Gene description for BTD
Gene name biotinidase
Gene symbol BTD
Other names/aliases -
Species Homo sapiens
 Database cross references - BTD
ExoCarta ExoCarta_686
Entrez Gene 686
HGNC 1122
MIM 609019
UniProt P43251  
 BTD identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for BTD
Molecular Function
    biotinidase activity GO:0047708 IEA
    biotin carboxylase activity GO:0004075 TAS
Biological Process
    water-soluble vitamin metabolic process GO:0006767 TAS
    small molecule metabolic process GO:0044281 TAS
    epidermis development GO:0008544 TAS
    biotin metabolic process GO:0006768 TAS
    vitamin metabolic process GO:0006766 TAS
    central nervous system development GO:0007417 TAS
Subcellular Localization
    perikaryon GO:0043204 IEA
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
    mitochondrial matrix GO:0005759 TAS
    nucleolus GO:0005730 IEA
    extracellular region GO:0005576 TAS
    apical part of cell GO:0045177 IEA
 Experiment description of studies that identified BTD in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for BTD
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which BTD is involved
PathwayEvidenceSource
Biotin transport and metabolism TAS Reactome
Defective BTD causes biotidinase deficiency TAS Reactome





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