Gene description for CDKN2A
Gene name cyclin-dependent kinase inhibitor 2A
Gene symbol CDKN2A
Other names/aliases ARF
CDK4I
CDKN2
CMM2
INK4
INK4A
MLM
MTS-1
MTS1
P14
P14ARF
P16
P16-INK4A
P16INK4
P16INK4A
P19
P19ARF
TP16
Species Homo sapiens
 Database cross references - CDKN2A
ExoCarta ExoCarta_1029
Vesiclepedia VP_1029
Entrez Gene 1029
HGNC 1787
MIM 600160
UniProt Q8N726   P42771  
 CDKN2A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CDKN2A
Molecular Function
    transcription factor binding GO:0008134 IPI
    NF-kappaB binding GO:0051059 IDA
    ubiquitin-protein transferase inhibitor activity GO:0055105 ISS
    cyclin-dependent protein serine/threonine kinase inhibitor activity GO:0004861 IDA
    MDM2/MDM4 family protein binding GO:0097371 IPI
    protein kinase binding GO:0019901 IPI
    poly(A) RNA binding GO:0044822 IDA
    p53 binding GO:0002039 IPI
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
Biological Process
    activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0006919 IMP
    mitotic cell cycle GO:0000278 TAS
    Ras protein signal transduction GO:0007265 IEP
    negative regulation of ubiquitin-protein transferase activity GO:0051444 ISS
    senescence-associated heterochromatin focus assembly GO:0035986 IMP
    negative regulation of immature T cell proliferation in thymus GO:0033088 ISS
    regulation of apoptotic DNA fragmentation GO:1902510 IMP
    regulation of protein stability GO:0031647 ISS
    negative regulation of phosphorylation GO:0042326 IDA
    positive regulation of protein sumoylation GO:0033235 IMP
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    regulation of protein export from nucleus GO:0046825 IMP
    mitochondrial depolarization GO:0051882 IMP
    regulation of protein targeting to mitochondrion GO:1903214 IMP
    negative regulation of cell growth GO:0030308 IDA
    positive regulation of macrophage apoptotic process GO:2000111 ISS
    protein sumoylation GO:0016925 TAS
    regulation of G2/M transition of mitotic cell cycle GO:0010389 IMP
    positive regulation of cellular senescence GO:2000774 IMP
    protein K63-linked ubiquitination GO:0070534 IDA
    rRNA processing GO:0006364 IEA
    positive regulation of smooth muscle cell apoptotic process GO:0034393 ISS
    replicative senescence GO:0090399 IMP
    negative regulation of B cell proliferation GO:0030889 ISS
    negative regulation of proteolysis involved in cellular protein catabolic process GO:1903051 IMP
    positive regulation of cell cycle arrest GO:0071158 IDA
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of protein localization to nucleus GO:1900182 IDA
    protein polyubiquitination GO:0000209 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    somatic stem cell division GO:0048103 ISS
    negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:2000059 IDA
    negative regulation of cell-matrix adhesion GO:0001953 IMP
    negative regulation of protein kinase activity GO:0006469 IMP
    negative regulation of cell proliferation GO:0008285 IMP
    cellular senescence GO:0090398 IMP
    mitochondrion degradation GO:0000422 IMP
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    G1/S transition of mitotic cell cycle GO:0000082 IDA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of DNA damage response, signal transduction by p53 class mediator GO:0043517 IDA
    protein destabilization GO:0031648 IDA
    positive regulation of signal transduction by p53 class mediator GO:1901798 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    protein stabilization GO:0050821 IDA
    apoptotic mitochondrial changes GO:0008637 IMP
    post-translational protein modification GO:0043687 TAS
    cell cycle arrest GO:0007050 IMP
    negative regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045736 IDA
Subcellular Localization
    senescence-associated heterochromatin focus GO:0035985 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nuclear body GO:0016604 IDA
    mitochondrion GO:0005739 IMP
    protein complex GO:0043234 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified CDKN2A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CDKN2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CCND2  
Reconstituted Complex Homo sapiens
3 E4F1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
4 AURKA 6790
Affinity Capture-MS Homo sapiens
5 COPG2 26958
Invivo Homo sapiens
6 COPB1 1315
Invitro Homo sapiens
Invivo Homo sapiens
7 UBE2A  
Affinity Capture-Western Homo sapiens
8 CCNG1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 SERTAD1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PPP1R9B 84687
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 FKBP2 2286
Affinity Capture-Western Homo sapiens
13 CDK4 1019
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
14 MDM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 CDK6 1021
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
16 PLEKHA8  
Reconstituted Complex Homo sapiens
17 CCND1 595
Reconstituted Complex Homo sapiens
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