Gene description for SPTBN2
Gene name spectrin, beta, non-erythrocytic 2
Gene symbol SPTBN2
Other names/aliases GTRAP41
SCA5
SCAR14
Species Homo sapiens
 Database cross references - SPTBN2
ExoCarta ExoCarta_6712
Vesiclepedia VP_6712
Entrez Gene 6712
HGNC 11276
MIM 604985
UniProt O15020  
 SPTBN2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SPTBN2
Molecular Function
    actin binding GO:0003779 TAS
    structural constituent of cytoskeleton GO:0005200 TAS
    phospholipid binding GO:0005543 IEA
    cadherin binding GO:0045296 HDA
    actin filament binding GO:0051015 IBA
    structural constituent of synapse GO:0098918 IBA
    structural constituent of postsynapse GO:0099186 IEA
Biological Process
    synapse assembly GO:0007416 IEA
    vesicle-mediated transport GO:0016192 IDA
    cerebellar Purkinje cell layer morphogenesis GO:0021692 IEA
    actin cytoskeleton organization GO:0030036 IBA
    adult behavior GO:0030534 IEA
    multicellular organism growth GO:0035264 IEA
    actin filament capping GO:0051693 IEA
    regulation of postsynaptic specialization assembly GO:0099150 IEA
    postsynapse organization GO:0099173 IEA
Subcellular Localization
    extracellular space GO:0005615 HDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    spectrin GO:0008091 IDA
    apical plasma membrane GO:0016324 IEA
    cell junction GO:0030054 IBA
    cortical actin cytoskeleton GO:0030864 IBA
    paranodal junction GO:0033010 IEA
    cell projection GO:0042995 IBA
    neuronal cell body GO:0043025 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    parallel fiber to Purkinje cell synapse GO:0098688 IEA
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 IEA
    postsynaptic spectrin-associated cytoskeleton GO:0099189 IEA
 Experiment description of studies that identified SPTBN2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPTBN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 PPP1CB 5500
Affinity Capture-MS Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 MARCKS 4082
Proximity Label-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 CCDC113  
Affinity Capture-MS Homo sapiens
7 RPA2 6118
Proximity Label-MS Homo sapiens
8 Calml3  
Affinity Capture-MS Mus musculus
9 APC  
Two-hybrid Homo sapiens
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 LAMP2 3920
Proximity Label-MS Homo sapiens
12 SOX2  
Affinity Capture-MS Homo sapiens
13 ILF2 3608
Co-fractionation Homo sapiens
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 Aurkb  
Affinity Capture-MS Mus musculus
16 B3GAT1  
Proximity Label-MS Homo sapiens
17 CORO1B 57175
Co-fractionation Homo sapiens
18 NOLC1 9221
Affinity Capture-MS Homo sapiens
19 IQGAP1 8826
Affinity Capture-MS Homo sapiens
20 PRC1 9055
Affinity Capture-MS Homo sapiens
21 CALD1 800
Affinity Capture-MS Homo sapiens
22 ACTR1A 10121
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
23 KIFAP3 22920
Affinity Capture-MS Homo sapiens
24 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MECP2 4204
Affinity Capture-MS Homo sapiens
26 RAB35 11021
Proximity Label-MS Homo sapiens
27 TFCP2 7024
Affinity Capture-MS Homo sapiens
28 ASF1A 25842
Affinity Capture-MS Homo sapiens
29 C18orf21  
Affinity Capture-MS Homo sapiens
30 DYNLT1 6993
Proximity Label-MS Homo sapiens
31 LAMTOR1 55004
Proximity Label-MS Homo sapiens
32 LMAN1 3998
Proximity Label-MS Homo sapiens
33 EXOC2 55770
Co-fractionation Homo sapiens
34 CHMP4C 92421
Affinity Capture-MS Homo sapiens
35 MYO18A 399687
Affinity Capture-MS Homo sapiens
36 RPS10 6204
Cross-Linking-MS (XL-MS) Homo sapiens
37 LYN 4067
Proximity Label-MS Homo sapiens
38 ATAD2 29028
Affinity Capture-MS Homo sapiens
39 DNAJC5 80331
Proximity Label-MS Homo sapiens
40 CDH1 999
Proximity Label-MS Homo sapiens
41 NCAPD3 23310
Co-fractionation Homo sapiens
42 YAP1 10413
Affinity Capture-MS Homo sapiens
43 MYH9 4627
Affinity Capture-MS Homo sapiens
44 SSUH2  
Affinity Capture-MS Homo sapiens
45 ADRB2  
Two-hybrid Homo sapiens
46 ACTB 60
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
47 CHMP4B 128866
Affinity Capture-MS Homo sapiens
48 CTDSPL 10217
Proximity Label-MS Homo sapiens
49 DIRAS3  
Proximity Label-MS Homo sapiens
50 ANLN 54443
Affinity Capture-MS Homo sapiens
51 Myh9 17886
Affinity Capture-MS Mus musculus
52 SORT1 6272
Affinity Capture-MS Homo sapiens
53 ECT2 1894
Affinity Capture-MS Homo sapiens
54 SIRT7  
Affinity Capture-MS Homo sapiens
55 LAMP1 3916
Proximity Label-MS Homo sapiens
56 ZC3HC1  
Affinity Capture-MS Homo sapiens
57 MYO19  
Affinity Capture-MS Homo sapiens
58 ARF6 382
Proximity Label-MS Homo sapiens
59 MYO1C 4641
Affinity Capture-MS Homo sapiens
60 SGTB  
Affinity Capture-MS Homo sapiens
61 SYNPO 11346
Affinity Capture-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 C6 729
Cross-Linking-MS (XL-MS) Homo sapiens
64 Bod1  
Affinity Capture-MS Mus musculus
65 MRPS36 92259
Affinity Capture-MS Homo sapiens
66 RHOB 388
Proximity Label-MS Homo sapiens
67 KRAS 3845
Proximity Label-MS Homo sapiens
68 FLOT1 10211
Proximity Label-MS Homo sapiens
69 CCDC8  
Affinity Capture-MS Homo sapiens
70 SPTBN1 6711
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
71 SPTAN1 6709
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
72 KIF14 9928
Affinity Capture-MS Homo sapiens
73 ABRA  
Affinity Capture-MS Homo sapiens
74 Myo1c 17913
Affinity Capture-MS Mus musculus
75 IKBKG 8517
Affinity Capture-MS Homo sapiens
76 LCK 3932
Proximity Label-MS Homo sapiens
77 CAPZA2 830
Affinity Capture-MS Homo sapiens
78 MLH1 4292
Co-fractionation Homo sapiens
79 ERGIC1 57222
Proximity Label-MS Homo sapiens
80 C11orf52 91894
Proximity Label-MS Homo sapiens
81 MBNL1 4154
Affinity Capture-MS Homo sapiens
82 RAB2A 5862
Proximity Label-MS Homo sapiens
83 FLNA 2316
Affinity Capture-MS Homo sapiens
84 PINK1  
Affinity Capture-MS Homo sapiens
85 ANK3  
Proximity Label-MS Homo sapiens
86 RAB5C 5878
Proximity Label-MS Homo sapiens
87 BHLHE23  
Affinity Capture-MS Homo sapiens
88 CCR1  
Affinity Capture-MS Homo sapiens
89 PRMT1 3276
Affinity Capture-MS Homo sapiens
90 CAV1 857
Proximity Label-MS Homo sapiens
91 UNC13B 10497
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
92 KDM5C  
Co-fractionation Homo sapiens
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