Gene description for GAN
Gene name gigaxonin
Gene symbol GAN
Other names/aliases GAN1
KLHL16
Species Homo sapiens
 Database cross references - GAN
ExoCarta ExoCarta_8139
Vesiclepedia VP_8139
Entrez Gene 8139
HGNC 4137
MIM 605379
UniProt Q9H2C0  
 GAN identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GAN
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IBA
Biological Process
    cytoskeleton organization GO:0007010 IEA
    protein ubiquitination GO:0016567 IDA
    protein ubiquitination GO:0016567 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    Cul3-RING ubiquitin ligase complex GO:0031463 IBA
    Cul3-RING ubiquitin ligase complex GO:0031463 IDA
 Experiment description of studies that identified GAN in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GAN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 RPSA 3921
Affinity Capture-MS Homo sapiens
4 KRT37 8688
Affinity Capture-MS Homo sapiens
5 KRT73 319101
Affinity Capture-MS Homo sapiens
6 TUBA8 51807
Affinity Capture-MS Homo sapiens
7 CCT3 7203
Affinity Capture-MS Homo sapiens
8 KRT6B 3854
Affinity Capture-MS Homo sapiens
9 KRT1 3848
Affinity Capture-MS Homo sapiens
10 LNP1  
Affinity Capture-MS Homo sapiens
11 APBB2  
Affinity Capture-MS Homo sapiens
12 EIF3C 8663
Affinity Capture-MS Homo sapiens
13 RPS4X 6191
Affinity Capture-MS Homo sapiens
14 KIFC3 3801
Affinity Capture-MS Homo sapiens
15 OXA1L  
Affinity Capture-MS Homo sapiens
16 ZNHIT2 741
Affinity Capture-MS Homo sapiens
17 UBA1 7317
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 TUBB8 347688
Affinity Capture-MS Homo sapiens
19 HSPA1L 3305
Affinity Capture-MS Homo sapiens
20 PRPH 5630
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
21 HNRNPC 3183
Affinity Capture-MS Homo sapiens
22 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
23 CCT4 10575
Affinity Capture-MS Homo sapiens
24 SYNE2 23224
Affinity Capture-MS Homo sapiens
25 CCT6B 10693
Affinity Capture-MS Homo sapiens
26 CFAP44 55779
Affinity Capture-MS Homo sapiens
27 DLG5 9231
Affinity Capture-MS Homo sapiens
28 CBY1  
Affinity Capture-MS Homo sapiens
29 DNAH8 1769
Affinity Capture-MS Homo sapiens
30 MAP4K3 8491
Affinity Capture-MS Homo sapiens
31 TAF8  
Affinity Capture-MS Homo sapiens
32 ZNF618  
Affinity Capture-MS Homo sapiens
33 NUP160 23279
Affinity Capture-MS Homo sapiens
34 RPS3 6188
Affinity Capture-MS Homo sapiens
35 POTEF 728378
Affinity Capture-MS Homo sapiens
36 FAM126A 84668
Affinity Capture-MS Homo sapiens
37 S100A4 6275
Affinity Capture-MS Homo sapiens
38 WDR76  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RDX 5962
Affinity Capture-MS Homo sapiens
40 TBCB 1155
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
41 HSPA2 3306
Affinity Capture-MS Homo sapiens
42 LRMP  
Affinity Capture-MS Homo sapiens
43 HELZ 9931
Affinity Capture-MS Homo sapiens
44 SLC13A2 9058
Affinity Capture-MS Homo sapiens
45 SPIN1  
Affinity Capture-MS Homo sapiens
46 EIF3B 8662
Affinity Capture-MS Homo sapiens
47 CUX1 1523
Affinity Capture-MS Homo sapiens
48 NPM1 4869
Affinity Capture-MS Homo sapiens
49 DMD 1756
Affinity Capture-MS Homo sapiens
50 RPS9 6203
Affinity Capture-MS Homo sapiens
51 MRPS26 64949
