Gene description for DNAH10
Gene name dynein, axonemal, heavy chain 10
Gene symbol DNAH10
Other names/aliases -
Species Homo sapiens
 Database cross references - DNAH10
ExoCarta ExoCarta_196385
Vesiclepedia VP_196385
Entrez Gene 196385
HGNC 2941
MIM 605884
UniProt Q8IVF4  
 DNAH10 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for DNAH10
Molecular Function
    ATP binding GO:0005524 IEA
    minus-end-directed microtubule motor activity GO:0008569 IBA
    ATP hydrolysis activity GO:0016887 IEA
    dynein intermediate chain binding GO:0045505 IBA
    dynein light intermediate chain binding GO:0051959 IBA
Biological Process
    cilium movement involved in cell motility GO:0060294 IBA
Subcellular Localization
    microtubule GO:0005874 IEA
    axoneme GO:0005930 IEA
    dynein complex GO:0030286 IBA
    9+2 motile cilium GO:0097729 IBA
 Experiment description of studies that identified DNAH10 in sEVs
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DNAH10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX46 9879
Cross-Linking-MS (XL-MS) Homo sapiens
2 PSMD14 10213
Affinity Capture-MS Homo sapiens
3 GATA4  
Affinity Capture-MS Homo sapiens
4 ACTR1A 10121
Proximity Label-MS Homo sapiens
5 GAN 8139
Affinity Capture-MS Homo sapiens
6 TBC1D32 221322
Proximity Label-MS Homo sapiens
7 CHD5 26038
Cross-Linking-MS (XL-MS) Homo sapiens
8 HSPD1 3329
Cross-Linking-MS (XL-MS) Homo sapiens
9 TP53 7157
Affinity Capture-MS Homo sapiens
10 DCTN2 10540
Proximity Label-MS Homo sapiens
11 ACLY 47
Cross-Linking-MS (XL-MS) Homo sapiens
12 NKX2-5  
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 HIST1H2AB 8335
Cross-Linking-MS (XL-MS) Homo sapiens
15 LINC00839  
Protein-RNA Homo sapiens
16 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which DNAH10 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here