Gene description for NCAPD3
Gene name non-SMC condensin II complex, subunit D3
Gene symbol NCAPD3
Other names/aliases CAP-D3
CAPD3
hCAP-D3
hHCP-6
hcp-6
Species Homo sapiens
 Database cross references - NCAPD3
ExoCarta ExoCarta_23310
Vesiclepedia VP_23310
Entrez Gene 23310
HGNC 28952
MIM 609276
UniProt P42695  
 NCAPD3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NCAPD3
Molecular Function
    protein binding GO:0005515 IPI
    methylated histone binding GO:0035064 IDA
    histone binding GO:0042393 IBA
Biological Process
    mitotic chromosome condensation GO:0007076 IBA
    mitotic chromosome condensation GO:0007076 IDA
    mitotic chromosome condensation GO:0007076 IMP
    meiotic chromosome condensation GO:0010032 IBA
    cell division GO:0051301 IEA
    positive regulation of chromosome segregation GO:0051984 ISO
    positive regulation of chromosome separation GO:1905820 ISO
    positive regulation of chromosome condensation GO:1905821 ISS
Subcellular Localization
    condensed chromosome, centromeric region GO:0000779 IBA
    condensed nuclear chromosome GO:0000794 ISO
    condensin complex GO:0000796 IBA
    condensin complex GO:0000796 IDA
    condensin complex GO:0000796 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    pericentric heterochromatin GO:0005721 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified NCAPD3 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NCAPD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 MCM3 4172
Co-fractionation Homo sapiens
4 SENP3 26168
Affinity Capture-MS Homo sapiens
5 LGR4 55366
Affinity Capture-MS Homo sapiens
6 PAXIP1  
Affinity Capture-MS Homo sapiens
7 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CALM1 801
Affinity Capture-MS Homo sapiens
10 PSMD4 5710
Co-fractionation Homo sapiens
11 CLTB 1212
Co-fractionation Homo sapiens
12 FAXC  
Affinity Capture-MS Homo sapiens
13 PNKD  
Affinity Capture-MS Homo sapiens
14 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
15 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
16 USP39 10713
Co-fractionation Homo sapiens
17 POU5F1  
Affinity Capture-MS Homo sapiens
18 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SMC2 10592
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
20 SEMA4C 54910
Affinity Capture-MS Homo sapiens
21 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DGCR2 9993
Affinity Capture-MS Homo sapiens
23 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MYCN  
Affinity Capture-MS Homo sapiens
25 RIC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SMC4 10051
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 YWHAB 7529
Co-fractionation Homo sapiens
28 PIP4K2C 79837
Co-fractionation Homo sapiens
29 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
30 C19orf38  
Affinity Capture-MS Homo sapiens
31 MRPL45 84311
Affinity Capture-MS Homo sapiens
32 CTDP1 9150
Affinity Capture-MS Homo sapiens
33 Ncapg2  
Affinity Capture-MS Mus musculus
34 PARK2  
Affinity Capture-MS Homo sapiens
35 LRRC25  
Affinity Capture-MS Homo sapiens
36 COMTD1 118881
Affinity Capture-MS Homo sapiens
37 NTRK1 4914
Affinity Capture-MS Homo sapiens
38 RPL31 6160
Proximity Label-MS Homo sapiens
39 GPR182  
Affinity Capture-MS Homo sapiens
40 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 BSND  
Affinity Capture-MS Homo sapiens
42 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 ENO1 2023
Affinity Capture-RNA Homo sapiens
44 HECTD1 25831
Affinity Capture-MS Homo sapiens
45 SDF4 51150
Affinity Capture-MS Homo sapiens
46 ACTBL2 345651
Affinity Capture-MS Homo sapiens
47 CEBPA  
Protein-peptide Homo sapiens
48 BIN1 274
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CACNG4  
Affinity Capture-MS Homo sapiens
50 KDF1  
Affinity Capture-MS Homo sapiens
51 CISD3 284106
Affinity Capture-MS Homo sapiens
52 TEKT1  
Two-hybrid Homo sapiens
53 EFNA4  
Affinity Capture-MS Homo sapiens
54 CXCR4 7852
Affinity Capture-MS Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
56 BTNL9  
Affinity Capture-MS Homo sapiens
57 YWHAZ 7534
Co-fractionation Homo sapiens
58 NCAPH2  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
59 CD6 923
Affinity Capture-MS Homo sapiens
60 FOXG1  
Affinity Capture-MS Homo sapiens
61 NR3C1 2908
Proximity Label-MS Homo sapiens
62 FOXL1  
Affinity Capture-MS Homo sapiens
63 EIF2S2 8894
Co-fractionation Homo sapiens
64 RIPK4  
Affinity Capture-MS Homo sapiens
65 SPTBN1 6711
Co-fractionation Homo sapiens
66 NXF1 10482
Affinity Capture-RNA Homo sapiens
67 PDCD4 27250
Co-fractionation Homo sapiens
68 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 C3orf18  
Affinity Capture-MS Homo sapiens
70 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
71 GPR45  
Affinity Capture-MS Homo sapiens
72 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
73 Ssna1  
Affinity Capture-MS Mus musculus
74 DDRGK1 65992
Affinity Capture-MS Homo sapiens
75 NCAPG2 54892
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
76 XRCC3  
Affinity Capture-MS Homo sapiens
77 EPAS1  
Affinity Capture-MS Homo sapiens
78 NANOG  
Affinity Capture-MS Homo sapiens
79 Smc4 70099
Affinity Capture-MS Mus musculus
80 ZRANB1 54764
Affinity Capture-MS Homo sapiens
81 NES 10763
Affinity Capture-MS Homo sapiens
82 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
83 SPTAN1 6709
Co-fractionation Homo sapiens
84 SCYL3 57147
Affinity Capture-MS Homo sapiens
85 TP53 7157
Affinity Capture-MS Homo sapiens
86 OPALIN  
Affinity Capture-MS Homo sapiens
87 SPTBN2 6712
Co-fractionation Homo sapiens
88 MEN1 4221
Affinity Capture-MS Homo sapiens
89 MAGEA9  
Affinity Capture-MS Homo sapiens
90 ESR1  
Affinity Capture-Western Homo sapiens
91 SKAP1 8631
Affinity Capture-MS Homo sapiens
92 NLN 57486
Affinity Capture-MS Homo sapiens
93 RPS24 6229
Proximity Label-MS Homo sapiens
94 MOV10 4343
Affinity Capture-RNA Homo sapiens
95 ZUFSP 221302
Affinity Capture-MS Homo sapiens
96 KRAS 3845
Negative Genetic Homo sapiens
97 C9orf72  
Affinity Capture-MS Homo sapiens
98 PPP2R2A 5520
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
99 SPINT2 10653
Affinity Capture-MS Homo sapiens
100 PRSS23 11098
Two-hybrid Homo sapiens
101 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 HLA-C 3107
Affinity Capture-MS Homo sapiens
103 ESCO2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NCAPD3 is involved
PathwayEvidenceSource
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Condensation of Prophase Chromosomes TAS Reactome
M Phase TAS Reactome
Mitotic Prophase TAS Reactome





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