Gene description for WRN
Gene name Werner syndrome, RecQ helicase-like
Gene symbol WRN
Other names/aliases RECQ3
RECQL2
RECQL3
Species Homo sapiens
 Database cross references - WRN
ExoCarta ExoCarta_7486
Vesiclepedia VP_7486
Entrez Gene 7486
HGNC 12791
MIM 604611
UniProt Q14191  
 WRN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WRN
Molecular Function
    ATP binding GO:0005524 IEA
    ATPase activity GO:0016887 IDA
    magnesium ion binding GO:0000287 IDA
    G-quadruplex DNA binding GO:0051880 IDA
    3'-5' DNA helicase activity GO:0043138 IDA
    four-way junction helicase activity GO:0009378 IDA
    DNA binding GO:0003677 IDA
    protein complex binding GO:0032403 IDA
    ATP-dependent DNA helicase activity GO:0004003 IDA
    3'-5' exonuclease activity GO:0008408 IDA
    DNA helicase activity GO:0003678 IMP
    protein binding GO:0005515 IPI
    bubble DNA binding GO:0000405 IDA
    helicase activity GO:0004386 IDA
    ATP-dependent 3'-5' DNA helicase activity GO:0043140 IEA
    exonuclease activity GO:0004527 IDA
    Y-form DNA binding GO:0000403 IDA
    protein homodimerization activity GO:0042803 IDA
    manganese ion binding GO:0030145 IDA
Biological Process
    DNA metabolic process GO:0006259 IDA
    DNA synthesis involved in DNA repair GO:0000731 IDA
    response to UV-C GO:0010225 IDA
    response to oxidative stress GO:0006979 IDA
    DNA duplex unwinding GO:0032508 IMP
    nucleic acid phosphodiester bond hydrolysis GO:0090305 IEA
    nucleolus to nucleoplasm transport GO:0032066 IDA
    cell aging GO:0007569 IMP
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of hydrolase activity GO:0051345 IDA
    regulation of growth rate GO:0040009 IEA
    protein sumoylation GO:0016925 TAS
    DNA recombination GO:0006310 IEA
    telomere maintenance GO:0000723 IMP
    cellular response to gamma radiation GO:0071480 IDA
    replication fork processing GO:0031297 IMP
    cellular response to starvation GO:0009267 IDA
    double-strand break repair GO:0006302 IMP
    regulation of apoptotic process GO:0042981 IGI
    aging GO:0007568 NAS
    replicative cell aging GO:0001302 IEA
    DNA replication GO:0006260 IMP
    base-excision repair GO:0006284 IDA
    multicellular organismal aging GO:0010259 IMP
    cellular response to DNA damage stimulus GO:0006974 IDA
Subcellular Localization
    nucleolus GO:0005730 IDA
    nucleoplasm GO:0005654 TAS
    centrosome GO:0005813 IDA
    MutLalpha complex GO:0032389 IDA
 Experiment description of studies that identified WRN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WRN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLM 641
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 ATR  
Protein-peptide Homo sapiens
3 POLR1C 9533
Affinity Capture-Western Homo sapiens
4 PCNA 5111
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 XRCC6 2547
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 TERF2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
7 ATM 472
Protein-peptide Homo sapiens
8 XRCC5 7520
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 MDC1  
Protein-peptide Homo sapiens
10 TP53 7157
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 FEN1 2237
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
12 GIS1  
Two-hybrid 4932
13 WRNIP1 56897
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 PRKDC 5591
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which WRN is involved
PathwayEvidenceSource
SUMOylation of DNA damage response and repair proteins TAS Reactome





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