Affinity Capture-MS Homo sapiens
52 KRT31 3881
Affinity Capture-MS Homo sapiens
53 ANKRD36 375248
Affinity Capture-MS Homo sapiens
54 SCN5A 6331
Affinity Capture-MS Homo sapiens
55 DNHD1  
Affinity Capture-MS Homo sapiens
56 ATG16L1 55054
Affinity Capture-MS Homo sapiens
57 SFN 2810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HSPA6 3310
Affinity Capture-MS Homo sapiens
59 CCT7 10574
Affinity Capture-MS Homo sapiens
60 PARP14 54625
Affinity Capture-MS Homo sapiens
61 CCDC73  
Affinity Capture-MS Homo sapiens
62 CTU1 90353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 PABPC4 8761
Affinity Capture-MS Homo sapiens
64 HSPD1 3329
Affinity Capture-MS Homo sapiens
65 ATAD3A 55210
Affinity Capture-MS Homo sapiens
66 HSPA8 3312
Affinity Capture-MS Homo sapiens
67 PLCB1 23236
Affinity Capture-MS Homo sapiens
68 TRRAP 8295
Affinity Capture-MS Homo sapiens
69 CCT2 10576
Affinity Capture-MS Homo sapiens
70 CPEB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ALMS1  
Affinity Capture-MS Homo sapiens
72 ZMYM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 MYO18A 399687
Affinity Capture-MS Homo sapiens
74 TCP1 6950
Affinity Capture-MS Homo sapiens
75 SPTB 6710
Affinity Capture-MS Homo sapiens
76 SPTA1  
Affinity Capture-MS Homo sapiens
77 EIF4A1 1973
Affinity Capture-MS Homo sapiens
78 RBX1 9978
Affinity Capture-Western Homo sapiens
79 EPX 8288
Affinity Capture-MS Homo sapiens
80 RPL4 6124
Affinity Capture-MS Homo sapiens
81 ZNF469  
Affinity Capture-MS Homo sapiens
82 MAP1B 4131
Affinity Capture-Western Homo sapiens
83 RPS10 6204
Affinity Capture-MS Homo sapiens
84 SAMD4A 23034
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 TUBA1A 7846
Affinity Capture-MS Homo sapiens
86 CCT5 22948
Affinity Capture-MS Homo sapiens
87 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
88 RNF213 57674
Affinity Capture-MS Homo sapiens
89 TUBB2B 347733
Affinity Capture-MS Homo sapiens
90 PABPC1 26986
Affinity Capture-MS Homo sapiens
91 TUBA1B 10376
Affinity Capture-MS Homo sapiens
92 HAUS8  
Affinity Capture-MS Homo sapiens
93 TRIM21 6737
Affinity Capture-MS Homo sapiens
94 GFAP 2670
Affinity Capture-MS Homo sapiens
95 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 KRT75 9119
Affinity Capture-MS Homo sapiens
97 SYNE1 23345
Affinity Capture-MS Homo sapiens
98 MUC5AC 4586
Affinity Capture-MS Homo sapiens
99 KRT14 3861
Affinity Capture-MS Homo sapiens
100 KIF11 3832
Affinity Capture-MS Homo sapiens
101 RPS6 6194
Affinity Capture-MS Homo sapiens
102 ARHGEF12 23365
Affinity Capture-MS Homo sapiens
103 SLC25A4 291
Affinity Capture-MS Homo sapiens
104 PAK7  
Affinity Capture-MS Homo sapiens
105 UPK1A 11045
Affinity Capture-MS Homo sapiens
106 NINL  
Affinity Capture-MS Homo sapiens
107 CEP72  
Affinity Capture-MS Homo sapiens
108 PRPF31 26121
Affinity Capture-MS Homo sapiens
109 ACTG1 71
Affinity Capture-MS Homo sapiens
110 TENM3 55714
Affinity Capture-MS Homo sapiens
111 KBTBD8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 NUDCD3 23386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 SLC25A5 292
Affinity Capture-MS Homo sapiens
114 RBM10  
Affinity Capture-MS Homo sapiens
115 RPLP0 6175
Affinity Capture-MS Homo sapiens
