affinity Capture-MS Homo sapiens
6 ASF1A 25842
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 HDGF 3068
Affinity Capture-MS Homo sapiens
8 OBSL1 23363
Affinity Capture-MS Homo sapiens
9 HAT1 8520
Affinity Capture-MS Homo sapiens
10 RIN3  
Affinity Capture-MS Homo sapiens
11 ASF1B  
Affinity Capture-MS Homo sapiens
12 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
13 H2AFZ 3015
Affinity Capture-Western Homo sapiens
14 RLIM 51132
Affinity Capture-MS Homo sapiens
15 CHAF1B  
Affinity Capture-MS Homo sapiens
16 CUL5 8065
Affinity Capture-MS Homo sapiens
17 H2AFX 3014
Affinity Capture-MS Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 NPAT  
Affinity Capture-MS Homo sapiens
20 CAND1 55832
Affinity Capture-MS Homo sapiens
21 MCM4 4173
Affinity Capture-MS Homo sapiens
22 H3F3B 3021
Affinity Capture-MS Homo sapiens
23 RBBP4 5928
Affinity Capture-MS Homo sapiens
24 FN1 2335
Affinity Capture-MS Homo sapiens
25 PARP2  
Affinity Capture-MS Homo sapiens
26 ATRX 546
Affinity Capture-MS Homo sapiens
27 TRIM24  
Co-crystal Structure Homo sapiens
28 PPTC7 160760
Affinity Capture-MS Homo sapiens
29 SHPRH  
Affinity Capture-MS Homo sapiens
30 SSRP1 6749
Affinity Capture-MS Homo sapiens
31 IPO4 79711
Affinity Capture-MS Homo sapiens
32 MCM2 4171
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CBX5 23468
Affinity Capture-MS Homo sapiens
34 HJURP  
Affinity Capture-MS Homo sapiens
35 COPS5 10987
Affinity Capture-MS Homo sapiens
36 MMS22L  
Affinity Capture-MS Homo sapiens
37 HIRA  
Affinity Capture-MS Homo sapiens
38 CUL7 9820
Affinity Capture-MS Homo sapiens
39 RBBP7 5931
Affinity Capture-MS Homo sapiens
40 ZNF512B  
Affinity Capture-MS Homo sapiens
41 CUL2 8453
Affinity Capture-MS Homo sapiens
42 BRD4 23476
Affinity Capture-MS Homo sapiens
43 MCM6 4175
Affinity Capture-MS Homo sapiens
44 BRD3 8019
Affinity Capture-MS Homo sapiens
45 SUPT16H 11198
Affinity Capture-MS Homo sapiens
46 RAD21 5885
Affinity Capture-Western Homo sapiens
47 DAXX  
Affinity Capture-MS Homo sapiens
48 CCDC8  
Affinity Capture-MS Homo sapiens
49 ITGA4 3676
Affinity Capture-MS Homo sapiens
50 RBBP8  
Affinity Capture-MS Homo sapiens
51 UCHL5 51377
Reconstituted Complex Homo sapiens
52 NASP 4678
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HIST1H4L is involved
PathwayEvidenceSource
Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 TAS Reactome
Activation of anterior HOX genes in hindbrain development during early embryogenesis IEA Reactome
Activation of HOX genes during differentiation IEA Reactome
Amyloid fiber formation TAS Reactome
Assembly of the ORC complex at the origin of replication TAS Reactome
Assembly of the pre-replicative complex TAS Reactome
B-WICH complex positively regulates rRNA expression TAS Reactome
Base Excision Repair TAS Reactome
Base Excision Repair IEA Reactome
Base-Excision Repair, AP Site Formation TAS Reactome
Base-Excision Repair, AP Site Formation IEA Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Chromatin modifications during the maternal to zygotic transition (MZT) IEA Reactome
Chromatin modifying enzymes TAS Reactome
Chromatin modifying enzymes IEA Reactome
Chromatin organization TAS Reactome
Chromatin organization IEA Reactome
Chromosome Maintenance TAS Reactome
Cleavage of the damaged purine TAS Reactome
Cleavage of the damaged purine IEA Reactome
Cleavage of the damaged pyrimidine IEA Reactome
Condensation of Prophase Chromosomes TAS Reactome
Defective pyroptosis TAS Reactome
Deposition of new CENPA-containing nucleosomes at the centromere TAS Reactome
Depurination TAS Reactome
Depurination IEA Reactome
Depyrimidination IEA Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Diseases of programmed cell death TAS Reactome
DNA Damage/Telomere Stress Induced Senescence TAS Reactome
DNA Double Strand Break Response TAS Reactome
DNA Double-Strand Break Repair TAS Reactome
DNA methylation IEA Reactome
DNA Repair TAS Reactome
DNA Repair IEA Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes IEA Reactome
Epigenetic regulation by WDR5-containing histone modifying complexes TAS Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of adipogenesis genes by MLL3 and MLL4 complexes TAS Reactome
