Gene description for RLIM
Gene name ring finger protein, LIM domain interacting
Gene symbol RLIM
Other names/aliases NY-REN-43
RNF12
Species Homo sapiens
 Database cross references - RLIM
ExoCarta ExoCarta_51132
Entrez Gene 51132
HGNC 13429
MIM 300379
UniProt Q9NVW2  
 RLIM identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for RLIM
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISS
    ligase activity GO:0016874 IEA
    transcription corepressor activity GO:0003714 NAS
    zinc ion binding GO:0008270 IEA
Biological Process
    random inactivation of X chromosome GO:0060816 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 ISS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IEA
    protein ubiquitination GO:0016567 ISS
    negative regulation of transcription, DNA-templated GO:0045892 NAS
    transcription, DNA-templated GO:0006351 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 ISS
    transcriptional repressor complex GO:0017053 NAS
 Experiment description of studies that identified RLIM in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for RLIM
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RLIM is involved
No pathways found





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