Experiment description of studies that identified PCBP3 in exosomes

1 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for PCBP3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Igf2bp2 319765 Two-hybrid Mus musculus 2 REC8   Affinity Capture-MS Homo sapiens 3 AKR1B10 57016 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 GNPDA2 132789 Affinity Capture-MS Homo sapiens 5 ZNF514   Affinity Capture-MS Homo sapiens 6 Celf1   Two-hybrid Mus musculus 7 AGXT   Affinity Capture-MS Homo sapiens 8 MSRB2   Affinity Capture-MS Homo sapiens 9 MYEOV   Affinity Capture-MS Homo sapiens 10 PTX3 5806 Affinity Capture-MS Homo sapiens 11 COPRS   Affinity Capture-MS Homo sapiens 12 QKI 9444 Two-hybrid Homo sapiens 13 GAPDHS 26330 Affinity Capture-MS Homo sapiens 14 Aif1l   Affinity Capture-MS Mus musculus 15 KIF20A 10112 Affinity Capture-MS Homo sapiens 16 GCLM 2730 Affinity Capture-MS Homo sapiens 17 DVL3 1857 Affinity Capture-MS Homo sapiens 18 GBP2   Affinity Capture-MS Homo sapiens 19 SLC39A3   Affinity Capture-MS Homo sapiens 20 RNH1 6050 Affinity Capture-MS Homo sapiens 21 PLOD3 8985 Affinity Capture-MS Homo sapiens 22 TSTA3 7264 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 PFKL 5211 Co-fractionation Homo sapiens 24 METTL25   Affinity Capture-MS Homo sapiens 25 KIF23 9493 Affinity Capture-MS Homo sapiens 26 MATR3 9782 Two-hybrid Homo sapiens 27 PPP1R18 170954 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 PRC1 9055 Affinity Capture-MS Homo sapiens 29 BBOX1 8424 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 THTPA   Affinity Capture-MS Homo sapiens 31 PIF1   Affinity Capture-MS Homo sapiens 32 FBLN2 2199 Affinity Capture-MS Homo sapiens 33 MCTS1 28985 Affinity Capture-MS Homo sapiens 34 CCDC6 8030 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 EIF3E 3646 Affinity Capture-MS Homo sapiens 36 FAM127C   Affinity Capture-MS Homo sapiens 37 COLGALT2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 RAB6B 51560 Affinity Capture-MS Homo sapiens 39 MYCN   Affinity Capture-MS Homo sapiens 40 SERBP1 26135 Affinity Capture-MS Homo sapiens 41 Igf2bp3 140488 Two-hybrid Mus musculus 42 SREK1 140890 Two-hybrid Homo sapiens 43 KIF14 9928 Affinity Capture-MS Homo sapiens 44 PARPBP   Affinity Capture-MS Homo sapiens 45 DLD 1738 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 ARL2BP 23568 Affinity Capture-MS Homo sapiens 47 RBM47 54502 Two-hybrid Homo sapiens 48 SNRPA 6626 Two-hybrid Homo sapiens 49 SRSF3 6428 Two-hybrid Homo sapiens 50 FAM96B 51647 Affinity Capture-MS Homo sapiens 51 APOBEC3C 27350 Two-hybrid Homo sapiens 52 HDAC10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 53 CELF4   Two-hybrid Homo sapiens 54 MYDGF 56005 Affinity Capture-MS Homo sapiens 55 SDF2L1 23753 Affinity Capture-MS Homo sapiens 56 CHMP4B 128866 Affinity Capture-MS Homo sapiens 57 EIF4EBP1   Affinity Capture-MS Homo sapiens 58 PRKCE 5581 Affinity Capture-MS Homo sapiens 59 PCBP2 5094 Two-hybrid Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Affinity Capture-MS Homo sapiens 60 ANLN 54443 Affinity Capture-MS Homo sapiens 61 ZBTB2 57621 Affinity Capture-MS Homo sapiens 62 RBM39 9584 Affinity Capture-MS Homo sapiens 63 CDC73   Affinity Capture-MS Homo sapiens 64 ECD   Affinity Capture-MS Homo sapiens 65 Rbm47   Two-hybrid Mus musculus 66 ECT2 1894 Affinity Capture-MS Homo sapiens 67 OCRL 4952 Affinity Capture-MS Homo sapiens 68 RLIM 51132 Affinity Capture-MS Homo sapiens 69 Rbm14   Two-hybrid Mus musculus 70 SGOL1   Affinity Capture-MS Homo sapiens 71 TYW3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 72 B4GALNT3   Affinity Capture-MS Homo sapiens 73 PSMA2 5683 Affinity Capture-MS Homo sapiens 74 KLRD1   Affinity Capture-MS Homo sapiens 75 NUP43 348995 Affinity Capture-MS Homo sapiens 76 FTH1 2495 Affinity Capture-Western Homo sapiens 77 Pcbp1 23983 Two-hybrid Mus musculus 78 KRT81   Two-hybrid Homo sapiens 79 PCBP1 5093 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 80 SPEN 23013 Co-fractionation Homo sapiens 81 BAG1 573 Affinity Capture-MS Homo sapiens 82 Ube2i   Two-hybrid Mus musculus 83 ENO2 2026 Affinity Capture-MS Homo sapiens 84 DDRGK1 65992 Affinity Capture-MS Homo sapiens 85 Eif2s1 13665 Two-hybrid Mus musculus 86 ZC3HAV1 56829 Two-hybrid Homo sapiens 87 Sf3b2   Two-hybrid Mus musculus 88 PDSS1   Affinity Capture-MS Homo sapiens 89 Cirbp   Two-hybrid Mus musculus 90 LGALSL 29094 Affinity Capture-MS Homo sapiens 91 Eif3c 56347 Two-hybrid Mus musculus 92 FCGR3B   Affinity Capture-MS Homo sapiens 93 CA6 765 Affinity Capture-MS Homo sapiens 94 Elavl4   Two-hybrid Mus musculus 95 FOLR1 2348 Affinity Capture-MS Homo sapiens 96 PDCD2L 84306 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 97 RUVBL2 10856 Affinity Capture-MS Homo sapiens 98 HECTD3 79654 Affinity Capture-MS Homo sapiens 99 COMMD5 28991 Affinity Capture-MS Homo sapiens 100 ACAA2 10449 Co-fractionation Homo sapiens 101 TLE1 7088 Affinity Capture-MS Homo sapiens 102 OR4X2   Affinity Capture-MS Homo sapiens 103 PTBP1 5725 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 104 B4GAT1 11041 Affinity Capture-MS Homo sapiens 105 FBXO4 26272 Affinity Capture-MS Homo sapiens 106 PUF60 22827 Two-hybrid Homo sapiens 107 PTBP2   Two-hybrid Homo sapiens 108 DDIT4L   Two-hybrid Homo sapiens 109 UFL1 23376 Affinity Capture-MS Homo sapiens 110 RUVBL1 8607 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 111 PSMA5 5686 Affinity Capture-MS Homo sapiens 112 HNRNPLL 92906 Two-hybrid Homo sapiens 113 TRMT5   Affinity Capture-MS Homo sapiens 114 KCTD17 79734 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 115 RHOD 29984 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 116 MYC   Affinity Capture-MS Homo sapiens 117 Srsf3 20383 Two-hybrid Mus musculus 118 PLD6   Affinity Capture-MS Homo sapiens 119 DCP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 120 WNT16   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which PCBP3 is involved

No pathways found