Gene description for BTD
Gene name biotinidase
Gene symbol BTD
Other names/aliases -
Species Homo sapiens
 Database cross references - BTD
ExoCarta ExoCarta_686
Vesiclepedia VP_686
Entrez Gene 686
HGNC 1122
MIM 609019
UniProt P43251  
 BTD identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for BTD
Molecular Function
    protein binding GO:0005515 IPI
    biotinidase activity GO:0047708 TAS
Biological Process
    biotin metabolic process GO:0006768 IBA
    biotin metabolic process GO:0006768 TAS
    central nervous system development GO:0007417 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    mitochondrial matrix GO:0005759 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified BTD in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for BTD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 GPIHBP1  
Affinity Capture-MS Homo sapiens
3 KIF21B 23046
Affinity Capture-MS Homo sapiens
4 CST5 1473
Affinity Capture-MS Homo sapiens
5 MSH2 4436
Co-fractionation Homo sapiens
6 G6PD 2539
Co-fractionation Homo sapiens
7 SIKE1  
Affinity Capture-MS Homo sapiens
8 TAZ  
Affinity Capture-MS Homo sapiens
9 TMEM25  
Affinity Capture-MS Homo sapiens
10 UBA1 7317
Co-fractionation Homo sapiens
11 CD2AP 23607
Co-fractionation Homo sapiens
12 RBX1 9978
Co-fractionation Homo sapiens
13 SPX  
Affinity Capture-MS Homo sapiens
14 UCN3  
Affinity Capture-MS Homo sapiens
15 UBC 7316
Reconstituted Complex Homo sapiens
16 PPM1H  
Co-fractionation Homo sapiens
17 FBXO6 26270
Affinity Capture-MS Homo sapiens
18 SCIN 85477
Co-fractionation Homo sapiens
19 ERP44 23071
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TARDBP 23435
Co-fractionation Homo sapiens
21 MYO1D 4642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SERPINC1 462
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FBXO2 26232
Affinity Capture-MS Homo sapiens
24 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DHFRL1  
Affinity Capture-MS Homo sapiens
26 FAM19A2 338811
Affinity Capture-MS Homo sapiens
27 GPHA2  
Affinity Capture-MS Homo sapiens
28 CCND1 595
Affinity Capture-MS Homo sapiens
29 NMS  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here