Gene description for ATAD2
Gene name ATPase family, AAA domain containing 2
Gene symbol ATAD2
Other names/aliases ANCCA
CT137
PRO2000
Species Homo sapiens
 Database cross references - ATAD2
ExoCarta ExoCarta_29028
Entrez Gene 29028
HGNC 30123
MIM 611941
UniProt Q6PL18  
 ATAD2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for ATAD2
Molecular Function
    chromatin binding GO:0003682 IBA
    histone binding GO:0042393 IBA
    ATP binding GO:0005524 IEA
    ATPase activity GO:0016887 IDA
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IBA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of chromatin silencing GO:0031936 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IBA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified ATAD2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATAD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ATAD2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here