 Experiment description of studies that identified HIST3H3 in exosomes |
| 1 |
| Experiment ID |
224 |
| ISEV standards |
|
✔
EM|AFM
|
EV Biophysical techniques |
|
✔
Alix|TSG101
|
EV Cytosolic markers |
|
✔
CD63|CD81
|
EV Membrane markers |
|
✔
GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 2 |
| Experiment ID |
138 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
|
✔
LAMP2|CD9
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 3 |
| Experiment ID |
143 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
|
✔
LAMP2|CD9
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
LNCaP - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 4 |
| Experiment ID |
196 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
|
✔
CD9
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22418980
|
| Organism |
Homo sapiens |
| Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
| Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
| Journal name |
Kidney Int
|
| Publication year |
2012 |
| Sample |
Urine |
| Sample name |
Urine - Normal high density |
| Isolation/purification methods |
Differential centrifugation
Sucrose cushion |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
197 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
Alix|TSG101|HSP70
|
EV Cytosolic markers |
|
✔
CD9
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22418980
|
| Organism |
Homo sapiens |
| Experiment description |
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes. |
| Authors |
Raj DA, Fiume I, Capasso G, Pocsfalvi G. |
| Journal name |
Kidney Int
|
| Publication year |
2012 |
| Sample |
Urine |
| Sample name |
Urine - Normal low density |
| Isolation/purification methods |
Differential centrifugation
Sucrose cushion |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