116 SEMA5B  
Affinity Capture-MS Homo sapiens
117 BCLAF1 9774
Affinity Capture-MS Homo sapiens
118 TTN 7273
Affinity Capture-MS Homo sapiens
119 DST 667
Affinity Capture-MS Homo sapiens
120 TEKT1  
Affinity Capture-MS Homo sapiens
121 CTNNA2 1496
Affinity Capture-MS Homo sapiens
122 GAN 8139
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
123 TUBB 203068
Affinity Capture-MS Homo sapiens
124 GPR101  
Affinity Capture-MS Homo sapiens
125 ACTBL2 345651
Affinity Capture-MS Homo sapiens
126 KRT33B 3884
Affinity Capture-MS Homo sapiens
127 RPS2 6187
Affinity Capture-MS Homo sapiens
128 TMOD4  
Affinity Capture-MS Homo sapiens
129 DES 1674
Affinity Capture-MS Homo sapiens
130 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
131 CCT6A 908
Affinity Capture-MS Homo sapiens
132 KRT32  
Affinity Capture-MS Homo sapiens
133 EEF2 1938
Affinity Capture-MS Homo sapiens
134 MYH14 79784
Affinity Capture-MS Homo sapiens
135 MXRA5 25878
Affinity Capture-MS Homo sapiens
136 LMNB2 84823
Affinity Capture-MS Homo sapiens
137 PRDX1 5052
Affinity Capture-MS Homo sapiens
138 VCPIP1 80124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
139 SETD2  
Affinity Capture-MS Homo sapiens
140 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
141 YWHAZ 7534
Affinity Capture-MS Homo sapiens
142 TUBB4B 10383
Affinity Capture-MS Homo sapiens
143 PRKD1 5587
Affinity Capture-MS Homo sapiens
144 ZC3HC1  
Affinity Capture-MS Homo sapiens
145 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
146 PKD1 5310
Affinity Capture-MS Homo sapiens
147 KIAA1524 57650
Affinity Capture-MS Homo sapiens
148 ANKRD36B  
Affinity Capture-MS Homo sapiens
149 WIPF1 7456
Affinity Capture-MS Homo sapiens
150 KRT82 3888
Affinity Capture-MS Homo sapiens
151 KRT27 342574
Affinity Capture-MS Homo sapiens
152 KIF21B 23046
Affinity Capture-MS Homo sapiens
153 PRMT5 10419
Affinity Capture-MS Homo sapiens
154 INPP5K 51763
Affinity Capture-MS Homo sapiens
155 INA 9118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 NEFL 4747
Affinity Capture-MS Homo sapiens
157 NEFM 4741
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 RPA3 6119
Proximity Label-MS Homo sapiens
159 RPS13 6207
Affinity Capture-MS Homo sapiens
160 CELSR3  
Affinity Capture-MS Homo sapiens
161 IGSF3 3321
Affinity Capture-MS Homo sapiens
162 HSPA1A 3303
Affinity Capture-MS Homo sapiens
163 DNAJC10 54431
Affinity Capture-MS Homo sapiens
164 KRT84 3890
Affinity Capture-MS Homo sapiens
165 PHLDB1 23187
Affinity Capture-MS Homo sapiens
166 CROCC 9696
Affinity Capture-MS Homo sapiens
167 C11orf84  
Affinity Capture-MS Homo sapiens
168 HSPA9 3313
Affinity Capture-MS Homo sapiens
169 PABPC3 5042
Affinity Capture-MS Homo sapiens
170 KRT16 3868
Affinity Capture-MS Homo sapiens
171 LRRC16A 55604
Affinity Capture-MS Homo sapiens
172 EIF3D 8664
Affinity Capture-MS Homo sapiens
173 ZC3H13 23091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
174 H2AFX 3014
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 QRICH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
176 RPL13 6137
Affinity Capture-MS Homo sapiens
177 RELA 5970
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
178 DNAH6 1768
