Gene description for CCND1
Gene name cyclin D1
Gene symbol CCND1
Other names/aliases BCL1
D11S287E
PRAD1
U21B31
Species Homo sapiens
 Database cross references - CCND1
ExoCarta ExoCarta_595
Vesiclepedia VP_595
Entrez Gene 595
HGNC 1582
MIM 168461
UniProt P24385  
 CCND1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CCND1
Molecular Function
    protein binding GO:0005515 IPI
    histone deacetylase binding GO:0042826 IPI
    protein kinase binding GO:0019901 IPI
    transcription corepressor activity GO:0003714 IDA
    cyclin-dependent protein serine/threonine kinase regulator activity GO:0016538 IEA
    proline-rich region binding GO:0070064 IDA
    transcription factor binding GO:0008134 IPI
    enzyme binding GO:0019899 IPI
    protein kinase activity GO:0004672 IEA
    protein complex binding GO:0032403 IEA
Biological Process
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IDA
    fat cell differentiation GO:0045444 IEA
    Leydig cell differentiation GO:0033327 IEA
    cell division GO:0051301 IEA
    mammary gland epithelial cell proliferation GO:0033598 IEA
    chromatin organization GO:0006325 TAS
    canonical Wnt signaling pathway GO:0060070 IEA
    mitotic G1 DNA damage checkpoint GO:0031571 IDA
    response to corticosterone GO:0051412 IEA
    negative regulation of epithelial cell differentiation GO:0030857 IEA
    lactation GO:0007595 IEA
    response to organonitrogen compound GO:0010243 IEA
    mammary gland alveolus development GO:0060749 IEA
    negative regulation of Wnt signaling pathway GO:0030178 IEA
    re-entry into mitotic cell cycle GO:0000320 IEA
    mitotic cell cycle GO:0000278 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    liver development GO:0001889 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    response to vitamin E GO:0033197 IEA
    positive regulation of mammary gland epithelial cell proliferation GO:0033601 IEA
    protein phosphorylation GO:0006468 IEA
    endoplasmic reticulum unfolded protein response GO:0030968 IEA
    negative regulation of cell cycle arrest GO:0071157 IDA
    organ regeneration GO:0031100 IEA
    response to ethanol GO:0045471 IEA
    response to X-ray GO:0010165 IEA
    positive regulation of protein phosphorylation GO:0001934 IDA
    response to drug GO:0042493 IEP
    cellular response to DNA damage stimulus GO:0006974 IDA
    response to estrogen GO:0043627 IEA
    Notch signaling pathway GO:0007219 TAS
    response to calcium ion GO:0051592 IEA
    response to magnesium ion GO:0032026 IEA
    response to UV-A GO:0070141 IDA
    transcription, DNA-templated GO:0006351 IEA
    response to iron ion GO:0010039 IEA
    positive regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045737 IDA
Subcellular Localization
    bicellular tight junction GO:0005923 IEA
    nucleoplasm GO:0005654 TAS
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IDA
    transcriptional repressor complex GO:0017053 IDA
    intracellular GO:0005622 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    membrane GO:0016020 IEA
 Experiment description of studies that identified CCND1 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CCND1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IFI27  
Invivo Homo sapiens
2 RBL1  
Invivo Homo sapiens
3 CCNDBP1 23582
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 ESR1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
5 TSC2 7249
Affinity Capture-Western Homo sapiens
6 THRB 7068
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 NCOA1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 RB1 5925
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 CTNNB1 1499
Invivo Homo sapiens
10 BRCA1 672
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 RBX1 9978
Affinity Capture-Western Homo sapiens
12 PCAF  
Affinity Capture-Western Homo sapiens
13 CALM1 801
Invivo Homo sapiens
14 DMTF1  
Invivo Homo sapiens
15 CDK6 1021
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 TAF1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 RFC1 5981
Affinity Capture-Western Homo sapiens
18 THRA  
Affinity Capture-Western Homo sapiens
19 CDKN1A  
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
20 AKAP8  
Affinity Capture-Western Homo sapiens
21 HDAC3 8841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 CDK4 1019
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 NPDC1  
Reconstituted Complex Homo sapiens
24 MYBL2 4605
Invitro Homo sapiens
Invivo Homo sapiens
25 EP300 2033
Reconstituted Complex Homo sapiens
26 NEUROD1  
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
27 STAT3 6774
Affinity Capture-Western Homo sapiens
28 AR 367
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
29 PCNA 5111
Reconstituted Complex Homo sapiens
30 CDKN2D  
Invivo Homo sapiens
31 CDKN1B 1027
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
32 NCOA3  
Affinity Capture-Western Homo sapiens
33 CDKN1C  
Reconstituted Complex Homo sapiens
34 CDKN2A 1029
Reconstituted Complex Homo sapiens
35 HERC5 51191
Reconstituted Complex Homo sapiens
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