Epigenetic regulation of gene expression TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes IEA Reactome
Epigenetic regulation of gene expression by MLL3 and MLL4 complexes TAS Reactome
ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression IEA Reactome
ESR-mediated signaling TAS Reactome
Estrogen-dependent gene expression TAS Reactome
Formation of the beta-catenin:TCF transactivating complex TAS Reactome
Formation of the beta-catenin:TCF transactivating complex IEA Reactome
G2/M Checkpoints TAS Reactome
G2/M DNA damage checkpoint TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA TAS Reactome
Generic Transcription Pathway TAS Reactome
Generic Transcription Pathway IEA Reactome
HATs acetylate histones TAS Reactome
HATs acetylate histones IEA Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HDACs deacetylate histones TAS Reactome
HDMs demethylate histones TAS Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) TAS Reactome
Homology Directed Repair TAS Reactome
Infectious disease TAS Reactome
Inhibition of DNA recombination at telomere TAS Reactome
M Phase TAS Reactome
Maternal to zygotic transition (MZT) IEA Reactome
Meiosis IEA Reactome
Meiotic recombination IEA Reactome
Meiotic synapsis IEA Reactome
Metabolism of proteins TAS Reactome
Mitotic Prophase TAS Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis IEA Reactome
MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis TAS Reactome
Negative epigenetic regulation of rRNA expression TAS Reactome
Negative epigenetic regulation of rRNA expression IEA Reactome
Nonhomologous End-Joining (NHEJ) TAS Reactome
NoRC negatively regulates rRNA expression TAS Reactome
NoRC negatively regulates rRNA expression IEA Reactome
Nucleosome assembly TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Packaging Of Telomere Ends TAS Reactome
PKMTs methylate histone lysines TAS Reactome
Positive epigenetic regulation of rRNA expression TAS Reactome
Positive epigenetic regulation of rRNA expression IEA Reactome
Post-translational protein modification TAS Reactome
PRC2 methylates histones and DNA TAS Reactome
Pre-NOTCH Expression and Processing IEA Reactome
Pre-NOTCH Transcription and Translation IEA Reactome
Processing of DNA double-strand break ends TAS Reactome
Recognition and association of DNA glycosylase with site containing an affected purine TAS Reactome
Recognition and association of DNA glycosylase with site containing an affected purine IEA Reactome
Recognition and association of DNA glycosylase with site containing an affected pyrimidine IEA Reactome
Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks TAS Reactome
Regulation of endogenous retroelements TAS Reactome
Regulation of endogenous retroelements IEA Reactome
Regulation of endogenous retroelements by KRAB-ZFP proteins TAS Reactome
Regulation of endogenous retroelements by KRAB-ZFP proteins IEA Reactome
Regulation of endogenous retroelements by Piwi-interacting RNAs (piRNAs) IEA Reactome
Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex TAS Reactome
Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex IEA Reactome
Replacement of protamines by nucleosomes in the male pronucleus IEA Reactome
Reproduction IEA Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases activate PKNs TAS Reactome
RMTs methylate histone arginines TAS Reactome
RNA Polymerase I Promoter Clearance TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Promoter Opening TAS Reactome
RNA Polymerase I Transcription TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function TAS Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
SIRT1 negatively regulates rRNA expression TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMOylation TAS Reactome
SUMOylation of chromatin organization proteins TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCF dependent signaling in response to WNT IEA Reactome
Telomere Maintenance TAS Reactome
Transcriptional regulation by RUNX1 TAS Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional regulation of granulopoiesis IEA Reactome
Viral Infection Pathways TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here