Affinity Capture-MS Homo sapiens
179 RTTN  
Affinity Capture-MS Homo sapiens
180 RPS27A 6233
Affinity Capture-MS Homo sapiens
181 CHML  
Co-fractionation Homo sapiens
182 SPTAN1 6709
Affinity Capture-MS Homo sapiens
183 CCT8 10694
Affinity Capture-MS Homo sapiens
184 SLC25A6 293
Affinity Capture-MS Homo sapiens
185 WDR77 79084
Affinity Capture-MS Homo sapiens
186 BAD  
Affinity Capture-MS Homo sapiens
187 PLEC 5339
Affinity Capture-MS Homo sapiens
188 EIF3I 8668
Affinity Capture-MS Homo sapiens
189 STK38 11329
Affinity Capture-MS Homo sapiens
190 KRT10 3858
Affinity Capture-MS Homo sapiens
191 DCDC1  
Affinity Capture-MS Homo sapiens
192 PLXNA1 5361
Affinity Capture-MS Homo sapiens
193 ZZEF1 23140
Affinity Capture-MS Homo sapiens
194 AJUBA  
Affinity Capture-MS Homo sapiens
195 HNRNPM 4670
Affinity Capture-MS Homo sapiens
196 TUBB3 10381
Affinity Capture-MS Homo sapiens
197 RIOK1 83732
Affinity Capture-MS Homo sapiens
198 THRAP3 9967
Affinity Capture-MS Homo sapiens
199 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
200 CLUH 23277
Co-fractionation Homo sapiens
201 PLOD1 5351
Affinity Capture-MS Homo sapiens
202 CSPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
203 CACNA1H  
Affinity Capture-MS Homo sapiens
204 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
205 MTHFD1 4522
Affinity Capture-MS Homo sapiens
206 RPS5 6193
Affinity Capture-MS Homo sapiens
207 TUBB4A 10382
Affinity Capture-MS Homo sapiens
208 TUBB6 84617
Affinity Capture-MS Homo sapiens
209 NEFH 4744
Affinity Capture-MS Homo sapiens
210 TRAP1 10131
Affinity Capture-MS Homo sapiens
211 SNRPN 6638
Affinity Capture-MS Homo sapiens
212 MAST2  
Affinity Capture-MS Homo sapiens
213 RPS3A 6189
Affinity Capture-MS Homo sapiens
214 CLASP1 23332
Affinity Capture-MS Homo sapiens
215 EEF1A1P5 158078
Affinity Capture-MS Homo sapiens
216 NCL 4691
Affinity Capture-MS Homo sapiens
217 KRT38 8687
Affinity Capture-MS Homo sapiens
218 EIF4B 1975
Affinity Capture-MS Homo sapiens
219 SRSF7 6432
Affinity Capture-MS Homo sapiens
220 PINK1  
Affinity Capture-MS Homo sapiens
221 GOT1 2805
Affinity Capture-MS Homo sapiens
222 RPS8 6202
Affinity Capture-MS Homo sapiens
223 RPL8 6132
Affinity Capture-MS Homo sapiens
224 HNRNPF 3185
Affinity Capture-MS Homo sapiens
225 ZNF616  
Affinity Capture-MS Homo sapiens
226 PGM3 5238
Affinity Capture-MS Homo sapiens
227 DNAH10 196385
Affinity Capture-MS Homo sapiens
228 EEFSEC 60678
Affinity Capture-MS Homo sapiens
229 PCNT  
Affinity Capture-MS Homo sapiens
230 HNRNPU 3192
Affinity Capture-MS Homo sapiens
231 GNB2L1 10399
Affinity Capture-MS Homo sapiens
232 OR2A4 79541
Affinity Capture-MS Homo sapiens
233 MRPL39 54148
Affinity Capture-MS Homo sapiens
234 VIM 7431
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
235 SSH2  
Affinity Capture-MS Homo sapiens
236 PRMT1 3276
Affinity Capture-MS Homo sapiens
237 KCTD5 54442
Affinity Capture-MS Homo sapiens
238 SCN1A  
Affinity Capture-MS Homo sapiens
239 COL6A3 1293
Affinity Capture-MS Homo sapiens
240 SPTBN5 51332
Affinity Capture-MS Homo sapiens
241 EIF3L 51386
Affinity Capture-MS Homo sapiens
242 CEBPZ  
Affinity Capture-MS Homo sapiens
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