Gene description for CHD6
Gene name chromodomain helicase DNA binding protein 6
Gene symbol CHD6
Other names/aliases CHD-6
CHD5
RIGB
Species Homo sapiens
 Database cross references - CHD6
ExoCarta ExoCarta_84181
Vesiclepedia VP_84181
Entrez Gene 84181
HGNC 19057
MIM 616114
UniProt Q8TD26  
 CHD6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for CHD6
Molecular Function
    transcription coregulator binding GO:0001221 IPI
    DNA binding GO:0003677 IBA
    chromatin binding GO:0003682 IBA
    DNA clamp loader activity GO:0003689 IEA
    ATP binding GO:0005524 IEA
    ATP-dependent activity, acting on DNA GO:0008094 IDA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IBA
    double-stranded DNA helicase activity GO:0036121 IEA
    histone binding GO:0042393 IBA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent chromatin remodeler activity GO:0140658 IBA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    chromatin remodeling GO:0006338 IBA
    regulation of gene expression GO:0010468 IBA
    DNA duplex unwinding GO:0032508 IEA
    cell redox homeostasis GO:0045454 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    chromatin looping GO:0140588 IEA
Subcellular Localization
    chromatin GO:0000785 IBA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified CHD6 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for CHD6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 TLX2  
Proximity Label-MS Homo sapiens
3 GUSBP5  
Affinity Capture-MS Homo sapiens
4 PAX6  
Proximity Label-MS Homo sapiens
5 ASF1A 25842
Proximity Label-MS Homo sapiens
6 PARP1 142
Proximity Label-MS Homo sapiens
7 PSMD14 10213
Affinity Capture-MS Homo sapiens
8 COIL  
Proximity Label-MS Homo sapiens
9 FEV  
Proximity Label-MS Homo sapiens
10 SOX5 6660
Proximity Label-MS Homo sapiens
11 NR3C1 2908
Proximity Label-MS Homo sapiens
12 RACGAP1 29127
Affinity Capture-MS Homo sapiens
13 ATXN1L  
Two-hybrid Homo sapiens
14 TOLLIP 54472
Two-hybrid Homo sapiens
15 GATA2  
Proximity Label-MS Homo sapiens
16 KLF3  
Proximity Label-MS Homo sapiens
17 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
18 TLX3  
Proximity Label-MS Homo sapiens
19 EGFR 1956
Affinity Capture-MS Homo sapiens
20 PAPD5 64282
Affinity Capture-MS Homo sapiens
21 HIST3H3 8290
Protein-peptide Homo sapiens
22 ELK3  
Proximity Label-MS Homo sapiens
23 CENPA  
Proximity Label-MS Homo sapiens
24 ESR1  
Affinity Capture-MS Homo sapiens
25 PAX7  
Proximity Label-MS Homo sapiens
26 AIRE  
Affinity Capture-MS Homo sapiens
27 SEC16A 9919
Affinity Capture-MS Homo sapiens
28 ZCCHC10  
Affinity Capture-MS Homo sapiens
29 CHD3 1107
Co-fractionation Homo sapiens
30 RPS6 6194
Cross-Linking-MS (XL-MS) Homo sapiens
31 ZFYVE9  
Two-hybrid Homo sapiens
32 ZFYVE1 53349
Affinity Capture-MS Homo sapiens
33 POLR1E  
Proximity Label-MS Homo sapiens
34 ARAF 369
Two-hybrid Homo sapiens
35 KLF12  
Proximity Label-MS Homo sapiens
36 MECP2 4204
Affinity Capture-MS Homo sapiens
37 VSX1  
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CHD6 is involved
No pathways found





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