Gene description for NCAPD3
Gene name non-SMC condensin II complex, subunit D3
Gene symbol NCAPD3
Other names/aliases CAP-D3
CAPD3
hCAP-D3
hHCP-6
hcp-6
Species Homo sapiens
 Database cross references - NCAPD3
ExoCarta ExoCarta_23310
Entrez Gene 23310
HGNC 28952
MIM 609276
UniProt P42695  
 NCAPD3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NCAPD3
Molecular Function
    methylated histone binding GO:0035064 IDA
    protein binding GO:0005515 IPI
Biological Process
    mitotic cell cycle GO:0000278 TAS
    mitotic chromosome condensation GO:0007076 IDA
    cell division GO:0051301 IEA
Subcellular Localization
    membrane GO:0016020 IDA
    nucleoplasm GO:0005654 TAS
    nuclear condensin complex GO:0000799 IDA
    germinal vesicle GO:0042585 IEA
    nuclear pericentric heterochromatin GO:0031618 IDA
 Experiment description of studies that identified NCAPD3 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NCAPD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRSS23 11098
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NCAPD3 is involved
PathwayEvidenceSource
Condensation of Prophase Chromosomes TAS Reactome





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