Gene description for PLD2
Gene name phospholipase D2
Gene symbol PLD2
Other names/aliases -
Species Homo sapiens
 Database cross references - PLD2
ExoCarta ExoCarta_5338
Vesiclepedia VP_5338
Entrez Gene 5338
HGNC 9068
MIM 602384
UniProt O14939  
 PLD2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PLD2
Molecular Function
    phospholipase D activity GO:0004630 IBA
    phospholipase D activity GO:0004630 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 TAS
    cytoskeleton organization GO:0007010 TAS
    small GTPase-mediated signal transduction GO:0007264 TAS
    phospholipid catabolic process GO:0009395 IBA
    synaptic vesicle recycling GO:0036465 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    regulation of vesicle-mediated transport GO:0060627 IBA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    cytoplasmic vesicle GO:0031410 IBA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified PLD2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PLD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAMP3  
Affinity Capture-MS Homo sapiens
2 CACNG5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PRKCD 5580
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 HACD3 51495
Affinity Capture-MS Homo sapiens
5 CDH8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 Pparg  
Affinity Capture-MS Mus musculus
7 CRYL1 51084
Affinity Capture-MS Homo sapiens
8 SRPR 6734
Affinity Capture-MS Homo sapiens
9 EGLN1 54583
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 VHL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 URI1  
Affinity Capture-MS Homo sapiens
12 BAG5 9529
Affinity Capture-MS Homo sapiens
13 TCTEX1D2  
Affinity Capture-MS Homo sapiens
14 TEX264 51368
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ANKMY2 57037
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
16 HSPA2 3306
Affinity Capture-MS Homo sapiens
17 ISYNA1 51477
Affinity Capture-MS Homo sapiens
18 OPRM1 4988
Affinity Capture-Western Homo sapiens
19 SRC 6714
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 HERC4 26091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 POU5F1  
Affinity Capture-MS Homo sapiens
22 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PLCG1 5335
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 PEA15 8682
Affinity Capture-Western Homo sapiens
25 ATG7 10533
Affinity Capture-MS Homo sapiens
26 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CIB2  
Affinity Capture-MS Homo sapiens
28 ASRGL1 80150
Affinity Capture-MS Homo sapiens
29 DYNLT1 6993
Affinity Capture-MS Homo sapiens
30 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HSPA8 3312
Affinity Capture-MS Homo sapiens
32 C15orf59  
Affinity Capture-MS Homo sapiens
33 BSG 682
Affinity Capture-MS Homo sapiens
34 UGGT1 56886
Affinity Capture-MS Homo sapiens
35 CAMK2A 815
Affinity Capture-MS Homo sapiens
36 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
37 Actb 81822
Reconstituted Complex Rattus norvegicus
38 DNAJB1 3337
Affinity Capture-MS Homo sapiens
39 PPP5C 5536
Affinity Capture-MS Homo sapiens
40 CACNG7  
Affinity Capture-MS Homo sapiens
41 TSPAN3 10099
Affinity Capture-MS Homo sapiens
42 TUBB2B 347733
Affinity Capture-MS Homo sapiens
43 RPS6KB1 6198
Affinity Capture-Western Homo sapiens
44 TUBA1C 84790
Affinity Capture-MS Homo sapiens
45 PCCA 5095
Affinity Capture-MS Homo sapiens
46 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 THBS3 7059
Affinity Capture-MS Homo sapiens
49 GAPDH 2597
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 TTC9C 283237
Affinity Capture-MS Homo sapiens
51 VCPIP1 80124
Affinity Capture-MS Homo sapiens
52 SDF4 51150
Affinity Capture-MS Homo sapiens
53 DNAJB5  
Affinity Capture-MS Homo sapiens
54 BIN1 274
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
55 PIPSL 266971
Affinity Capture-MS Homo sapiens
56 EIF4A2 1974
Affinity Capture-MS Homo sapiens
57 TUBA3C 7278
Affinity Capture-MS Homo sapiens
58 BAG3 9531
Affinity Capture-MS Homo sapiens
59 ANLN 54443
Affinity Capture-MS Homo sapiens
60 DNAJB14  
Affinity Capture-MS Homo sapiens
61 TUBB 203068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TOMM34 10953
Affinity Capture-MS Homo sapiens
63 SPAG1 6674
Affinity Capture-MS Homo sapiens
64 CXCR4 7852
Affinity Capture-MS Homo sapiens
65 XPO1 7514
Affinity Capture-MS Homo sapiens
66 BAG2 9532
Affinity Capture-MS Homo sapiens
67 CAV2 858
Co-fractionation Homo sapiens
68 ASIC2  
Affinity Capture-MS Homo sapiens
69 CCDC107  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SPC24 147841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 CUL3 8452
Affinity Capture-MS Homo sapiens
72 TUBB4B 10383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 EGFR 1956
Affinity Capture-Western Homo sapiens
74 ARF1 375
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
75 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
76 AQP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
77 TMEM74  
Affinity Capture-MS Homo sapiens
78 TMEM169  
Affinity Capture-MS Homo sapiens
79 VBP1 7411
Affinity Capture-MS Homo sapiens
80 AMPH 273
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
81 TUBA4A 7277
Affinity Capture-MS Homo sapiens
82 FKBP5 2289
Affinity Capture-MS Homo sapiens
83 KIF1BP 26128
Affinity Capture-MS Homo sapiens
84 MCMBP 79892
Affinity Capture-MS Homo sapiens
85 MTOR 2475
Affinity Capture-Western Homo sapiens
86 HSP90AA2P 3324
Affinity Capture-MS Homo sapiens
87 RPAP3 79657
Affinity Capture-MS Homo sapiens
88 PDP1 54704
Affinity Capture-MS Homo sapiens
89 BAG4  
Affinity Capture-MS Homo sapiens
90 HIF1A 3091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
91 STUB1 10273
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 FTL 2512
Affinity Capture-MS Homo sapiens
93 HNRNPH2 3188
Affinity Capture-MS Homo sapiens
94 TTC1 7265
Affinity Capture-MS Homo sapiens
95 TOMM70A 9868
Affinity Capture-MS Homo sapiens
96 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 RPTOR 57521
Affinity Capture-Western Homo sapiens
99 VSIG8  
Affinity Capture-MS Homo sapiens
100 MAGEA9  
Affinity Capture-MS Homo sapiens
101 PTGER3  
Affinity Capture-MS Homo sapiens
102 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 SKAP1 8631
Affinity Capture-MS Homo sapiens
104 PPP4R1L  
Affinity Capture-MS Homo sapiens
105 PLD3 23646
Affinity Capture-Western Homo sapiens
106 BSND  
Affinity Capture-MS Homo sapiens
107 DNAJB4 11080
Affinity Capture-MS Homo sapiens
108 PRKCA 5578
Affinity Capture-Western Homo sapiens
109 ZCCHC10  
Affinity Capture-MS Homo sapiens
110 SSSCA1 10534
Affinity Capture-MS Homo sapiens
111 CDK5RAP3 80279
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 PCCB 5096
Affinity Capture-MS Homo sapiens
113 PIH1D1  
Affinity Capture-MS Homo sapiens
114 PTGES3 10728
Affinity Capture-MS Homo sapiens
115 DDI2 84301
Affinity Capture-MS Homo sapiens
116 DNAJA1 3301
Affinity Capture-MS Homo sapiens
117 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
118 LURAP1  
Affinity Capture-MS Homo sapiens
119 HSPA1B 3304
Affinity Capture-MS Homo sapiens
120 ALDOA 226
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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