Gene description for SPTBN2
Gene name spectrin, beta, non-erythrocytic 2
Gene symbol SPTBN2
Other names/aliases GTRAP41
SCA5
SCAR14
Species Homo sapiens
 Database cross references - SPTBN2
ExoCarta ExoCarta_6712
Entrez Gene 6712
HGNC 11276
MIM 604985
UniProt O15020  
 SPTBN2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SPTBN2
Molecular Function
    actin binding GO:0003779 IEA
    phospholipid binding GO:0005543 IEA
    structural constituent of cytoskeleton GO:0005200 TAS
Biological Process
    axon guidance GO:0007411 TAS
    actin filament capping GO:0051693 IEA
    multicellular organism growth GO:0035264 IEA
    vesicle-mediated transport GO:0016192 IDA
    cerebellar Purkinje cell layer morphogenesis GO:0021692 IEA
    adult behavior GO:0030534 IEA
    synapse assembly GO:0007416 IEA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cell junction GO:0030054 IDA
    neuronal cell body GO:0043025 IEA
    cytosol GO:0005829 TAS
    extracellular space GO:0005615 IDA
    spectrin GO:0008091 IDA
    apical plasma membrane GO:0016324 IEA
 Experiment description of studies that identified SPTBN2 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPTBN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UNC13B 10497
Two-hybrid Homo sapiens
2 DCTN1 1639
Invivo Homo sapiens
3 ACTR1A 10121
Invivo Homo sapiens
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