ExoCarta: Gene summary

Credits | Contact

Gene description for KIF21B
Gene name	kinesin family member 21B
Gene symbol	KIF21B
Other names/aliases	-
Species	Homo sapiens
Database cross references - KIF21B
ExoCarta	ExoCarta_23046
Vesiclepedia	VP_23046
Entrez Gene	23046
HGNC	29442
MIM	608322
UniProt	O75037
KIF21B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Hepatocytes	26054723

Gene ontology annotations for KIF21B

Molecular Function
	microtubule motor activity	GO:0003777	IBA
	protein binding	GO:0005515	IPI
	ATP binding	GO:0005524	IEA
	microtubule binding	GO:0008017	IBA
	ATP hydrolysis activity	GO:0016887	IBA
Biological Process
	microtubule-based movement	GO:0007018	IBA
Subcellular Localization
	cytoplasm	GO:0005737	IBA
	kinesin complex	GO:0005871	IBA
	microtubule	GO:0005874	IBA
	dendrite	GO:0030425	IEA
	growth cone	GO:0030426	IEA
	cytoplasmic vesicle	GO:0031410	IEA

Experiment description of studies that identified KIF21B in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

237

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|HSC70\|GAPDH	Enriched markers
✔ HSP90B1	Negative markers
✔ qNano	Particle analysis

Identified molecule

mRNA

Identification method

RNA Sequencing

PubMed ID

26054723

Organism

Homo sapiens

Experiment description

Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs

Authors

"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"

Journal name

Carcinogenesis

Publication year

2015

Sample

Hepatocytes

Sample name

MIHA

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.19 g/mL

Molecules identified in the study

Protein
RNA

Methods used in the study

Western blotting
Mass spectrometry
RNA Sequencing

Protein-protein interactions for KIF21B

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

YWHAH

7533

Affinity Capture-MS

Homo sapiens

P3H1

64175

Affinity Capture-MS

Homo sapiens

TBX3

6926

Affinity Capture-MS

Homo sapiens

TAF5

Affinity Capture-MS

Homo sapiens

USF1

Affinity Capture-MS

Homo sapiens

GAN

8139

Affinity Capture-MS

Homo sapiens

TAF12

Affinity Capture-MS

Homo sapiens

PFDN6

10471

Affinity Capture-MS

Homo sapiens

UBL4A

8266

Affinity Capture-MS

Homo sapiens

KIF21A

55605

Cross-Linking-MS (XL-MS)		Homo sapiens
Affinity Capture-MS		Homo sapiens

TK1

7083

Two-hybrid

Homo sapiens

MED4

29079

Affinity Capture-MS

Homo sapiens

DMRTB1

Two-hybrid

Homo sapiens

YWHAB

7529

Affinity Capture-MS

Homo sapiens

TMEM248

55069

Affinity Capture-MS

Homo sapiens

PRPF6

24148

Affinity Capture-MS

Homo sapiens

HAUS2

Affinity Capture-MS

Homo sapiens

POLE3

Affinity Capture-MS

Homo sapiens

NUDT21

11051

Affinity Capture-MS

Homo sapiens

Oxnad1

Affinity Capture-MS

Mus musculus

TUBB4B

10383

Affinity Capture-MS

Homo sapiens

UBC

7316

Affinity Capture-MS

Homo sapiens

FUBP3

8939

Affinity Capture-MS

Homo sapiens

ZNF281

Affinity Capture-MS

Homo sapiens

CSTF2

1478

Affinity Capture-MS

Homo sapiens

PMF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BAG6

7917

Affinity Capture-MS

Homo sapiens

CAPG

822

Affinity Capture-MS

Homo sapiens

BAG1

573

Affinity Capture-MS

Homo sapiens

WDYHV1

Two-hybrid

Homo sapiens

COL4A5

Affinity Capture-MS

Homo sapiens

BTD

686

Affinity Capture-MS

Homo sapiens

TRIM3

10612

Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens

SYNCRIP

10492

Affinity Capture-MS

Homo sapiens

WDR75

Affinity Capture-MS

Homo sapiens

LDLR

3949

Positive Genetic

Homo sapiens

YWHAQ

10971

Affinity Capture-MS

Homo sapiens

QKI

9444

Two-hybrid

Homo sapiens

YWHAG

7532

Affinity Capture-MS

Homo sapiens

CPSF1

29894

Affinity Capture-MS

Homo sapiens

CPSF3

51692

Affinity Capture-MS

Homo sapiens

TICAM1

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which KIF21B is involved

Pathway	Evidence	Source
COPI-dependent Golgi-to-ER retrograde traffic	TAS	Reactome
Factors involved in megakaryocyte development and platelet production	TAS	Reactome
Golgi-to-ER retrograde transport	TAS	Reactome
Hemostasis	TAS	Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic	TAS	Reactome
Kinesins	TAS	Reactome
Membrane Trafficking	TAS	Reactome
Vesicle-mediated transport	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here