Gene description for CHD5
Gene name chromodomain helicase DNA binding protein 5
Gene symbol CHD5
Other names/aliases CHD-5
Species Homo sapiens
 Database cross references - CHD5
ExoCarta ExoCarta_26038
Vesiclepedia VP_26038
Entrez Gene 26038
HGNC 16816
MIM 610771
UniProt Q8TDI0  
 CHD5 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for CHD5
Molecular Function
    H3K27me3 modified histone binding GO:0061628 IDA
    ATP binding GO:0005524 IEA
    DNA binding GO:0003677 IEA
    zinc ion binding GO:0008270 IEA
    ATP-dependent helicase activity GO:0008026 IEA
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 IEA
    histone H4 acetylation GO:0043967 ISS
    positive regulation of signal transduction by p53 class mediator GO:1901798 ISS
    cerebral cortex neuron differentiation GO:0021895 ISS
    histone H3-K27 trimethylation GO:0098532 IMP
    regulation of transcription involved in cell fate commitment GO:0060850 IMP
    negative regulation of cell proliferation GO:0008285 ISS
    spermatogenesis, exchange of chromosomal proteins GO:0035093 ISS
Subcellular Localization
    NuRD complex GO:0016581 ISS
    membrane GO:0016020 IDA
    heterochromatin GO:0000792 ISS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified CHD5 in exosomes
1
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which CHD5 is involved
No pathways found





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