Gene description for POLB
Gene name polymerase (DNA directed), beta
Gene symbol POLB
Other names/aliases -
Species Homo sapiens
 Database cross references - POLB
ExoCarta ExoCarta_5423
Entrez Gene 5423
HGNC 9174
MIM 174760
UniProt P06746  
 POLB identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for POLB
Molecular Function
    microtubule binding GO:0008017 IDA
    lyase activity GO:0016829 IDA
    protein binding GO:0005515 IPI
    DNA-directed DNA polymerase activity GO:0003887 IDA
    metal ion binding GO:0046872 IEA
    damaged DNA binding GO:0003684 IEA
    enzyme binding GO:0019899 IPI
Biological Process
    pyrimidine dimer repair GO:0006290 IEA
    response to ethanol GO:0045471 IEA
    DNA repair GO:0006281 TAS
    base-excision repair GO:0006284 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 IMP
    cellular response to DNA damage stimulus GO:0006974 IMP
    DNA-dependent DNA replication GO:0006261 TAS
    base-excision repair, gap-filling GO:0006287 IEA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IEA
    DNA biosynthetic process GO:0071897 IEA
    neuron apoptotic process GO:0051402 IEA
Subcellular Localization
    protein complex GO:0043234 IDA
    microtubule GO:0005874 IDA
    spindle microtubule GO:0005876 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified POLB in exosomes
1
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MGC5306  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
2 NEIL1  
Far Western Homo sapiens
3 TLE1  
Two-hybrid Homo sapiens
4 MYST2  
Two-hybrid Homo sapiens
5 XRCC1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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