Gene description for BIN1
Gene name bridging integrator 1
Gene symbol BIN1
Other names/aliases AMPH2
AMPHL
SH3P9
Species Homo sapiens
 Database cross references - BIN1
ExoCarta ExoCarta_274
Entrez Gene 274
HGNC 1052
MIM 601248
UniProt O00499  
 BIN1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for BIN1
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    tau protein binding GO:0048156 IPI
    RNA polymerase binding GO:0070063 IPI
    protein complex binding GO:0032403 IEA
    GTPase binding GO:0051020 IEA
Biological Process
    regulation of cell cycle arrest GO:0071156 IDA
    positive regulation of endocytosis GO:0045807 IEA
    positive regulation of astrocyte differentiation GO:0048711 IMP
    viral process GO:0016032 IEA
    positive regulation of GTPase activity GO:0043547 IEA
    lipid tube assembly GO:0060988 IMP
    cell proliferation GO:0008283 TAS
    regulation of neuron differentiation GO:0045664 IMP
    muscle cell differentiation GO:0042692 IEA
    endocytosis GO:0006897 IEA
    positive regulation of apoptotic process GO:0043065 IMP
Subcellular Localization
    actin cytoskeleton GO:0015629 TAS
    axon GO:0030424 IDA
    nucleus GO:0005634 IEA
    node of Ranvier GO:0033268 ISS
    axon initial segment GO:0043194 ISS
    synaptic vesicle GO:0008021 IEA
    membrane GO:0016020 IDA
    I band GO:0031674 ISS
    cytoplasm GO:0005737 IDA
    T-tubule GO:0030315 ISS
    varicosity GO:0043196 IEA
    Z disc GO:0030018 ISS
    lipid tube GO:0060987 IMP
    axon terminus GO:0043679 IEA
    cerebellar mossy fiber GO:0044300 IEA
 Experiment description of studies that identified BIN1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BIN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SH3GL2  
Reconstituted Complex Homo sapiens
2 ITGA1 3672
Two-hybrid Homo sapiens
3 ITGA3 3675
Two-hybrid Homo sapiens
4 CUTL1  
Two-hybrid Homo sapiens
5 SYN1  
Invivo Homo sapiens
6 SH3GLB1 51100
Reconstituted Complex Homo sapiens
7 RIN3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 BIN1 274
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 SYNJ1  
Invitro Homo sapiens
10 AMPH  
Affinity Capture-Western Homo sapiens
11 SNX4 8723
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 PLD2 5338
Phenotypic Suppression Homo sapiens
Reconstituted Complex Homo sapiens
13 PTK2 5747
Invitro Homo sapiens
14 AP2A1 160
Invitro Homo sapiens
15 DNM1 1759
Invitro Homo sapiens
Invitro Homo sapiens
16 PLD1 5337
Phenotypic Suppression Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which BIN1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here