Gene description for CHD3
Gene name chromodomain helicase DNA binding protein 3
Gene symbol CHD3
Other names/aliases Mi-2a
Mi2-ALPHA
ZFH
Species Homo sapiens
 Database cross references - CHD3
ExoCarta ExoCarta_1107
Vesiclepedia VP_1107
Entrez Gene 1107
HGNC 1918
MIM 602120
UniProt Q12873  
 CHD3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for CHD3
Molecular Function
    ATP-dependent DNA helicase activity GO:0004003 TAS
    ATP binding GO:0005524 IEA
    zinc ion binding GO:0008270 NAS
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
    helicase activity GO:0004386 NAS
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    chromatin modification GO:0016568 IEA
    chromatin organization GO:0006325 TAS
    chromatin assembly or disassembly GO:0006333 IEA
    spindle organization GO:0007051 IDA
    DNA duplex unwinding GO:0032508 TAS
    transcription, DNA-templated GO:0006351 IEA
    regulation of transcription, DNA-templated GO:0006355 NAS
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    centrosome organization GO:0051297 IDA
Subcellular Localization
    nucleus GO:0005634 NAS
    cytoplasm GO:0005737 IDA
    NuRD complex GO:0016581 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    centrosome GO:0005813 IDA
 Experiment description of studies that identified CHD3 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C7orf36  
Two-hybrid Homo sapiens
2 SERF2 10169
Two-hybrid Homo sapiens
3 PTN  
Two-hybrid Homo sapiens
4 IMMT 10989
Two-hybrid Homo sapiens
5 SAT  
Two-hybrid Homo sapiens
6 PRG2  
Two-hybrid Homo sapiens
7 ADH5 128
Two-hybrid Homo sapiens
8 KIF15 56992
Two-hybrid Homo sapiens
9 ATPIF1 93974
Two-hybrid Homo sapiens
10 HDAC2 3066
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
11 MTA2 9219
Co-purification Homo sapiens
12 CASP6 839
Two-hybrid Homo sapiens
13 TP53 7157
Two-hybrid Homo sapiens
14 SMARCA5 8467
Co-purification Homo sapiens
15 PCMT1 5110
Two-hybrid Homo sapiens
16 IVNS1ABP  
Two-hybrid Homo sapiens
17 SAFB 6294
Two-hybrid Homo sapiens
18 TTR 7276
Two-hybrid Homo sapiens
19 FUBP1 8880
Two-hybrid Homo sapiens
20 PAICS 10606
Two-hybrid Homo sapiens
21 SRRT 51593
Two-hybrid Homo sapiens
22 CASP8 841
Two-hybrid Homo sapiens
23 CTTN 2017
Two-hybrid Homo sapiens
24 THOC7 80145
Two-hybrid Homo sapiens
25 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 SLC27A6  
Two-hybrid Homo sapiens
27 MAFG  
Two-hybrid Homo sapiens
28 PUF60 22827
Two-hybrid Homo sapiens
29 SDCCAG3  
Two-hybrid Homo sapiens
30 CREB1  
Two-hybrid Homo sapiens
31 PSME1 5720
Two-hybrid Homo sapiens
32 TP73  
Two-hybrid Homo sapiens
33 TXNDC9 10190
Two-hybrid Homo sapiens
34 C1orf42  
Two-hybrid Homo sapiens
35 XRCC4  
Two-hybrid Homo sapiens
36 UBE2I 7329
Two-hybrid Homo sapiens
37 IK 3550
Two-hybrid Homo sapiens
38 TRIM28 10155
Two-hybrid Homo sapiens
39 UBA3 9039
Two-hybrid Homo sapiens
40 PTPRS 5802
Two-hybrid Homo sapiens
41 MAN2A2 4122
Two-hybrid Homo sapiens
42 TSPAN6 7105
Two-hybrid Homo sapiens
43 FAM134A 79137
Two-hybrid Homo sapiens
44 HABP4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
45 SUMO2 6613
Two-hybrid Homo sapiens
46 SIRT6  
Two-hybrid Homo sapiens
47 LUC7L2 51631
Two-hybrid Homo sapiens
48 SERBP1 26135
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
49 BHLHB2  
Two-hybrid Homo sapiens
50 MBD2 8932
Reconstituted Complex Homo sapiens
51 HSPH1 10808
Two-hybrid Homo sapiens
52 MBD3L1  
Affinity Capture-Western Homo sapiens
53 C4orf17  
Two-hybrid Homo sapiens
54 CSTF2 1478
Two-hybrid Homo sapiens
55 FBP2 8789
Two-hybrid Homo sapiens
56 RUTBC1  
Two-hybrid Homo sapiens
57 TNFRSF14  
Two-hybrid Homo sapiens
58 GPS2  
Two-hybrid Homo sapiens
59 RAD51  
Two-hybrid Homo sapiens
60 RAD21 5885
Co-purification Homo sapiens
61 SUMO1 7341
Two-hybrid Homo sapiens
62 CPE 1363
Two-hybrid Homo sapiens
63 RPL29 6159
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CHD3 is involved
PathwayEvidenceSource
HDACs deacetylate histones TAS Reactome
RNA Polymerase I Transcription Initiation TAS Reactome





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