Gene description for TLE1
Gene name transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Gene symbol TLE1
Other names/aliases ESG
ESG1
GRG1
Species Homo sapiens
 Database cross references - TLE1
ExoCarta ExoCarta_7088
Vesiclepedia VP_7088
Entrez Gene 7088
HGNC 11837
MIM 600189
UniProt Q04724  
 TLE1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TLE1
Molecular Function
    transcription factor binding GO:0008134 IPI
    identical protein binding GO:0042802 IPI
    chromatin binding GO:0003682 IEA
    RNA polymerase II transcription corepressor activity GO:0001106 IDA
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    transcription, DNA-templated GO:0006351 IEA
    signal transduction GO:0007165 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    negative regulation of Wnt signaling pathway GO:0030178 NAS
    multicellular organismal development GO:0007275 TAS
    negative regulation of anoikis GO:2000811 IMP
    Notch signaling pathway GO:0007219 TAS
    organ morphogenesis GO:0009887 TAS
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IDA
    Wnt signaling pathway GO:0016055 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IMP
    nucleolus GO:0005730 IDA
    nucleus GO:0005634 IMP
    transcription factor complex GO:0005667 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified TLE1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TLE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZFP64  
Two-hybrid Homo sapiens
2 RELA 5970
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 DLEU1  
Two-hybrid Homo sapiens
4 SAT  
Two-hybrid Homo sapiens
5 MPHOSPH6 10200
Two-hybrid Homo sapiens
6 SNRPG 6637
Two-hybrid Homo sapiens
7 TK1 7083
Two-hybrid Homo sapiens
8 POLE2  
Two-hybrid Homo sapiens
9 LEF1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
10 HMGB1 3146
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
11 TNRC4  
Two-hybrid Homo sapiens
12 TLE2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 ERH 2079
Two-hybrid Homo sapiens
14 HSPE1 3336
Two-hybrid Homo sapiens
15 AES 166
Affinity Capture-MS Homo sapiens
16 GSTM4 2948
Two-hybrid Homo sapiens
17 RPA2 6118
Two-hybrid Homo sapiens
18 Hes1  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
19 CCL18  
Two-hybrid Homo sapiens
20 TLE1 7088
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PRDM1  
Invitro Homo sapiens
22 RUNX3  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
23 KDM6A  
Two-hybrid Homo sapiens
24 FXYD6  
Two-hybrid Homo sapiens
25 PXMP3  
Two-hybrid Homo sapiens
26 ARF4L  
Two-hybrid Homo sapiens
27 SIX1  
Affinity Capture-MS Homo sapiens
28 ATN1  
Two-hybrid Homo sapiens
29 BTBD2  
Two-hybrid Homo sapiens
30 CDK1 983
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
31 PSMD11 5717
Two-hybrid Homo sapiens
32 C1orf42  
Two-hybrid Homo sapiens
33 PFN1 5216
Two-hybrid Homo sapiens
34 IL6ST 3572
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
35 SIX3  
Invitro Homo sapiens
36 Foxg1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
37 CCNB1 891
Invitro Homo sapiens
38 ARL3 403
Two-hybrid Homo sapiens
39 HLA-DQA1 3117
Two-hybrid Homo sapiens
40 Tmsb4x  
Two-hybrid Mus musculus
41 FUBP1 8880
Two-hybrid Homo sapiens
42 RNF10  
Two-hybrid Homo sapiens
43 PAFAH1B3 5050
Two-hybrid Homo sapiens
44 RUNX1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
45 HES6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
46 PCDHA4 56144
Two-hybrid Homo sapiens
47 EIF2S2 8894
Two-hybrid Homo sapiens
48 UTY  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
49 SERPINB9 5272
Two-hybrid Homo sapiens
50 POLB 5423
Two-hybrid Homo sapiens
51 Morf4l1 21761
Affinity Capture-Western Mus musculus
52 CDKN2C  
Two-hybrid Homo sapiens
53 FOXA2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
54 TCF1  
Invitro Homo sapiens
View the network image/svg+xml



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